抗Human IL-12 p70抗体(Anti-Human IL-12 p70 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-12 p70
Source	Monoclonal Mouse IgG1 Clone # 24910
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-12 p70 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IL-12 p40 homodimer or rhIL-23 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL‑12 (Catalog # 219-IL) under non-reducing conditions only
Neutralization	Measured by its ability to neutralize IL‑12 p70-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). Stern, A. S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:6808. The Neutralization Dose (ND50) is typically 0.3-0.9 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑12.

Neutralization

Cell Proliferation Induced by IL‑12 and Neutralization by Human IL‑12 p70 Antibody. Recombinant Human IL‑12 (Catalog # 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑12 p70 Monoclonal Antibody (Catalog # MAB219). The ND50 is typically 0.3-0.9 µg/mL.

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Related Product & Information

Long Name	Interleukin 12 p70
Entrez Gene IDs	3592 (Human); 16159 (Mouse); 84405 (Rat)
Background	IL-12 p70
background_content	Background: IL-12 p70 Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. Biologically active IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit.The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. Current evidence indicates that IL-12, produced by macrophages in response to infectious agents, is a central mediator of the cell-mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.

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Citations

1. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling
   Sci Rep, 2016;6(0):32320.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Bacillus Calmette-Gu�rin (BCG) Revaccination of Adults with Latent Mycobacterium tuberculosis Infection Induces Long-Lived BCG-Reactive NK Cell Responses
   J Immunol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes
   Authors: RB Jones, S Mueller, R O'Connor, K Rimpel, DD Sloan, D Karel, HC Wong, EK Jeng, AS Thomas, JB Whitney, SY Lim, C Kovacs, E Benko, S Karandish, SH Huang, MJ Buzon, M Lichterfel, A Irrinki, JP Murry, A Tsai, H Yu, R Geleziunas, A Trocha, MA Ostrowski, Irvi
   PLoS Pathog, 2016;12(4):e1005545.
   Species: Human
   Sample Type: Whole Cells
   Application: Bioassay


4. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs
   Authors: SM Bal, JH Bernink, M Nagasawa, J Groot, MM Shikhagaie, K Golebski, CM van Drunen, R Lutter, RE Jonkers, P Hombrink, M Bruchard, J Villaudy, JM Munneke, W Fokkens, JS Erjefält, H Spits, XR Ros
   Nat Immunol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4.
   Authors: Ranzani, Valeria, Rossetti, Grazisa, Panzeri, Ilaria, Arrigoni, Alberto, Bonnal, Raoul J, Curti, Serena, Gruarin, Paola, Provasi, Elena, Sugliano, Elisa, Marconi, Maurizio, De Francesco, Raffaele, Geginat, Jens, Bodega, Beatrice, Abrignani, Sergio, Pagani, Massimil
   Nat Immunol, 2015;16(3):318-25.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Toll-like receptor 8 agonist and bacteria trigger potent activation of innate immune cells in human liver.
   Authors: Jo J, Tan A, Ussher J, Sandalova E, Tang X, Tan-Garcia A, To N, Hong M, Chia A, Gill U, Kennedy P, Tan K, Lee K, De Libero G, Gehring A, Willberg C, Klenerman P, Bertoletti A
   PLoS Pathog, 2014;10(6):e1004210.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. IL-1 Family Members IL-18 and IL-33 Upregulate the Inflammatory Potential of Differentiated Human Th1 and Th2 Cultures.
   Authors: Blom L, Poulsen L
   J Immunol, 2012;189(9):4331-7.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
   Authors: Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S
   J. Immunol., 2009;183(12):8176-85.
   Species: Human
   Sample Type: Whole Cells
   Application: Bioassay


9. Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus.
   Authors: Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V
   J. Exp. Med., 2009;206(4):793-805.
   Species: Human
   Sample Type: Whole Cells
   Application: Blocking


10. Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay.
    Authors: Miller R, Sadhukhan R, Wu C
    J. Immunol. Methods, 2008;334(1):134-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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