抗TNF Receptor Ⅱ/TNFRSF1B抗体(Anti-TNF Receptor Ⅱ/TNFRSF1B, Soluble, Human, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	E. coli-derived recombinant human sTNF RII/TNFRSF1B
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human TNF RII/TNFRSF1B in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human TNF RI, recombinant mouse (rm) TNF RI, and rmTNF RII is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human TNF RII/TNFRSF1B Fc Chimera (Catalog # 726-R2)
Human TNF RII/TNFRSF1B Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human TNF RII/TNFRSF1B Antibody (Catalog #MAB726 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human TNF RII/TNFRSF1B Biotinylated Antibody (Catalog #BAF726 )
ELISA Standard		Recombinant Human TNF RII/TNFRSF1B (aa 24-206) Protein (Catalog #1089-R2 )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RII/TNFRSF1B	TNF RII, also known as p75 or p80 TNF R, is a prototypic member of theTNF receptor superfamily and has been designated TNFRSF1B. It binds TNFwith high affinity and is expressed primarily in cells of the immunesystem.
Long Name:	Tumor Necrosis Factor Receptor II
Entrez Gene IDs:	7133 (Human); 21938 (Mouse); 156767 (Rat)
Alternate Names:	CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II

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Related Research Areas

Show

Adipocytokines
Cell Structure and Signaling Molecules
Co-stimulatory and Co-inhibitory Molecules
Cytokines and Receptors in Allergy and Asthma
Cytokines and Receptors in Angiogenesis
Diabetes
Diabetic Peripheral Neuropathy
Endothelial Cell Markers
Neuroinflammation
Obesity
Platelet Cytokine and Growth Factor Receptors
Regulation of Apoptosis by TNF Superfamily Members
Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members
Regulation of T Cell Co-stimulation by TNF Superfamily Members
Regulatory T Cells (Tregs)
T Cell Cytokine Signaling
TNF Superfamily
TNF Superfamily Receptors
Aminopeptidase P3, a new member of the TNF-TNFR2 signaling complex, induces phosphorylation of JNK1 and JNK2. Authors: Inoue M, Kamada H, Abe Y, Higashisaka K, Nagano K, Mukai Y, Yoshioka Y, Tsutsumi Y, Tsunoda S J Cell Sci, 2015;128(4):656-69. Species: Human Sample Type: Cell Lysates Application: WB
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Excellent		ELISA	Human	Anonymous	11/22/2017			Image Details	ELISA: Human TNF RII/TNFRSF1B Biotinylated Antibody [BAF726].

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Summary

Application	ELISA
Sample Tested	Serum and Plasma,Cell culture supernatant
Species	Human
Other Experimental Details	We used MAB726 as the solid phase antibody, 1089-R2 as the immunoassay standard and BAF225 as the detection antibody in a sandwich ELISA. Everything worked very well.

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Related Product & Information

Background	TNF RII/TNFRSF1B
background_content	Background: TNF RII/TNFRSF1B TNF RII, also known as p75 or p80 TNF R, is a prototypic member of the TNF receptor superfamily and has been designated TNFRSF1B. It binds TNF with high affinity and is expressed primarily in cells of the immune system.

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Citations

1. Aminopeptidase P3, a new member of the TNF-TNFR2 signaling complex, induces phosphorylation of JNK1 and JNK2.
   Authors: Inoue M, Kamada H, Abe Y, Higashisaka K, Nagano K, Mukai Y, Yoshioka Y, Tsutsumi Y, Tsunoda S
   J Cell Sci, 2015;128(4):656-69.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
   Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
   Transplantation, 2009;87(12):1814-20.
   Species: Human
   Sample Type: Urine
   Application: Antibody Array Development


3. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
   Authors: Copeland S, Siddiqui J, Remick D
   J. Immunol. Methods, 2004;284(1):99-106.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Array Development


4. A pilot study of pentoxifylline for the treatment of primary sclerosing cholangitis.
   Authors: Bharucha AE, Jorgensen R, Lichtman SN, LaRusso NF, Lindor KD
   Am. J. Gastroenterol., 2000;95(9):2338-42.
   Species: Human
   Sample Type: Serum
   Application: ELISA Development


5. Innate immunity associated with inflammatory responses and cytokine production against common dietary proteins in patients with autism spectrum disorder.
   Authors: Jyonouchi H, Sun S, Itokazu N
   Neuropsychobiology, 0;46(2):76-84.
   Species: Human
   Sample Type: Whole Cells
   Application: ELISA Development

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