抗TNF Receptor Ⅰ抗体(Anti-TNF Receptor Ⅰ, Mouse, Rat-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse TNF RIIle22-Ala212Accession # P25118
Source	Monoclonal Rat IgG1 Clone # 47803
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse TNF RI in ELISAs and Western blots. In ELISAs, no cross-reactivity was observed with recombinant human (rh) TNF RI, rhTNF RII, recombinant mouse TNF RII, rhTNF-alpha, recombinant rat TNF-alpha, recombinant porcine TNF-alpha, or rhTNF-beta.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Mouse TNF RI/TNFRSF1A Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse/Rat TNF RI/TNFRSF1A Antibody (Catalog #MAB425 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse TNF RI/TNFRSF1A Biotinylated Antibody (Catalog #BAF425 )
ELISA Standard		Recombinant Mouse sTNF RI/TNFRSF1A Protein (Catalog #425-R1 )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

目次に戻る

Data Examples

Western Blot	click image to view larger	Detection of Mouse and Rat TNF RI/TNFRSF1A by Western Blot. Western blot shows lysates of C6 rat glioma cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB425) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RI/TNFRSF1A	TNF RI functions as a receptor for TNF-alpha and TNF-beta. It is a member of the TNF receptor superfamily and is designated TNFRSF1A.
Long Name:	Tumor Necrosis Factor Receptor I
Entrez Gene IDs:	7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names:	CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

目次に戻る

Related Research Areas

Adipocytokines

Cell Structure and Signaling Molecules

Cytokine and Growth Factor Receptors on VSMC

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Angiogenesis

Diabetes

Diabetic Peripheral Neuropathy

EMT Induction

Endothelial Cell Markers

Neuroinflammation

Obesity

Platelet Cytokine and Growth Factor Receptors

Regulation of Apoptosis by TNF Superfamily Members

T Cell Cytokine Signaling

TNF Superfamily Receptors

Pharmacokinetic and Pharmacodynamic Characterisation of an Anti-Mouse TNF Receptor 1 Domain Antibody Formatted for In Vivo Half-Life Extension. Authors: Goodall L, Ovecka M, Rycroft D, Friel S, Sanderson A, Mistry P, Davies M, Stoop A PLoS ONE, 2015;10(9):e0137065. Species: Mouse Sample Type: Serum Application: ELISA Capture

Western Blot

Detection of Mouse and Rat TNF RI/TNFRSF1A by Western Blot. Western blot shows lysates of C6 rat glioma cell line and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB425) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

目次に戻る

Related Product & Information

Long Name	Tumor Necrosis Factor Receptor I
Entrez Gene IDs	7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Background	TNF RI/TNFRSF1A
background_content	Background: TNF RI/TNFRSF1A TNF RI functions as a receptor for TNF-alpha and TNF-beta. It is a member of the TNF receptor superfamily and is designated TNFRSF1A.

目次に戻る

Citations

1. Pharmacokinetic and Pharmacodynamic Characterisation of an Anti-Mouse TNF Receptor 1 Domain Antibody Formatted for In Vivo Half-Life Extension.
   Authors: Goodall L, Ovecka M, Rycroft D, Friel S, Sanderson A, Mistry P, Davies M, Stoop A
   PLoS ONE, 2015;10(9):e0137065.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA Capture


2. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


3. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
   Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
   J. Immunol., 2006;177(3):1967-74.
   Species: Mouse
   Sample Type: Plasma
   Application: ELISA Development


4. Sequential ELISA to profile multiple cytokines from small volumes.
   Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
   J. Immunol. Methods, 2005;302(1):172-81.
   Species: Mouse
   Sample Type: Plasma
   Application: ELISA Development


5. Selective macrophage suppression during sepsis.
   Authors: Ellaban E, Bolgos G, Remick D
   Cell. Immunol., 2004;231(1):103-11.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development

目次に戻る