抗MIP-3β抗体(Anti-MIP-3β, Mouse, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27965
MIP-3βに対する抗体(Anti-MIP-3β, Mouse, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時35分現在]
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[在庫・価格 ：2025年04月26日 20時35分現在]
Anti-MIP-3β, Mouse, Goat-Poly, Biotin
文献数： 4
- 商品コード：BAF880
- メーカー：RSD
- 包装：50μg
- 価格：¥118,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Mouse CCL19/MIP-3β サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB880-500，スタンダードとして#440-M3-025を用いる。 別名：beta chemokine exodus-3 Genbank No： 6363 Protein Accession No： Q548P0 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Mouse | 免疫動物 | Goat |
| 交差性 | Mouse | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
|---|---|
| Label | Biotin |
| Immunogen | E. coli-derived recombinant mouse CCL19/MIP-3 beta (R&D Systems, Catalog # 440-M3)Gly26-Val107Accession # Q548P0 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse CCL19/MIP-3 beta in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) TECK, rmTARC, recombinant human MIP-3 beta, rmMIP-1 alpha, rmMIP-1 beta, rm6Ckine, and rmRANTES is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse CCL19/MIP‑3 beta (Catalog # 440-M3) |
| Mouse CCL19/MIP-3 beta Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse CCL19/MIP‑3 beta Antibody (Catalog #MAB880 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse CCL19/MIP‑3 beta Biotinylated Antibody (Catalog #BAF880 ) |
| ELISA Standard | Recombinant Mouse CCL19/MIP-3 beta Protein (Catalog #440-M3 ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CCL19/MIP-3 beta | CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a reported beta chemokine that binds specifically to the chemokine receptor CCR-7/EBI-1/BLR-2. Mouse MIP-3 beta cDNA encodes a 108 amino acid residue precursor protein with a predicted 25 aa residue signal peptide that is cleaved to form the 83 aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T cell zone. The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. The MIP-3 beta receptor (CCR7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up-regulated in B-cells infected with Epstein-Barr virus and T cells infected with herpes virus 6 or 7. |
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References
| Kim, C.H. et al. (1998) J. Immunol. 160:2418. | |
| Ngo, V.N. et al. (1998) J. Exp. Med. 188:181. | |
| Rossi, D.L. et al. (1997) J. Immunol. 158:1033. | |
| Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803. | |
| Entrez Gene IDs: | 6363 (Human); 24047 (Mouse); 362506 (Rat) |
| Alternate Names: | beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19 |
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Related Research Areas
| B Cell Chemotaxis, Migration, and Adhesion | ||
| CC Chemokines and Receptors | ||
| Chemokines and Receptors | ||
| Chemokines and Receptors in Angiogenesis | ||
| Chemokines and Receptors in Tfh Cell Differentiation and Function | ||
| Macrophage Activation Markers | ||
| Molecules Secreted by VSMC | ||
| Regulatory T Cells (Tregs) | ||
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Related Product & Information
| Background | CCL19/MIP-3 beta |
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| background_content | Background: CCL19/MIP-3 beta CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a reported beta chemokine that binds specifically to the chemokine receptor CCR-7/EBI-1/BLR-2. Mouse MIP-3 beta cDNA encodes a 108 amino acid residue precursor protein with a predicted 25 aa residue signal peptide that is cleaved to form the 83 aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T cell zone. The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. The MIP-3 beta receptor (CCR7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up-regulated in B-cells infected with Epstein-Barr virus and T cells infected with herpes virus 6 or 7. |
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Citations
- Colonic patch and colonic SILT development are independent and differentially regulated events.
Authors: Baptista A, Olivier B, Goverse G, Greuter M, Knippenberg M, Kusser K, Domingues R, Veiga-Fernandes H, Luster A, Lugering A, Randall T, Cupedo T, Mebius R
Mucosal Immunol, 2013;6(3):511-21.
Species: Mouse
Sample Type: Whole Tissue
Application: IF - The chemokine receptor CCR7 mediates corneal antigen-presenting cell trafficking.
Authors: Jin Y, Shen L, Chong EM
Mol. Vis., 2007;13(0):626-34.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Frozen
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