抗IL-8抗体(Anti-IL-8, Porcine, Goat-Poly, Biotin antibody)

• 2025/12/01

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  [RSD]厳選 抗体＆リコンビナントタンパク質 30％OFFキャンペーン[～2026/02/27] NEW

  期間：2025/12/01 ～2026/02/27

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  R&D Systems, Inc.（RSD）

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Porcine
Label	Biotin
Immunogen	E. coli-derived recombinant porcine IL-8/CXCL8Ala26-Gln104Accession # CAA43461
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects porcine IL-8/CXCL8 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human (rh) IL-8/CXCL8, rhGCP-2, rhIP-10, recombinant mouse (rm) CRG-2, rmI-TAC, rhMIG and rmMIG is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN)
Porcine IL-8/CXCL8 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Porcine IL-8/CXCL8 Antibody (Catalog #MAB5351 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Porcine IL-8/CXCL8 Biotinylated Antibody (Catalog #BAF535 )
ELISA Standard		Recombinant Porcine IL-8/CXCL8 Protein (Catalog #535-IN )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-8/CXCL8	Interleukin 8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of pro‑inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc. IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro-inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. IL-8 has also been reported to be angiogenic both in vivo and in vitro.

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References

Van Damme, J. et al. (1998) in The Cytokine Handbook, A.W. Thomson ed., Academic Press, New York. p. 271.
Long Name:	Interleukin 8
Entrez Gene IDs:	3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names:	3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

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Related Research Areas

Adipocytokines

Alpha Granule Soluble Molecules

Cancer Biomarkers

Chemokines and Receptors

Chemokines and Receptors in Allergy and Asthma

Chemokines and Receptors in Angiogenesis

CXC Chemokines and Receptors

Diabetes

HIF Transcription Factors

Infectious Diseases

Inflammatory Mediators

Macrophage Activation Markers

Megakaryocytes

Molecules Secreted by VSMC

Neuroinflammation

Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets Authors: S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts Infect. Immun., 2017;85(4):. Species: Porcine Sample Type: Serum Application: ELISA detection

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Related Product & Information

Background

IL-8/CXCL8

background_content

Background: IL-8/CXCL8 Interleukin 8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety ofpro‑inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 is a member of the alpha (C-X-C) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc. IL-8 is a potent chemoattractant for neutrophils. In addition, IL-8 also has a wide range of other pro-inflammatory effects. IL-8 causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. IL-8 has also been reported to be angiogenic both in vivo and in vitro.

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Citations

1. Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets
   Authors: S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts
   Infect. Immun., 2017;85(4):.
   Species: Porcine
   Sample Type: Serum
   Application: ELISA detection


2. The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia.
   Authors: Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E
   Intensive Care Med, 2007;33(6):1085-93.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


3. Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology.
   Authors: Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M
   Cytometry A, 2006;69(5):391-5.
   Species: Porcine
   Sample Type: Serum
   Application: Luminex Development


4. Acute hyperinsulinemia restrains endotoxin-induced systemic inflammatory response: an experimental study in a porcine model.
   Authors: Brix-Christensen V, Andersen SK, Andersen R, Mengel A, Dyhr T, Andersen NT, Schmitz O, Orskov H, Tonnesen E
   Anesthesiology, 2004;100(4):861-70.
   Species: Porcine
   Sample Type: Plasma
   Application: ELISA Development


5. Classical swine fever virus induces proinflammatory cytokines and tissue factor expression and inhibits apoptosis and interferon synthesis during the establishment of long-term infection of porcine vascular endothelial cells.
   Authors: Bensaude E, Turner JL, Wakeley PR, Sweetman DA, Pardieu C, Drew TW, Wileman T, Powell PP
   J. Gen. Virol., 2004;85(0):1029-37.
   Species: Porcine
   Sample Type: Cell Culture Supernates
   Application: ELISA Development

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