抗Human CCL18/PARC抗体(Anti-Human CCL18/PARC antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CCL18/PARCAla21-Ala89Accession # P55774.1
Source	Monoclonal Mouse IgG1 Clone # 64507
Purification	Protein A or G purified from ascites
Specificity	Detects human CCL18/PARC in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human CCL3, 4, 14, 15, 23, recombinant mouse (rm) CCL3, rmCCL4, recombinant viral (rv) MIP-I or rvMIP‑II.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human CCL18/PARC (Catalog # 394-PA)
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	Human monocyte-derived dendritic cells treated with Recombinant Human IL‑4 (Catalog # 204-IL), Recombinant Human GM-CSF (Catalog # 215-GM), Recombinant Human TNF‑ alpha (Catalog # 210-TA), Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB), and LPS, fixed with paraformaldehyde and permeabilized with saponin
Human CCL18/PARC Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human CCL18/PARC Antibody (Catalog #MAB394 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human CCL18/PARC Biotinylated Antibody (Catalog #BAF394 )
ELISA Standard		Recombinant Human CCL18/PARC Protein (Catalog #394-PA )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL18/PARC	CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein 4 (MIP-4), and dendritic cell chemokine (DC‑CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10, and IL-13, and inhibited by IFN-gamma. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells but not monocytes or neutrophils.

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References

Adema, G. et al. (1997) Nature 387:713.
Kodelja, V. et al. (1998) J. Immunol. 160:141.
Entrez Gene IDs:	6362 (Human)
Alternate Names:	Alternative macrophage activation-associated CC chemokine 1; AMAC-1; AMAC1CC chemokine ligand 18; AMAC-1Small-inducible cytokine A18; CCL18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); CKb7; DC-CK1; DC-CK1CC chemokine PARC; DCCK1Macrophage inflammatory protein 4; Dendritic cell chemokine 1; MIP4; MIP-4; MIP-4C-C motif chemokine 18; PARC; PARCPulmonary and activation-regulated chemokine; SCYA18chemokine (C-C), dendritic; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated

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Related Research Areas

CC Chemokines and Receptors

Chemokines and Receptors

Macrophage Activation Markers

Multiplex serum protein analysis reveals potential mechanisms and markers of response to hyperimmune caprine serum in systemic sclerosis Authors: N Quillinan, KE Clark, B Youl, J Vernes, D McIntosh, S Haq, CP Denton Arthritis Res. Ther, 2017;19(1):45. Species: Human Sample Type: Serum Application: ELISA Capture

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Related Product & Information

Background

CCL18/PARC

background_content

Background: CCL18/PARC CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein 4 (MIP-4), and dendritic cell chemokine (DC‑CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10, and IL-13, and inhibited by IFN-gamma. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells but not monocytes or neutrophils.

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Citations

1. Multiplex serum protein analysis reveals potential mechanisms and markers of response to hyperimmune caprine serum in systemic sclerosis
   Authors: N Quillinan, KE Clark, B Youl, J Vernes, D McIntosh, S Haq, CP Denton
   Arthritis Res. Ther, 2017;19(1):45.
   Species: Human
   Sample Type: Serum
   Application: ELISA Capture


2. Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer
   Authors: S Su, J Liao, J Liu, D Huang, C He, F Chen, L Yang, W Wu, J Chen, L Lin, Y Zeng, N Ouyang, X Cui, H Yao, F Su, JD Huang, J Lieberman, Q Liu, E Song
   Cell Res, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
   Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
   PLoS ONE, 2017;12(4):e0175712.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


4. Epstein-Barr virus-induced VEGF and GM-CSF drive nasopharyngeal carcinoma metastasis via recruitment and activation of macrophages
   Authors: D Huang, S Song, ZZ Wu, W Wu, X Cui, JN Chen, MS Zeng, S Su
   Cancer Res., 2017;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


5. Osseous manifestations of adult Gaucher disease in the era of enzyme replacement therapy.
   Authors: Deegan PB, Pavlova E, Tindall J
   Medicine (Baltimore), 2011;90(1):52-60.
   Species: Human
   Sample Type: Plasma
   Application: Luminex Assay Development


6. Potential biomarkers of osteonecrosis in Gaucher disease.
   Authors: Pavlova EV, Deegan PB, Tindall J, McFarlane I, Mehta A, Hughes D, Wraith JE, Cox TM
   Blood Cells Mol. Dis., 2011;46(1):27-33.
   Species: Human
   Sample Type: Serum
   Application: Luminex Development


7. The dendritic cell-specific chemokine, dendritic cell-derived CC chemokine 1, enhances protective cell-mediated immunity to murine malaria.
   Authors: Bruna-Romero O, Schmieg J, Del Val M, Buschle M, Tsuji M
   J. Immunol., 2003;170(6):3195-203.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC

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