抗Human IL-21 R Phycoerythrin抗体(Anti-Human IL-21 R Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-21 RCys20-Pro236Accession # Q9HBE5
Source	Monoclonal Mouse IgG1 Clone # 152512
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-21 R in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh)IL‑2 R beta or rmIL‑21 R.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of IL-21 R in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human IL-21 R PE-conjugated Monoclonal Antibody (Catalog # FAB9911P) and Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

IL-21 R

background_content

Background: IL-21 R Interleukin-21 (IL-21) and its receptor play an important role in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta, IL-4 R alpha, and IL-9 R. The gene for human IL-21 R has been mapped to chromosome 16p12. Human IL-21 R is a 538 amino acid (aa) residue type I transmembrane protein with a 19 aa signal peptide, a 217 aa extracellular domain, a 19 aa transmembrane domain, and a 283 aa cytoplasmic domain. IL-21 R is expressed on lymphoid tissues, peripheral B cells, and cell lines of T, B, and NK lineage. The common gamma  chain ( gamma c) is required for IL-21 R signaling. The IL-21/IL-21 R interaction appears to play an important role in B and T cell proliferation after antigen stimulation and NK cell maturation.

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Citations

1. Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
   Authors: Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P
   Immunol. Lett., 2011;134(2):113-21.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. IL-21 Promotes Skin Recruitment of CD4+ Cells and Drives IFN-{gamma}-Dependent Epidermal Hyperplasia.
   Authors: Sarra M, Caruso R, Cupi ML, Monteleone I, Stolfi C, Campione E, Diluvio L, Mazzotta A, Botti E, Chimenti S, Costanzo A, MacDonald TT, Pallone F, Monteleone G
   J. Immunol., 2011;186(9):5435-42.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Interleukin-21 induces the differentiation of human Tc22 cells via phosphorylation of signal transducers and activators of transcription.
   Authors: Liu Y, Yang B, Ma J, Wang H, Huang F, Zhang J, Chen H, Wu C
   Immunology, 2011;132(4):540-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Heterogeneous expression and function of IL-21R and susceptibility to IL-21-mediated apoptosis in follicular lymphoma cells.
   Authors: de Totero D, Capaia M, Fabbi M, Croce M, Meazza R, Cutrona G, Zupo S, Loiacono F, Truini M, Ferrarini M, Ferrini S
   Exp. Hematol., 2010;38(5):373-83.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Regulating human Th17 cells via differential expression of IL-1 receptor.
   Authors: Lee WW, Kang SW, Choi J
   Blood, 2010;115(3):530-40.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Effect of IL-21 on NK cells derived from different umbilical cord blood populations.
   Authors: Perez S, Mahaira L, Sotiropoulou P, Gritzapis A, Iliopoulou E, Niarchos D, Cacoullos N, Kavalakis Y, Antsaklis A, Sotiriadou N, Baxevanis C, Papamichail M
   Int Immunol, 0;18(1):49-58.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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