抗Human VEGF R2/KDR Phycoerythrin抗体(Anti-Human VEGF R2/KDR Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R2/KDR/Flk-1Ala20-Glu764Accession # P35968
Source	Monoclonal Mouse IgG1 Clone # 89106
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human VEGF R2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGF R1, rhVEGF R3 or recombinant mouse VEGF R2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of VEGF R2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R2/KDR/Flk‑1 PE‑conjugated Mono­clonal Antibody (Catalog # FAB357P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

VEGF R2/KDR/Flk-1

background_content

Background: VEGF R2/KDR/Flk-1 VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGF R2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

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Citations

1. Putative mechanisms Underlying Myocardial infarction onset and Emotions (PUME): a randomised controlled study protocol
   Authors: I Ensari, MM Burg, KM Diaz, J Fu, AT Duran, JM Suls, JA Sumner, R Monane, JE Julian, S Zhao, WF Chaplin, D Shimbo
   BMJ Open, 2018;8(5):e020525.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Effect of intensive multifactorial treatment on vascular progenitor cells in hypertensive patients
   Authors: C Maroun-Eid, A Ortega-Her, J Modrego, M Abad-Cardi, JA García-Don, L Reinares, N Martell-Cl, D Gómez-Garr
   PLoS ONE, 2018;13(1):e0190494.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis
   Authors: Y Li, PM Brauer, J Singh, S Xhiku, K Yoganathan, JC Zúñiga-Pfl, MK Anderson
   Stem Cell Reports, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Chromatin and Transcriptional Analysis of Mesoderm Progenitor Cells Identifies HOPX as a Regulator of Primitive Hematopoiesis
   Authors: NJ Palpant, Y Wang, B Hadland, RJ Zaunbreche, M Redd, D Jones, L Pabon, R Jain, J Epstein, WL Ruzzo, Y Zheng, I Bernstein, A Margolin, CE Murry
   Cell Rep, 2017;20(7):1597-1608.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Effects of a New Nutraceutical Formulation (Berberine, Red Yeast Rice and Chitosan) on Non-HDL Cholesterol Levels in Individuals with Dyslipidemia: Results from a Randomized, Double Blind, Placebo-Controlled Study
   Authors: V Spigoni, R Aldigeri, M Antonini, MM Micheli, F Fantuzzi, A Fratter, M Pellizzato, E Derlindati, I Zavaroni, RC Bonadonna, A Dei Cas
   Int J Mol Sci, 2017;18(7):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Increased serum levels of fractalkine and mobilisation of CD34(+)CD45(-) endothelial progenitor cells in systemic sclerosis
   Authors: A Benyamine, J Magalon, S Cointe, R Lacroix, L Arnaud, N Bardin, P Rossi, Y Francès, F Bernard-Gu, G Kaplanski, JR Harlé, PJ Weiller, P Berbis, D Braunstein, E Jouve, N Lesavre, F Couranjou, F Dignat-Geo, F Sabatier, P Paul, B Granel
   Arthritis Res. Ther, 2017;19(1):60.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Hydrogen Sulfide-Preconditioning of Human Endothelial Progenitor Cells Transplantation Improves Re-Endothelialization in Nude Mice with Carotid Artery Injury
   Authors: X Ke, J Zou, Q Hu, X Wang, C Hu, R Yang, J Liang, X Shu, R Nie, C Peng
   Cell. Physiol. Biochem., 2017;43(1):308-319.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Sex Differences in Circulating Progenitor Cells
   Authors: ML Topel, SS Hayek, YA Ko, PB Sandesara, A Samman Tah, I Hesaroieh, E Mahar, GS Martin, EK Waller, AA Quyyumi
   J Am Heart Assoc, 2017;6(10):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. The blebbishield emergency program overrides chromosomal instability and phagocytosis checkpoints in cancer stem cells
   Authors: GG Jinesh, AM Kamat
   Cancer Res., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. VEGF-induced intracellular Ca2+ oscillations are down-regulated and do not stimulate angiogenesis in breast cancer-derived endothelial colony forming cells
    Authors: F Lodola, U Laforenza, F Cattaneo, FA Ruffinatti, V Poletto, M Massa, R Tancredi, E Zuccolo, DA Khdar, A Riccardi, M Biggiogera, V Rosti, G Guerra, F Moccia
    Oncotarget, 2017;8(56):95223-95246.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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