抗PAI-1抗体(Anti-PAI-1, Human, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27914
PAI-1に対する抗体(Anti-PAI-1, Human, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月23日 14時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-PAI-1, Human, Goat-Poly, Biotin <Anti-Serpin E1> |
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[在庫・価格 ：2024年05月23日 14時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-PAI-1, Human, Goat-Poly, Biotin <Anti-Serpin E1>
文献数： 1
- 商品コード：BAF1786
- メーカー：RSD
- 包装：50μg
- 価格：¥118,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Human Serpin E1/PAI-1 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB1786-500，スタンダードとして#1786-PI-010を用いる。 別名：Endothelial plasminogen activator inhibitor Genbank No： 5054 Protein Accession No： P05121 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Goat |
| 交差性 | Human | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 |
Quantikine Human / Total Human Serpin E1 (PAI-1) ELISA Kit |
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| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Biotin |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin E1/PAI-1 (R&D Systems, Catalog # 1786-PI)Ser22-Pro402Accession # P05121 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human Serpin E1/PAI-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin A5, and rhSerpin F2 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | See below |
| Human Serpin E1/PAI-1 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human Serpin E1/PAI‑1 Antibody (Catalog #MAB1786 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human Serpin E1/PAI‑1 Biotinylated Antibody (Catalog #BAF1786 ) |
| ELISA Standard | Recombinant Human Serpin E1/PAI-1 Protein, CF (Catalog #1786-PI ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Western Blot |
![Western Blot Serpin E1/PAI-1 Antibody [Biotin]](http://resources.rndsystems.com/images/datasheets/antibody/Serpin_E1_BAF1786_Western_Blot_19974.jpg) click image to view larger | Detection of Human Serpin E1/PAI‑1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin E1/PAI‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1786) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. A specific band was detected for Serpin E1/PAI‑1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: Serpin E1/PAI-1 | As a member of the Serpin superfamily of serine protease inhibitors, Serpin E1/PAI-1 is the principal inhibitor of urokinase-type plasminogen activator (uPA) and tissue-type PA (1, 2). As important regulators of extracellular matrix remodeling, uPA and PAI-1 play a major role in many processes such as angiogenesis, tumor invasion and obesity (2-4). For example, uPA and PAI-1 are the only tumor prognostic factors validated at the highest level of evidence with regard to their clinical utility in breast cancer (5). The human PAI-1 is initially synthesized as 402 amino acid precursor with a N-terminal signal peptide (6, 7). PAI-1 may exist in one of two possible conformations, designated as active or latent (8). The purified recombinant human (rh) PAI-1 is active against rhuPA. The heterogeneity at the N-terminus of the purified rhPAI-1 has been observed before for both the recombinant and native proteins (9). | |
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References
| Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293. | |
| Stefansson, S. et al. (2003) Curr. Pharm. Des. 9:1545. | |
| Duffy, M.J. (2002) Clin. Chem. 48:1194. | |
| Juhan-Vague, I. et al. (2003) J. Thromb. Haemost. 1:1575. | |
| Harbeck, N. et al. (2002) Clin. Breast Cancer 3:196. | |
| Pannekoek, H. et al. (1986) EMBO J. 5:2539. | |
| Ginsburg, D. et al. (1986) J. Clin. Invest. 78:1673. | |
| Wang, Z. et al. (1996) Biochemistry 35:16443. | |
| Stromqvist, M. et al. (1994) Protein Expr. Purif. 5:309. | |
| Long Name: | Plasminogen Activator Inhibitor |
| Entrez Gene IDs: | 5054 (Human); 18787 (Mouse) |
| Alternate Names: | Endothelial plasminogen activator inhibitor; Nexin; PAI1; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1 |
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Related Research Areas
| Adipocytokines | ||
| Alpha Granule Soluble Molecules | ||
| Cancer Biomarkers | ||
| Coagulation | ||
| Complement Pathway | ||
| DNA Damage and Repair | ||
| Enzymes Secreted by Endothelial Cells | ||
| Mesenchymal Cells and EMT | ||
| Plasmin/Plasminogen and Kallikrein/Kinin Systems | ||
| Serine Proteases and Regulators | ||
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| Western Blot | |
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 | Detection of Human Serpin E1/PAI‑1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Serpin E1/PAI‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1786) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. A specific band was detected for Serpin E1/PAI‑1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions. |
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Related Product & Information
| Background | Serpin E1/PAI-1 |
|---|---|
| background_content | Background: Serpin E1/PAI-1 As a member of the Serpin superfamily of serine protease inhibitors, Serpin E1/PAI-1 is the principal inhibitor of urokinase-type plasminogen activator (uPA) and tissue-type PA (1, 2). As important regulators of extracellular matrix remodeling, uPA and PAI-1 play a major role in many processes such as angiogenesis, tumor invasion and obesity (2-4). For example, uPA and PAI-1 are the only tumor prognostic factors validated at the highest level of evidence with regard to their clinical utility in breast cancer (5). The human PAI-1 is initially synthesized as 402 amino acid precursor with a N-terminal signal peptide (6, 7). PAI-1 may exist in one of two possible conformations, designated as active or latent (8). The purified recombinant human (rh) PAI-1 is active against rhuPA. The heterogeneity at the N-terminus of the purified rhPAI-1 has been observed before for both the recombinant and native proteins (9). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
- A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010;56(0):1320.
Species: Human
Sample Type: Cell Culture Supernates
Application: Luminex Development
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