抗Mouse CXCL2/MIP-2抗体(Anti-Mouse CXCL2/MIP-2 antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CXCL2/GRO beta /MIP-2/CINC-3Ala28-Asn100Accession # P10889
Source	Monoclonal Rat IgG2B Clone # 40605
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse CXCL2 in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) CXCL2/GRO beta, rhCXCL3/GRO gamma, or recombinant mouse CXCL1/KC.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse CXCL2/GRO beta /MIP-2/CINC-3 (Catalog # 452-M2) under non-reducing conditions only
Mouse CXCL2/MIP-2 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Antibody (Catalog #MAB452 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Biotinylated Antibody (Catalog #BAF452 )
ELISA Standard		Recombinant Mouse CXCL2/MIP-2 Protein (Catalog #452-M2 )
Neutralization	Measured by its ability to neutralize CXCL2/GRO beta /MIP‑2/CINC‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/GRO beta /MIP‑2/CINC‑3.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Chemotaxis Induced by CXCL2/MIP‑2 and Neutralization by Mouse CXCL2/MIP‑2 Antibody.Recombinant Mouse CXCL2/MIP‑2 (Catalog # 452-M2) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL2/MIP‑2 Monoclonal Antibody (Catalog # MAB452). The ND50 is typically 1-4 µg/mL.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL2/GRO beta/MIP-2/CINC-3	Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines. MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs. Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.
Entrez Gene IDs:	2920 (Human); 20310 (Mouse); 114105 (Rat)
Alternate Names:	chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2

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Related Research Areas

Chemokines and Receptors in Angiogenesis

CXC Chemokines and Receptors

Inflammatory Mediators

Macrophage Activation Markers

Molecules Secreted by VSMC

Neuroinflammation

Mucin 1 protects against severe Streptococcus pneumoniae infection Authors: P Dhar, GZ Ng, EM Dunne, P Sutton Virulence, 2017;0(0):0. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Capture

Neutralization

Chemotaxis Induced by CXCL2/MIP‑2 and Neutralization by Mouse CXCL2/MIP‑2 Antibody. Recombinant Mouse CXCL2/MIP‑2 (Catalog #452-M2) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL2/MIP‑2 Monoclonal Antibody (Catalog # MAB452). The ND50 is typically 1-4 µg/mL.

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Related Product & Information

Entrez Gene IDs	2920 (Human); 20310 (Mouse); 114105 (Rat)
Background	CXCL2/GRO beta/MIP-2/CINC-3
background_content	Background: CXCL2/GRO beta/MIP-2/CINC-3 Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines. MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs. Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.

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Citations

1. Mucin 1 protects against severe Streptococcus pneumoniae infection
   Authors: P Dhar, GZ Ng, EM Dunne, P Sutton
   Virulence, 2017;0(0):0.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Capture


2. The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection
   Authors: Z Pang, RD Junkins, AJ MacNeil, C McCormick, Z Cheng, WM Chen, TJ Lin
   J. Leukoc. Biol., 2017;0(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA - Capture


3. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
   Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
   Cell. Physiol. Biochem., 2017;42(5):1961-1972.
   Species: Mouse
   Sample Type: Serum
   


4. Absence of DCIR1 reduces the mortality rate of endotoxemic hepatitis in mice
   Authors: T Ishiguro, T Fukawa, K Akaki, K Nagaoka, T Takeda, Y Iwakura, K Inaba, K Takahara
   Eur. J. Immunol, 2017;0(0):.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA Capture


5. Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice
   Authors: Clara Jana-Lui Busch
   Hepatology, 2016;0(0):.
   Species: Mouse
   Sample Type: Peritoneal Lavage Fluid
   Application: ELISA Capture


6. Patrolling monocytes promote intravascular neutrophil activation and glomerular injury in the acutely inflamed glomerulus
   Proc Natl Acad Sci USA, 2016;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


7. Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
   Authors: Kowalewska P, Patrick A, Fox-Robichaud A
   PLoS ONE, 2014;9(9):e104537.
   Species: Mouse
   Sample Type: In Vivo
   Application: IHC Fresh


8. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
   Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
   J Immunol, 2013;190(10):5178-86.
   Species: Mouse
   Sample Type: BALF
   Application: ELISA Capture


9. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
   Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
   J Immunol, 2013;190(10):5329-36.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Capture


10. CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis.
    Authors: Roy M, Richard JF, Dumas A, Vallieres L
    J Neuroinflammation, 2012;9(0):18.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo

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