抗Shh抗体(Anti-Shh, N-terminal, Rat-Mono antibody)

• 2025/12/01

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  [RSD]厳選 抗体＆リコンビナントタンパク質 30％OFFキャンペーン[～2026/02/27] NEW

  期間：2025/12/01 ～2026/02/27

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  R&D Systems, Inc.（RSD）

• 価格
• Product Details
• Applications and Data
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	E. coli-derived recombinant mouse Sonic Hedgehog N-TerminusCys25-Gly198 (Lys122Arg)Accession # Q62226
Source	Monoclonal Rat IgG2A Clone # 171028
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse Sonic Hedgehog (Shh) N-terminal peptide in ELISAs. In ELISAs, this antibody shows approximately 10% cross-reactivity with recombinant human (rh) Shh N-terminal peptide but no cross-reactivity with rmDhh N-terminal (aa 23‑198) or C‑terminal (aa 199‑396) peptides, rmShh C-terminal peptide (aa 199-437), or rmHIP.

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Applications and Data

	Recommended Concentration	Sample
Mouse Sonic Hedgehog Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse Sonic Hedgehog/Shh N-Terminus Antibody (Catalog #MAB4641 )
ELISA Detection (Matched Antibody Pair)	0.5-2.0 µg/mL	Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody (Catalog #BAM4642 )
ELISA Standard		Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein (Catalog #461-SH )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sonic Hedgehog/Shh	Shh is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened.
Entrez Gene IDs:	6469 (Human); 20423 (Mouse)
Alternate Names:	HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS

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Related Research Areas

Axon Guidance and Neuron Migration

EMT Induction

Hedgehog Family

Hedgehogs

Morphogens

Retinal Stem Cell Markers

Sonic hedgehog and other soluble factors from differentiating embryoid bodies inhibit pancreas development. Authors: Mfopou JK, De Groote V, Xu X, Heimberg H, Bouwens L Stem Cells, 2007;25(5):1156-65. Species: Mouse Sample Type: Whole Cells Application: ELISA Development

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Related Product & Information

Background	Sonic Hedgehog/Shh
background_content	Background: Sonic Hedgehog/Shh Shh is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened.

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Citations

1. Sonic hedgehog and other soluble factors from differentiating embryoid bodies inhibit pancreas development.
   Authors: Mfopou JK, De Groote V, Xu X, Heimberg H, Bouwens L
   Stem Cells, 2007;25(5):1156-65.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ELISA Development

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