抗TCA-3抗体(Anti-TCA-3, Mouse, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse CCL1/I-309/TCA-3Lys24-Cys92Accession # P10146
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CCL1/I-309/TCA-3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human I-309, recombinant mouse (rm) Eotaxin-2, rmTARC, and rmMIP-3 beta is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CCL1/I‑309/TCA‑3 (Catalog # 845-TC)
Mouse CCL1/TCA-3 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse CCL1/I‑309/TCA‑3 Antibody (Catalog #MAB8451 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CCL1/I‑309/TCA‑3 Biotinylated Antibody (Catalog #BAF845 )
ELISA Standard		Recombinant Mouse CCL1/TCA-3 Protein (Catalog #845-TC )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL1/I-309/TCA-3	Mouse TCA-3 is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines. TCA-3 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA-3 contains one potential N-linked glycosylation site. Mouse TCA-3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta, and JE. TCA‑3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA-3 has been shown to chemoattract T-cells.

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References

Goya, I. et al. (1998) J. Immunol. 160:1975.
Burd, P. et al. (1987) J. Immunol. 139:3126.
Entrez Gene IDs:	6346 (Human); 20290 (Mouse)
Alternate Names:	C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3

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Related Research Areas

Show

CC Chemokines and Receptors
Chemokines and Receptors
Chemokines and Receptors in Allergy and Asthma
Chemokines and Receptors in Angiogenesis
Macrophage Activation Markers
Regulatory T Cells (Tregs)
Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Very Good		ELISA	Mouse	Anonymous	01/05/2018			Image Details	ELISA: Mouse CCL1/I‑309/TCA‑3 Biotinylated Antibody [BAF845].

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Summary

Application	ELISA
Sample Tested	Serum and Plasma
Species	Mouse
Other Experimental Details	This antibody was used as the detection molecule in an ELISA with MAB8451 as the solid phase molecule.

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Related Product & Information

Background

CCL1/I-309/TCA-3

background_content

Background: CCL1/I-309/TCA-3 Mouse TCA-3 is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines. TCA-3 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA-3 contains one potential N-linked glycosylation site. Mouse TCA-3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta, and JE. TCA‑3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA-3 has been shown to chemoattract T-cells.

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Citations

1. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


2. Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
   Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
   Am. J. Respir. Crit. Care Med., 2009;179(9):772-81.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


3. CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling.
   Authors: Kearley J, Robinson DS, Lloyd CM
   J. Allergy Clin. Immunol., 2008;122(3):617-24.e6.
   Species: Mouse
   Sample Type: BALF
   Application: ELISA Development


4. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
   Authors: Mosmann T
   Eur. J. Immunol., 2004;34(6):1617-26.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


5. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
   Authors: Yang L, Mosmann T
   Eur. J. Immunol., 2004;34(6):1617-26.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development

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