抗CXCL5抗体(Anti-CXCL5, Rat, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27760
CXCL5に対する抗体(Anti-CXCL5, Rat, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月20日 08時15分現在]
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Anti-CXCL5, Rat, Goat-Poly, Biotin <Anti-LIX> |
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[在庫・価格 ：2024年05月20日 08時15分現在]
Anti-CXCL5, Rat, Goat-Poly, Biotin <Anti-LIX>
文献数： 1
- 商品コード：BAF543
- メーカー：RSD
- 包装：50μg
- 価格：¥119,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Rat LIX サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB543，スタンダードとして#543-RL-025を用いる。 別名：LIX Genbank No： 6374 Protein Accession No： P97885 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Rat | 免疫動物 | Goat |
| 交差性 | Rat | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 |
M1/M2 Macrophage Activation Marker |
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| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Rat |
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| Label | Biotin |
| Immunogen | E. coli-derived recombinant rat LIXAla38-Arg125Accession # P97885 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects rat LIX in ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse CXCL5/GCP2 is observed, and less than 5% cross-reactivity with recombinant human CXCL6/GCP2 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Rat LIX (Catalog # 543-RL) |
| Rat LIX Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Rat LIX Antibody (Catalog #MAB543 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Rat LIX Biotinylated Antibody (Catalog #BAF543 ) |
| ELISA Standard | Recombinant Rat LIX Protein (Catalog #543-RL ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: LIX | CXCL5, also known as LPS-induced CXC chemokine (LIX), is a chemokine originally cloned from LPS-stimulated mouse fibroblasts. Rat LIX shares approximately 74% amino acid (aa) sequence identity with mouse LIX and is likely the rat orthologue to mouse LIX. Rat LIX cDNA encodes a 130 aa residue precursor with a predicted 37 aa residue signal peptide and a 93 aa residue mature protein. Among human CXC chemokines, rat LIX is most closely related to human CXCL6/GCP-2 and CXCL5/ENA-78. Rat LIX also differs from these two human proteins by having an extended carboxy-terminus. The amino-terminal 115 residues of rat LIX shares 59% and 53% aa sequence homology with human GCP-2 and ENA-78, respectively. It is not clear if LIX should be considered an orthologue of GCP-2 or ENA-78. Yet, mouse LIX was alternatively named mouse GCP-2. Purified recombinant rat LIX is C-terminally truncated. This is consistent with the observation that natural mouse LIX purified from fibroblasts and epithelial cells also contains multiple amino-terminal and carboxy-terminal truncated isoforms. The shorter isoforms of the natural protein were reported to be more active than the longer forms. The bioactivity of rat LIX can be mediated through CXCR2. |
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References
| Proost, P. et al. (1993) J. Immunol. 150:1000. | |
| Smith, J.B. and H.R. Herschman (1995) J. Biol. Chem. 270:16756. | |
| Rovai, L.E. et al. (1997) J. Immunol. 158:5257. | |
| Wuyts, A. et al. (1997) J. Immunol. 157:1736. | |
| Entrez Gene IDs: | 20311 (Mouse); 60665 (Rat) |
| Alternate Names: | LIX |
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Related Research Areas
| Chemokines and Receptors | ||
| CXC Chemokines and Receptors | ||
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Related Product & Information
| Background | LIX |
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| background_content | Background: LIX CXCL5, also known as LPS-induced CXC chemokine (LIX), is a chemokine originally cloned from LPS-stimulated mouse fibroblasts. Rat LIX shares approximately 74% amino acid (aa) sequence identity with mouse LIX and is likely the rat orthologue to mouse LIX. Rat LIX cDNA encodes a 130 aa residue precursor with a predicted 37 aa residue signal peptide and a 93 aa residue mature protein. Among human CXC chemokines, rat LIX is most closely related to human CXCL6/GCP-2 and CXCL5/ENA-78. Rat LIX also differs from these two human proteins by having an extended carboxy-terminus. The amino-terminal 115 residues of rat LIX shares 59% and 53% aa sequence homology with human GCP-2 and ENA-78, respectively. It is not clear if LIX should be considered an orthologue of GCP-2 or ENA-78. Yet, mouse LIX was alternatively named mouse GCP-2. Purified recombinant rat LIX is C-terminally truncated. This is consistent with the observation that natural mouse LIX purified from fibroblasts and epithelial cells also contains multiple amino-terminal and carboxy-terminal truncated isoforms. The shorter isoforms of the natural protein were reported to be more active than the longer forms. The bioactivity of rat LIX can be mediated through CXCR2. |
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Citations
- Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines.
Authors: Miura TA, Wang J, Holmes KV, Mason RJ
Virology, 2007;369(2):288-98.
Species: Rat
Sample Type: Cell Culture Supernates
Application: ELISA Development
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