抗Human DC-SIGN/CD209 Fluorescein抗体(Anti-Human DC-SIGN/CD209 Fluorescein antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Fluorescein
Immunogen	NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGN/CD209
Source	Monoclonal Mouse IgG2B Clone # 120507
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human DC‑SIGN/CD209 on transfected NIH/3T3 cells and on monocyte derived dendritic cells. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of DC‑SIGN/CD209 in NIH‑3T3 Mouse Cell Line Transfected with Human DC-SIGN/CD209 by Flow Cytometry. NIH‑3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGN/CD209 was stained with Mouse Anti-Human DC‑SIGN/CD209 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB161F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

DC-SIGN/CD209

background_content

Background: DC-SIGN/CD209 Human DC-Sign (dendritic cell-specific ICAM-3 grabbing nonintegrin; also known as CD209) is a member of the chromosome 19 C-type lectin family that includes DC-SIGN, DC-SIGN-related protein, CD23 and LSECtin (1). DC-SIGN was initially reported to be a 46 kDa, 404 amino acid (aa) type II transmembrane protein that contained a 40 aa cytoplasmic N-terminus, a 21 aa transmembrane segment, and a 343 aa extracellular C-terminus (2). The extracellular region contains a distal, 115 aa Ca++-dependent carbohydrate-binding lectin domain and a membrane-proximal linker segment that is composed of seven 23 aa repeats (2, 3). The lectin domain is believed to preferably bind mannose, either within the context of ICAM-3 (on T cells) or ICAM-2 (on endothelial cells) (2, 4, 5). DC-SIGN expression appears to be limited to dendritic cells (DC) and macrophages (6), and DC interaction with the ICAMs both aids DC cell trafficking and immunological synapse formation (7). Since the original report on DC-SIGN, multiple splice forms have been discovered, generating both membrane-bound and soluble forms (3). There are eight type A isoforms, all of which begin with the same 15 aa of exon 1a. Four contain the transmembrane region of exon II, and four do not (i.e., are soluble). Among these eight type A isoforms, only three retain the entire 343 aa found in the full length form described in reference #2 (the full length form is referred to as type I mDC-SIGN1A) (3). Five additional isoforms utilize an alternate start site, and these are referred to as type B isoforms. These all show a 35 aa cytoplasmic domain. One also has a transmembrane segment; four do not. Two of the five contain full, unspliced extracellular regions (3). All of this suggests enormous complexity in DC-SIGN biology. DC-SIGN is not well conserved across species. Human and mouse show little overall aa identity. In the lectin domain, however, human DC-SIGN shares 68% aa identity with mouse DC-SIGN (8). Human and rhesus monkey DC-SIGN share 91% aa identity over the entire extracellular region (8). A detailed description of the additional properties of this monoclonal antibody (MAB161) have been published (9, 10).

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Citations

1. DCs facilitate B cell responses against microbial DNA via DC-SIGN
   Authors: JK Sprokholt, MH Heineke, TM Kaptein, JL van Hamme, TBH Geijtenbee
   PLoS ONE, 2017;12(10):e0185580.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Dendritic cells from the human female reproductive tract rapidly capture and respond to HIV
   Mucosal Immunol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Antibody-opsonized bacteria evoke an inflammatory dendritic cell phenotype and polyfunctional Th cells by cross-talk between TLRs and FcRs.
   Authors: Bakema J, Tuk C, van Vliet S, Bruijns S, Vos J, Letsiou S, Dijkstra C, van Kooyk Y, Brenkman A, van Egmond M
   J Immunol, 2015;194(4):1856-66.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Protective effects of astaxanthin on ConA-induced autoimmune hepatitis by the JNK/p-JNK pathway-mediated inhibition of autophagy and apoptosis.
   Authors: Li J, Xia Y, Liu T, Wang J, Dai W, Wang F, Zheng Y, Chen K, Li S, Abudumijiti H, Zhou Z, Wang J, Lu W, Zhu R, Yang J, Zhang H, Yin Q, Wang C, Zhou Y, Lu J, Zhou Y, Guo C
   PLoS ONE, 2015;10(3):e0120440.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Langerhans Cell-Dendritic Cell Cross-Talk via Langerin and Hyaluronic Acid Mediates Antigen Transfer and Cross-Presentation of HIV-1.
   Authors: van den Berg L, Cardinaud S, van der Aar A, Sprokholt J, de Jong M, Zijlstra-Willems E, Moris A, Geijtenbeek T
   J Immunol, 2015;195(4):1763-73.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Epitope specificity and clonality of EBV-specific CTLs used to treat posttransplant lymphoproliferative disease.
   Authors: McAulay KA, Haque T, Urquhart G, Bellamy C, Guiretti D, Crawford DH
   J. Immunol., 2009;182(6):3892-901.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs.
   Authors: Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Munz C
   Proc. Natl. Acad. Sci. U.S.A., 2004;101(47):16606-11.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Involvement of macrophage mannose receptor in the binding and transmission of HIV by macrophages.
   Authors: Nguyen DG, Hildreth JE
   Eur. J. Immunol., 2003;33(2):483-93.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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