抗CINC-3抗体(Anti-CINC-3, Rat, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27744
CINC-3に対する抗体(Anti-CINC-3, Rat, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
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[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-CINC-3, Rat, Goat-Poly, Biotin
文献数： 2
- 商品コード：BAF525
- メーカー：RSD
- 包装：50μg
- 価格：¥119,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Rat CXCL2/CINC-3 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB525，スタンダードとして#525-C3-025を用いる。 別名：chemokine (C-X-C motif) ligand 2 Genbank No： 2920 Protein Accession No： P30348 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Rat | 免疫動物 | Goat |
| 交差性 | Rat | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Rat |
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| Label | Biotin |
| Immunogen | E. coli-derived recombinant rat CXCL2/GRO beta /MIP‑2/CINC‑3Ser32-Asn100Accession # P30348 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects rat CXCL2/GRO beta /MIP‑2/CINC‑3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant rat (rr) CINC‑2 beta is observed and less than 0.1% cross-reactivity with recombinant mouse (rm) MIG, rmMIP-2, rrCINC-1, and rrCINC-2 alpha is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Rat CXCL2/GRO beta /MIP‑2/CINC‑3 (Catalog # 525-C3) |
| Rat CXCL2/CINC-3 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Rat CXCL2/GRO beta /MIP‑2/CINC‑3 Antibody (Catalog #MAB525 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Rat CXCL2/GRO beta /MIP‑2/CINC‑3 Biotinylated Antibody (Catalog #BAF525 ) |
| ELISA Standard | Recombinant Rat CXCL2/CINC-3 Protein (Catalog #525-C3 ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CXCL2/GRO beta/MIP-2/CINC-3 | Cytokine-induced neutrophil chemoattractant 3 (CINC-3) has been found to be expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. Based on its protein and DNA sequences, CINC-3 (also known as rat MIP-2, GRO beta and CXCL2) is a member of the alpha (CXC) subfamily of chemokines. CINC-3 cDNA encodes a 100 amino acid (aa) residue precursor protein with a 31 aa signal peptide that is cleaved to yield a 69 aa mature secreted protein. The protein sequence of rat CINC-3 shares approximately 88% identity with murine MIP-2. Characteristic of ELR containing CXC chemokines, CINC-3 is known to be a potent chemotactic factor for rat neutrophils in vitro and in vivo. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3 specific receptors as well as a second common receptor shared by all CINCs. |
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References
| Nakagawa, H. et al. (1994) Biochem. J. 301:545. | |
| Huang, S. et al. (1992) Am. J. Pathol. 141:981. | |
| Entrez Gene IDs: | 2920 (Human); 20310 (Mouse); 114105 (Rat) |
| Alternate Names: | chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2 |
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Related Research Areas
| Chemokines and Receptors in Angiogenesis | ||
| CXC Chemokines and Receptors | ||
| Inflammatory Mediators | ||
| Macrophage Activation Markers | ||
| Molecules Secreted by VSMC | ||
| Neuroinflammation | ||
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Related Product & Information
| Background | CXCL2/GRO beta/MIP-2/CINC-3 |
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| background_content | Background: CXCL2/GRO beta/MIP-2/CINC-3 Cytokine-induced neutrophil chemoattractant 3 (CINC-3) has been found to be expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. Based on its protein and DNA sequences, CINC-3 (also known as rat MIP-2, GRO beta and CXCL2) is a member of the alpha (CXC) subfamily of chemokines. CINC-3 cDNA encodes a 100 amino acid (aa) residue precursor protein with a 31 aa signal peptide that is cleaved to yield a 69 aa mature secreted protein. The protein sequence of rat CINC-3 shares approximately 88% identity with murine MIP-2. Characteristic of ELR containing CXC chemokines, CINC-3 is known to be a potent chemotactic factor for rat neutrophils in vitro and in vivo. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3 specific receptors as well as a second common receptor shared by all CINCs. |
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Citations
- Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines.
Authors: Miura TA, Wang J, Holmes KV, Mason RJ
Virology, 2007;369(2):288-98.
Species: Rat
Sample Type: Cell Culture Supernates
Application: ELISA Development - Cardiac inflammation contributes to right ventricular dysfunction following experimental pulmonary embolism in rats.
Authors: Watts JA, Zagorski J, Gellar MA, Stevinson BG, Kline JA
J. Mol. Cell. Cardiol., 2006;41(2):296-307.
Species: Rat
Sample Type: Tissue Homogenates
Application: ELISA Development
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