抗Human IL-15抗体(Anti-Human IL-15 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL-15Asn49-Ser162Accession # P40933
Source	Monoclonal Mouse IgG1 Clone # 34559
Purification	Protein A or G purified from ascites
Specificity	Detects human IL-15 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2, recombinant mouse IL-15, or rhIL-21 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL‑15 (Catalog # 247-IL)
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	Human peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Neutralization	Measured by its ability to neutralize IL‑15-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 5 ng/mL Recombinant Human IL‑15.

Neutralization

Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. Recombinant Human IL‑15 (Catalog # 247-ILB) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL‑15 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑15 Monoclonal Antibody (Catalog # MAB2471). The ND50 is typically 0.1-0.5 µg/mL.

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Related Product & Information

Long Name	Interleukin 15
Entrez Gene IDs	3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
Background	IL-15
background_content	Background: IL-15 Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1‑3). Mature human IL‑15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11‑13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

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Citations

1. Human central nervous system astrocytes support survival and activation of B cells: implications for MS pathogenesis
   Authors: H Touil, A Kobert, N Lebeurrier, A Rieger, P Saikali, C Lambert, L Fawaz, CS Moore, A Prat, J Gommerman, JP Antel, Y Itoyama, I Nakashima, A Bar-Or,
   J Neuroinflammation, 2018;15(1):114.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. MiR-205/YAP1 in Activated Fibroblasts of Breast Tumor Promotes VEGF-independent Angiogenesis through STAT3 Signaling
   Authors: YE Du, G Tu, G Yang, G Li, D Yang, L Lang, L Xi, K Sun, Y Chen, K Shu, H Liao, M Liu, Y Hou
   Theranostics, 2017;7(16):3972-3988.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Characterization of Interleukin-15-Transpresenting Dendritic Cells for Clinical Use
   Authors: JMJ Van den Be, ELJM Smits, M Versteven, H De Reu, ZN Berneman, VFI Van Tendel, E Lion
   J Immunol Res, 2017;2017(0):1975902.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. A Refractory Celiac Patient Successfully Treated With Mesenchymal Stem Cell Infusions
   Authors: R Ciccociopp, A Gallia, MA Avanzini, E Betti, C Picone, A Vanoli, C Paganini, F Biagi, R Maccario, GR Corazza
   Mayo Clin Proc, 2016;0(0):.
   Species: Human
   Sample Type: BALF
   Application: WB


5. Interleukin-15 Dendritic Cells Harness NK Cell Cytotoxic Effector Function in a Contact- and IL-15-Dependent Manner.
   Authors: Anguille S, Van Acker H, Van den Bergh J, Willemen Y, Goossens H, Van Tendeloo V, Smits E, Berneman Z, Lion E
   PLoS ONE, 2015;10(5):e0123340.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Type I IFNs and IL-18 regulate the antiviral response of primary human gammadelta T cells against dendritic cells infected with Dengue virus.
   Authors: Tsai C, Liong K, Gunalan M, Li N, Lim D, Fisher D, Macary P, Leo Y, Wong S, Puan K, Wong S
   J Immunol, 2015;194(8):3890-900.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. IL15RA drives antagonistic mechanisms of cancer development and immune control in lymphocyte-enriched triple-negative breast cancers.
   Authors: Marra P, Mathew S, Grigoriadis A, Wu Y, Kyle-Cezar F, Watkins J, Rashid M, De Rinaldis E, Hessey S, Gazinska P, Hayday A, Tutt A
   Cancer Res, 2014;74(17):4908-21.
   Species: Human
   Sample Type: Whole Cells
   Application: Blocking


8. Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection.
   Authors: Muntasell A, Magri G, Pende D, Angulo A, Lopez-Botet M
   Blood, 2010;115(25):5170-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. Juxtacrine function of interleukin-15/interleukin-15 receptor system in tumour derived human B-cell lines.
   Authors: Tsukamoto K, Huang YC, Dorsey WC, Carns B, Sharma V
   Clin. Exp. Immunol., 2006;146(3):559-66.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Decreased IL-15 may contribute to elevated IgE and acute inflammation in atopic dermatitis.
    Authors: Ong PY, Hamid QA, Travers JB, Strickland I, Al Kerithy M, Boguniewicz M, Leung DY
    J. Immunol., 2002;168(1):505-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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