抗HCC-4抗体(Anti-HCC-4, Human, Mouse-Mono antibody)
掲載日情報：2021/01/28 現在Webページ番号：28287
HCC-4に対する抗体(Anti-HCC-4, Human, Mouse-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
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Anti-HCC-4, Human, Mouse-Mono(70218.11) |
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Anti-Human CCL16/HCC-4 MAb (Clone 70218) |
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[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-HCC-4, Human, Mouse-Mono(70218.11)
文献数： 1
- 商品コード：MAB328
- メーカー：RSD
- 包装：500μg
- 価格：¥121,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human CCL16/HCC-4 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF802，スタンダードとして#802-HC-025を用いる。 別名：C-C motif chemokine 16 クローン：70218 Genbank No： 6360 Protein Accession No： O15467 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Mouse | ||||
| 交差性 | Human | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human CCL16/HCC-4 MAb (Clone 70218)
文献数： 0
- 商品コード：MAB328-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：C-C motif chemokine 16 クローン：70218 Genbank No： 6360 Protein Accession No： O15467 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | Human | 免疫動物 | Mouse | ||||
| 交差性 | Human | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human CCL16/HCC-4Gln24-Gln120Accession # O15467 |
| Source | Monoclonal Mouse IgG1 Clone # 70218 |
| Purification | Protein A or G purified from ascites |
| Specificity | Detects human CCL16/HCC‑4 in direct ELISAs and Western blots. |
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Applications and Data
| Recommended Concentration | Sample | |
| Human CCL16/HCC-4 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human CCL16/HCC‑4 Antibody (Catalog #MAB328 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human CCL16/HCC‑4 Biotinylated Antibody (Catalog #BAF802 ) |
| ELISA Standard | Recombinant Human CCL16/HCC-4 Protein (Catalog #802-HC ) | |
| Neutralization | Measured by its ability to neutralize CCL16/HCC‑4-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 0.75 µg/mL Recombinant Human CCL16/HCC‑4. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
![Neutralization CCL16/HCC-4/LEC Antibody (70218) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/CCL16_MAB328_Block_Neutralize_9150.jpg) click image to view larger | Chemotaxis Induced by CCL16/HCC‑4 and Neutralization by Human CCL16/ HCC‑4 Antibody. Recombinant Human CCL16/ HCC‑4 (Catalog # 802‑HC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL16/ HCC‑4 (0.75 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL16/ HCC‑4 Monoclonal Antibody (Catalog # MAB328). The ND50 is typically 5‑20 µg/mL. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CCL16/HCC-4 | Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 cDNA encodes a 120 amino acid (aa) residue precursor protein with a 23 aa residue predicted signal peptide that is cleaved to generate a 97 aa residue mature protein. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% aa sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1. Two potential polyadenylation signals are present on the human HCC-4 gene, and as a result, two transcripts containing approximately 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, gamma δ T cells, and some T cell clones. The expression of HCC-4 in monocytes is highly upregulated in the presence of IL-10. The HCC-4 gene has been mapped to chromosome 17q where multiple CC chemokines are clustered. Recombinant HCC-4 has been shown to chemoattract human monocytes and THP-1 cells but not resting lymphocytes or neutrophils. HCC-4 has also been found to suppress proliferation of myeloid progenitor cells. The HCC-4 induced calcium flux in THP-1 cells can be desensitized by prior exposure to RANTES, suggesting that HCC-4 and RANTES share the same receptor in THP-1 cells. |
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References
| Shoudai, K. et al. (1998) Biochim. Biophys. Acta 1396:273. | |
| Hedrick, J. et al. (1998) Blood 91:4242. | |
| Youn, B-S. et al. (1998) BBRC 247:217. | |
| Entrez Gene IDs: | 6360 (Human) |
| Alternate Names: | C-C motif chemokine 16; CCL16; chemokine (C-C motif) ligand 16; Chemokine CC-4; Chemokine LEC; CKb12; HCC4; HCC-4; HCC-4Liver-expressed chemokine; LCC-1Lymphocyte and monocyte chemoattractant; LEC; liver CC chemokine-1; LMC; LMCSmall-inducible cytokine A16; Monotactin-1; Mtn-1; NCC-4; NCC4IL-10-inducible chemokine; NCC-4ILINCK; new CC chemokine 4; SCYA16MGC117051; SCYL4; small inducible cytokine subfamily A (Cys-Cys), member 16 |
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Related Research Areas
| CC Chemokines and Receptors | ||
| Chemokines and Receptors | ||
| Chemokines and Receptors in Angiogenesis | ||
| Macrophage Activation Markers | ||
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| Neutralization | |
|---|---|
 | Chemotaxis Induced by CCL16/HCC‑4 and Neutralization by Human CCL16/HCC‑4 Antibody. Recombinant Human CCL16/HCC‑4 (Catalog # 802‑HC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL16/HCC‑4 (0.75 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL16/HCC‑4 Monoclonal Antibody (Catalog # MAB328). The ND50 is typically 5‑20 µg/mL. |
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Related Product & Information
| Entrez Gene IDs | 6360 (Human) |
|---|---|
| Background | CCL16/HCC-4 |
| background_content | Background: CCL16/HCC-4 Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 cDNA encodes a 120 amino acid (aa) residue precursor protein with a 23 aa residue predicted signal peptide that is cleaved to generate a 97 aa residue mature protein. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% aa sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1. Two potential polyadenylation signals are present on the human HCC-4 gene, and as a result, two transcripts containing approximately 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, gamma δ T cells, and some T cell clones. The expression of HCC-4 in monocytes is highly upregulated in the presence of IL-10. The HCC-4 gene has been mapped to chromosome 17q where multiple CC chemokines are clustered. Recombinant HCC-4 has been shown to chemoattract human monocytes and THP-1 cells but not resting lymphocytes or neutrophils. HCC-4 has also been found to suppress proliferation of myeloid progenitor cells. The HCC-4 induced calcium flux in THP-1 cells can be desensitized by prior exposure to RANTES, suggesting that HCC-4 and RANTES share the same receptor in THP-1 cells. |
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Citations
- CCL16/LEC powerfully triggers effector and antigen-presenting functions of macrophages and enhances T cell cytotoxicity.
Authors: Cappello P, Caorsi C, Bosticardo M
J. Leukoc. Biol., 2004;75(1):135-42.
Species: Mouse
Sample Type: Whole Cells
Application: Neut
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