抗Human IL-1 beta/IL-1F2抗体(Anti-Human IL-1 beta/IL-1F2 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL-1 beta /IL-1F2aa 117-269Accession # P01584
Source	Monoclonal Mouse IgG1 Clone # 8516
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-1 beta /IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL‑1 beta and rpIL‑1 beta and no cross-reactivity with rrIL‑1 beta, rmIL‑1 alpha, rhIL‑1ra, rmIL‑1ra, or rrIL‑1 alpha.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	Human peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Neutralization	Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.001-0.003 µg/mL in the presence of 50 pg/mL Recombinant Human IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.

Neutralization
	Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by HumanIL‑1 beta /IL‑1F2 Antibody. Recombinant HumanIL‑1 beta /IL‑1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201). The ND50 is typically 0.001-0.003 µg/mL in the presence of concanavalin A (1.25 µg/mL).
Western Blot
	Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	IL‑1 beta /IL‑1F2 in Human PBMCs. IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunocytochemistry
	IL‑1 beta /IL‑1F2 in Human PBMCs. IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Long Name	Interleukin 1 beta
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Background	IL-1 beta/IL-1F2
background_content	Background: IL-1 beta/IL-1F2 IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

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Citations

1. Extracellular Neutrophil Proteases Are Efficient Regulators of IL-1, IL-33, and IL-36 Cytokine Activity but Poor Effectors of Microbial Killing
   Authors: DM Clancy, GP Sullivan, HBT Moran, CM Henry, EP Reeves, NG McElvaney, EC Lavelle, SJ Martin
   Cell Rep, 2018;22(11):2937-2950.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Human Scavenger Receptor A1-Mediated Inflammatory Response to Silica Particle Exposure Is Size Specific
   Authors: N Nishijima, T Hirai, K Misato, M Aoyama, E Kuroda, KJ Ishii, K Higashisak, Y Yoshioka, Y Tsutsumi
   Front Immunol, 2017;8(0):379.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


3. Adipose progenitor cell secretion of GM-CSF and MMP9 promotes a stromal and immunological microenvironment that supports breast cancer progression
   Authors: F Reggiani, V Labanca, P Mancuso, C Rabascio, G Talarico, S Orecchioni, A Manconi, F Bertolini
   Cancer Res., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Opposing effects of Nrf2 and Nrf2-activating compounds on the NLRP3 inflammasome independent of Nrf2-mediated gene expression
   Authors: M Garstkiewi, GE Strittmatt, S Grossi, J Sand, G Fenini, S Werner, LE French, HD Beer
   Eur. J. Immunol, 2017;0(0):.
   


5. Processing and secretion of guanylate binding protein-1 depend on inflammatory caspase activity
   Authors: E Naschberge, W Gei beta dörfer, C Bogdan, P Tripal, E Kremmer, M Stürzl, N Britzen-La
   J. Cell. Mol. Med, 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Downregulation of estrogen receptor and modulation of growth of breast cancer cell lines mediated by paracrine stromal cell signals
   Authors: R A Steinman
   Breast Cancer Res. Treat., 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses
   Authors: Wan-Chien Cheng
   Eur J Immunol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Differential IL-1? secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability
   Sci Rep, 2016;6(0):39035.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis
   Arch Med Sci, 2016;12(5):950-958.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


10. Herpesvirus Genome Recognition Induced Acetylation of Nuclear IFI16 Is Essential for Its Cytoplasmic Translocation, Inflammasome and IFN-beta Responses.
    Authors: Ansari M, Dutta S, Veettil M, Dutta D, Iqbal J, Kumar B, Roy A, Chikoti L, Singh V, Chandran B
    PLoS Pathog, 2015;11(7):e1005019.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB

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