抗Epiregulin抗体(Anti-Epiregulin, Mouse, Rat-Mono antibody)
掲載日情報：2021/01/28 現在Webページ番号：28123
Epiregulinに対する抗体(Anti-Epiregulin, Mouse, Rat-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月29日 20時55分現在]
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Anti-Epiregulin, Mouse, Rat-Mono(189611) |
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Anti-Mouse Epiregulin MAb (Clone 189611) |
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[在庫・価格 ：2025年04月29日 20時55分現在]
Anti-Epiregulin, Mouse, Rat-Mono(189611)
文献数： 1
- 商品コード：MAB1068
- メーカー：RSD
- 包装：500μg
- 価格：¥121,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Mouse Epiregulin サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF1068，スタンダードとして#1068-EP-050を用いる。 別名：epiregulin クローン：189611 Genbank No： 2069 Protein Accession No： Q61521 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Mouse | 免疫動物 | Rat | ||||
| 交差性 | Mouse | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | R&D Systems社 Epiregulin（エピレグリン）関連製品 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Mouse Epiregulin MAb (Clone 189611)
文献数： 0
- 商品コード：MAB1068-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：epiregulin クローン：189611 Genbank No： 2069 Protein Accession No： Q61521 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Rat | |||||
| 交差性 | Mouse | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant mouse EpiregulinVal56-Leu101Accession # Q61521 |
| Source | Monoclonal Rat IgG2A Clone # 189611 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects mouse Epiregulin in ELISAs. In direct ELISAs, 100% cross‑reactivity with recombinant human Epiregulin is observed, but no cross‑reactivity with recombinant mouse (rm) Epigen, recombinant human (rh) TGF-alpha, rhAmphiregulin, rmBetacellulin, rhEGF, rhHB-EGF, rhHRG-alpha, or rhHRG-beta is observed. In sandwich immunoassays, no cross‑reactivity with rmAmphiregulin, rhNRG-1-beta 1, rmBetacellulin, rmEGF, rhHeregulin, rhHB-EGF, or rhTGF‑ alpha is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Mouse Epiregulin Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse Epiregulin Antibody (Catalog #MAB1068 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse Epiregulin Biotinylated Antibody (Catalog #BAF1068 ) |
| ELISA Standard | Recombinant Mouse Epiregulin Protein (Catalog #1068-EP ) | |
| Neutralization | Measured by its ability to neutralize Epiregulin-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 3 ng/mL Recombinant Mouse Epiregulin. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
 click image to view larger | Cell Proliferation Induced by Epiregulin and Neutralization by Mouse Epiregulin Antibody.Recombinant Mouse Epiregulin (Catalog # 1068-EP) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Epiregulin (3 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti‑Mouse Epiregulin Monoclonal Antibody (Catalog # MAB1068). The ND50 is typically 0.2‑1 µg/mL. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: Epiregulin | Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. They are all synthesized as transmembrane precursors and converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7 (1). The mouse Epiregulin cDNA encodes for a 162 amino acid (aa) transmembrane precursor, with the mature soluble form comprising aa 56‑101 (2). The mode of action of Epiregulin is similar to other EGF family members in that it binds to and activates the tyrosine-kinase, ErbB-family receptors (ErbB1 through B4) (3). Although it stimulates phosphorylation of all four receptors, it appears to interact primarily with ErbB1 and ErbB4. Epiregulin has the broadest specificity of the EGF-like ligands but seems to preferentially activate heterodimeric receptor complexes (4). Epiregulin exhibits a variety of biological effects. It was originally shown to both inhibit growth of several epithelial tumor cells and stimulate growth of fibroblasts and other types of cells (1). Epiregulin expression is upregulated in a number of carcinoma cell lines. It has also been shown to be an autocrine growth factor in human epidermal keratinocytes (5). Epiregulin has also been shown to play a role in the early steps of pregnancy, regulating attachment of the blastocyst to the uterine epithelium during the implantation process (6). |
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References
| Toyoda, H. et al. (1995) J. Biol. Chem. 270:7495. | |
| Toyoda, H. et al. (1995) FEBS Lett. 377:403. | |
| Komurasaki, T. et al. (1997) Oncogene 15:2841. | |
| Shelly, M. et al. (1998) J. Biol. Chem. 273:10496. | |
| Shirakata, Y. et al. (2000) J. Biol. Chem. 275:5748. | |
| Das, S.K. et al. (1997) Dev. Biol. 190:178. | |
| Entrez Gene IDs: | 2069 (Human); 13874 (Mouse) |
| Alternate Names: | Epiregulin; ER; EREG; proepiregulin |
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Related Research Areas
| EGF Family | ||
| EGF Ligands | ||
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| Neutralization | |
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 | Cell Proliferation Induced by Epiregulin and Neutralization by Mouse Epiregulin Antibody. Recombinant Mouse Epiregulin (Catalog # 1068-EP) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Epiregulin (3 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti‑Mouse Epiregulin Monoclonal Antibody (Catalog # MAB1068). The ND50 is typically 0.2‑1 µg/mL. |
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Related Product & Information
| Entrez Gene IDs | 2069 (Human); 13874 (Mouse) |
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| Background | Epiregulin |
| background_content | Background: Epiregulin Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. They are all synthesized as transmembrane precursors and converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7 (1). The mouse Epiregulin cDNA encodes for a 162 amino acid (aa) transmembrane precursor, with the mature soluble form comprising aa 56‑101 (2). The mode of action of Epiregulin is similar to other EGF family members in that it binds to and activates the tyrosine-kinase, ErbB-family receptors (ErbB1 through B4) (3). Although it stimulates phosphorylation of all four receptors, it appears to interact primarily with ErbB1 and ErbB4. Epiregulin has the broadest specificity of the EGF-like ligands but seems to preferentially activate heterodimeric receptor complexes (4). Epiregulin exhibits a variety of biological effects. It was originally shown to both inhibit growth of several epithelial tumor cells and stimulate growth of fibroblasts and other types of cells (1). Epiregulin expression is upregulated in a number of carcinoma cell lines. It has also been shown to be an autocrine growth factor in human epidermal keratinocytes (5). Epiregulin has also been shown to play a role in the early steps of pregnancy, regulating attachment of the blastocyst to the uterine epithelium during the implantation process (6). |
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Citations
- Temporal expression of growth factors triggered by epiregulin regulates inflammation development.
Authors: Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M
J Immunol, 2015;194(3):1039-46.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded
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