抗Human BMP-6抗体(Anti-Human BMP-6 antibody)
掲載日情報：2021/01/28 現在Webページ番号：28414
Human BMP-6に対する抗体(Anti-Human BMP-6 )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月19日 00時00分現在]
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Anti-Human BMP-6 MAb (Clone 74219) |
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[在庫・価格 ：2024年05月19日 00時00分現在]
Anti-Human BMP-6 MAb (Clone 74219)
文献数： 0
- 商品コード：MAB507-100
- メーカー：RSD
- 包装：100μg
- 価格：¥101,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
別名：BMP6 クローン：74219 Genbank No： 654 Protein Accession No： Q4VBA3 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human BMP-6 MAb (Clone 74219)
文献数： 0
- 商品コード：MAB507-500
- メーカー：RSD
- 包装：500μg
- 価格：¥232,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Human BMP-6 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF507，スタンダードとして#507-BP-020を用いる。 別名：BMP6 クローン：74219 Genbank No： 654 Protein Accession No： Q4VBA3 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human BMP-6 MAb (Clone 74219)
文献数： 0
- 商品コード：MAB507-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 別名：BMP6 クローン：74219 Genbank No： 654 Protein Accession No： Q4VBA3 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | |||||||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human BMP‑6Arg296-His428Accession # Q4VBA3 |
| Source | Monoclonal Mouse IgG2B Clone # 74219 |
| Purification | Protein A or G purified from ascites |
| Specificity | Detects human BMP-6 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BMP‑2, rhBMP‑4, rhBMP‑5, or rhBMP‑7 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Human BMP‑6 (Catalog # 507-BP) under non-reducing conditions only |
| Human BMP-6 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human BMP‑6 Antibody (Catalog #MAB507 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human BMP‑6 Biotinylated Antibody (Catalog #BAF507 ) |
| ELISA Standard | Recombinant Human BMP-6 Protein (Catalog #507-BP ) | |
| Neutralization | Measured by its ability to neutralize BMP‑6-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 300 ng/mL Recombinant Human BMP‑6. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
 click image to view larger | Alkaline Phosphatase Production Induced by BMP‑6 and Neutralization by Human BMP‑6 Antibody. Recombinant Human BMP‑6 (Catalog # 507-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑6 (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP‑6 Monoclonal Antibody (Catalog # MAB507). The ND50 is typically 0.5-2.0 µg/mL. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: BMP-6 | Human BMP-6 is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human and mouse BMP-6 shares 96% amino acid sequence identity. BMP-6 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. Biologically active BMP-6 is a disulfide-linked homodimer of the carboxy-terminal 132 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-6 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. Based on amino acid sequence similarity, BMP-5, -6, -7, and -8 are in the same subgroup. |
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References
| Ebendal, T. et al. (1998) J. Neurosci. Res. 51:139. | |
| Reddi, A.H. (1998) Nature Biotechnology 16:247. | |
| Long Name: | Bone Morphogenetic Protein 6 |
| Entrez Gene IDs: | 654 (Human); 12161 (Mouse); 25644 (Rat) |
| Alternate Names: | BMP6; BMP-6; bone morphogenetic protein 6; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine; VG-1-R; VG-1-related protein; Vg1-related sequence; VGR; VGR1; Vgr-1 |
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Related Research Areas
| BMP Family | ||
| BMPs (Bone Morphogenetic Proteins) | ||
| Early Mesodermal Lineage Markers | ||
| Molecules Secreted by VSMC | ||
| Negative Regulators of EMT | ||
| TGF-beta Superfamily | ||
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| Neutralization | |
|---|---|
 | Alkaline Phosphatase Production Induced by BMP‑6 and Neutralization by Human BMP‑6 Antibody. Recombinant Human BMP‑6 (Catalog # 507-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑6 (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP‑6 Monoclonal Antibody (Catalog # MAB507). The ND50 is typically 0.5-2.0 µg/mL. |
追加しました。
Related Product & Information
| Long Name | Bone Morphogenetic Protein 6 |
|---|---|
| Entrez Gene IDs | 654 (Human); 12161 (Mouse); 25644 (Rat) |
| Background | BMP-6 |
| background_content | Background: BMP-6 Human BMP-6 is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human and mouse BMP-6 shares 96% amino acid sequence identity. BMP-6 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. Biologically active BMP-6 is a disulfide-linked homodimer of the carboxy-terminal 132 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-6 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. Based on amino acid sequence similarity, BMP-5, -6, -7, and -8 are in the same subgroup. |
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Citations
- TNF? drives pulmonary arterial hypertension by suppressing the BMP type-II receptor and altering NOTCH signalling
Authors: LA Hurst, BJ Dunmore, L Long, A Crosby, R Al-Lamki, J Deighton, M Southwood, X Yang, MZ Nikolic, B Herrera, GJ Inman, JR Bradley, AA Rana, PD Upton, NW Morrell
Nat Commun, 2017;8(0):14079.
Species: Mouse
Sample Type: Whole Cells
Application: Neut - Regulation of TMPRSS6 by BMP6 and iron in human cells and mice.
Authors: Meynard D, Vaja V, Sun CC, Corradini E, Chen S, Lopez-Otin C, Grgurevic L, Hong CC, Stirnberg M, Gutschow M, Vukicevic S, Babitt JL, Lin HY
Blood, 2011;118(3):747-56.
Species: Mouse
Sample Type: In Vivo
Application: In vivo - Bone morphogenetic protein-6 promotes osteoblastic prostate cancer bone metastases through a dual mechanism.
Authors: Dai J, Keller J, Zhang J, Lu Y, Yao Z, Keller ET
Cancer Res., 2005;65(18):8274-85.
Species: Human
Sample Type: Cell Lysates
Application: WB
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