抗DC-SIGN2抗体(Anti-DC-SIGN2, Human, Mouse-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299Accession # Q9H2X3
Source	Monoclonal Mouse IgG2B Clone # 120604
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human DC‑SIGNR/CD299.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of DC‑SIGNR/CD299 in 3T3 Mouse Cell Line Transfected with Human DC-SIGNR/CD299 by Flow Cytometry. 3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGNR/CD299 was stained with Mouse Anti-Human DC‑SIGNR/CD299 PE‑conjugated Monoclonal Antibody (Catalog # FAB162P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

DC-SIGNR/CD299

background_content

Background: DC-SIGNR/CD299 Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1-4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).

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Citations

1. Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes
   Authors: RA Botting, KM Bertram, H Baharlou, KJ Sandgren, J Fletcher, JW Rhodes, H Rana, TM Plasto, XM Wang, JJ Lim, L Barnouti, MP Kohout, T Papadopoul, S Merten, N Olbourne, AL Cunningham, M Haniffa, AN Harman
   J. Leukoc. Biol, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Mechanism and significance of cell type-dependent neutralization of flaviviruses.
   Authors: Mukherjee S, Dowd K, Manhart C, Ledgerwood J, Durbin A, Whitehead S, Pierson T
   J Virol, 2014;88(13):7210-20.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells.
   Authors: Messmer D, Jacque JM, Santisteban C, Bristow C, Han SY, Villamide-Herrera L, Mehlhop E, Marx PA, Steinman RM, Gettie A, Pope M
   J. Immunol., 2002;169(8):4172-82.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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