抗GM-CSF抗体(Anti-GM-CSF, Feline, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

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Product Details

Species Reactivity	Feline
Label	Biotin
Immunogen	E. coli-derived recombinant feline GM-CSFAla18-Lys144 (Met36Ile, Thr56Ala & Lys126Asn)Accession # AAC06041
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects feline GM-CSF in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse GM-CSF and recombinant rat GM-CSF is observed and 25% cross-reactivity with recombinant human GM-CSF is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Feline GM-CSF (Catalog # 987-FL)

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Related Product & Information

Background

GM-CSF

background_content

Background: GM-CSF GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3‑5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices (8‑10). Mature feline GM-CSF shares 52%‑56% amino acid sequence identity with mouse and rat GM-CSF and 67%‑72% canine, human, and porcine GM‑CSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta  chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (13). Feline and human GM-CSF show cross-species activity (14, 15).

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