抗Siglec-5抗体(Anti-Siglec-5, Mouse-Mono antibody)
掲載日情報:2019/10/21 現在Webページ番号:28127
Siglec-5に対する抗体(Anti-Siglec-5, Mouse-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 :2025年04月26日 20時55分現在]
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Anti-Siglec-5, Mouse-Mono(194117) |
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Anti-Human Siglec-5 MAb (Clone 194117) |
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[在庫・価格 :2025年04月26日 20時55分現在]
Anti-Siglec-5, Mouse-Mono(194117)
文献数: 5
- 商品コード:MAB1072
- メーカー:RSD
- 包装:500μg
- 価格:¥121,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 別名:CD170 クローン:194117 Genbank No: 8778 Protein Accession No: O15389 |
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別包装品 | 別包装品あり | ||||||
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保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Mouse | ||||
交差性 | Human | 適用 | IHC,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | Myeloid-derived Suppressor Cell Marker |
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関連記事 |
Anti-Human Siglec-5 MAb (Clone 194117)
文献数: 1
- 商品コード:MAB1072-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
追加しました。
Product Details
Species Reactivity | Human |
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Label | Unconjugated |
Immunogen | Mouse myeloma cell line NS0-derived recombinant human Siglec‑5/CD170Lys18-Thr434Accession # O15389 |
Source | Monoclonal Mouse IgG2B Clone # 194117 |
Purification | Protein A or G purified from hybridoma culture supernatant |
Specificity | Detects human Siglec‑5/CD170 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Siglec-2, -3, -7, -9, or -14 is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 1 µg/mL | Recombinant Human Siglec‑5 Fc Chimera (Catalog # 1072-SL) |
Immunohistochemistry | 8-25 µg/mL | Immersion fixed paraffin-embedded sections of human spleen |
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Related Product & Information
Background | Siglec-5/CD170 |
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background_content | Background: Siglec-5/CD170 Siglecs (1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins (2) belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding (3), followed by varying numbers of Ig-like C2-type domains (1, 4). Eleven human Siglecs have been cloned and characterized (1, 4). They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and the Siglec-5 to 11 (4, 5, 7). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglec-5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (4, 5). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasma tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system. Human Siglec-5 cDNA encodes a 551 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, three Ig-like C2-type domains, a transmembrane region and a cytoplasma tail (6). Siglec-5 exists as a disulfide-linked homodimer on the cell surface and is expressed on monocytes, neutrophils and B cells (4, 5, 6). It binds equally well to both alpha 2,3- and alpha 2,6-linked sialic acid (6). |
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Citations
- Siglec-5 and Siglec-14 are polymorphic paired receptors that modulate neutrophil and amnion signaling responses to group B Streptococcus.
Authors: Ali S, Fong J, Carlin A, Busch T, Linden R, Angata T, Areschoug T, Parast M, Varki N, Murray J, Nizet V, Varki A
J Exp Med, 2014;211(6):1231-42.
Species: Human
Sample Type: Cell Lysates
Application: WB - Human Siglec-5 inhibitory receptor and immunoglobulin A (IgA) have separate binding sites in streptococcal beta protein.
Authors: Nordstrom T, Movert E, Olin AI, Ali SR, Nizet V, Varki A, Areschoug T
J. Biol. Chem., 2011;286(39):33981-91.
Species: Human
Sample Type: Recombinant Protein
Application: WB - Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates.
Authors: Angata T, Hayakawa T, Yamanaka M, Varki A, Nakamura M
FASEB J., 2006;20(12):1964-73.
Species: Human
Sample Type: Whole Cells
Application: ICC
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