抗ICAM-1抗体(Anti-ICAM-1, Human, Sheep-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	Chinese hamster ovary cell line CHO-derived recombinant human ICAM-1Extracellular domain
Source	Polyclonal Sheep IgG
Purification	Antigen Affinity-purified
Specificity	Detects human ICAM-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity with rhICAM-2 and rhICAM-3 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human ICAM‑1/CD54 (Catalog # ADP4)
Human ICAM-1/CD54 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human ICAM‑1/CD54 Antibody (Catalog #MAB720 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human ICAM‑1/CD54 Biotinylated Antibody (Catalog #BAF720 )
ELISA Standard		Recombinant Human ICAM-1/CD54 Protein, CF (Catalog #ADP4 )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-1/CD54	Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.
Long Name:	Intercellular Adhesion Molecule 1
Entrez Gene IDs:	3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names:	BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

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Related Research Areas

Adhesion Molecules

Adhesion Molecules in Angiogenesis

Adhesion Molecules on Endothelial Cells

Adhesion Molecules on VSMC

Adhesive Interactions in Allergy and Asthma

Adipose-derived Stem Cell Markers

B Cell Chemotaxis, Migration, and Adhesion

Dendritic Cell Adhesion and Migration

Endothelial Cell Markers

Immunoglobulin Superfamily CAMs

Mesenchymal Stem Cell Markers

Monocyte Markers

Myeloid-derived Suppressor Cells (MDSC)

Neuroinflammation

Platelet Adhesion Molecules

Regulatory T Cells (Tregs)

T Cell Migration/Adhesion

High plasma levels of soluble intercellular adhesion molecule (ICAM)-1 are associated with cerebral malaria. Authors: Adukpo, Selorme, Kusi, Kwadwo A, Ofori, Michael, Tetteh, John K A, Amoako-Sakyi, Daniel, Goka, Bamenla, Adjei, George O, Edoh, Dominic, Akanmori, Bartholo, Gyan, Ben A, Dodoo, Daniel PLoS ONE, 2013;8(12):e84181. Species: Human Sample Type: Plasma Application: ELISA Development

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Related Product & Information

Background

ICAM-1/CD54

background_content

Background: ICAM-1/CD54 Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

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Citations

1. High plasma levels of soluble intercellular adhesion molecule (ICAM)-1 are associated with cerebral malaria.
   Authors: Adukpo, Selorme, Kusi, Kwadwo A, Ofori, Michael, Tetteh, John K A, Amoako-Sakyi, Daniel, Goka, Bamenla, Adjei, George O, Edoh, Dominic, Akanmori, Bartholo, Gyan, Ben A, Dodoo, Daniel
   PLoS ONE, 2013;8(12):e84181.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


2. Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques.
   Authors: Clauser S, Meilhac O, Bieche I, Raynal P, Bruneval P, Michel JB, Borgel D
   Thromb. Haemost., 2011;107(1):140-9.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


3. Development and validation of sandwich ELISA microarrays with minimal assay interference.
   Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
   J. Proteome Res., 2008;7(6):2406-14.
   Species: Human
   Sample Type: Serum
   Application: ELISA Microarray Development


4. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
   Authors: Pfleger C, Schloot N, ter Veld F
   J. Immunol. Methods, 2007;329(1):214-8.
   Species: Human
   Sample Type: Serum
   Application: Luminex Development


5. Opposing roles of blood myeloid and plasmacytoid dendritic cells in HIV-1 infection of T cells: transmission facilitation versus replication inhibition.
   Authors: Groot F, van Capel TM, Kapsenberg ML, Berkhout B, de Jong EC
   Blood, 2006;108(6):1957-64.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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