抗MIG抗体(Anti-MIG, Mouse, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27716
MIGに対する抗体(Anti-MIG, Mouse, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
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[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-MIG, Mouse, Goat-Poly, Biotin
文献数： 7
- 商品コード：BAF492
- メーカー：RSD
- 包装：50μg
- 価格：¥119,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Mouse CXCL9/MIG サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#AF-492-NA，スタンダードとして#492-MM-010を用いる。 別名：chemokine (C-X-C motif) ligand 9 Genbank No： 4283 Protein Accession No： P18340 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Mouse | 免疫動物 | Goat |
| 交差性 | Mouse | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
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| Label | Biotin |
| Immunogen | E. coli-derived recombinant mouse CXCL9/MIG (R&D Systems, Catalog # 492-MM)Accession # P18340 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse CXCL9/MIG in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross‑reactivity with rhCXCL9 and 0.005% cross‑reactivity with rmMIP‑2, rrCINC‑1, rhGRO alpha, rhGRO beta and rhGRO gamma is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse CXCL9/MIG (Catalog # 492-MM) |
| Mouse CXCL9/MIG Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Mouse CXCL9/MIG Antibody (Catalog #AF-492-NA ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse CXCL9/MIG Biotinylated Antibody (Catalog #BAF492 ) |
| ELISA Standard | Recombinant Mouse CXCL9/MIG Protein (Catalog #492-MM ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CXCL9/MIG | CXCL9, also known as MIG, is a member of the alpha subfamily of chemokines that lacks the ELR domain, and was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse CXCL9 cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The mouse CXCL9 cDNA encodes a 126 amino acid residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield a 105 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes. |
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References
| Loetscher, M. et al. (1996) J. Exp. Med. 184:963. | |
| Liao, F. et al. (1995) J. Exp. Med. 182:1301. | |
| Vanguri, P. (1995) J. Neuroimmunol. 56:35. | |
| Entrez Gene IDs: | 4283 (Human); 17329 (Mouse); 246759 (Rat) |
| Alternate Names: | chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; HuMIG; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma |
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Related Research Areas
| Cancer Biomarkers | ||
| Chemokines and Receptors | ||
| Chemokines and Receptors in Angiogenesis | ||
| CXC Chemokines and Receptors | ||
| Inflammatory Mediators | ||
| Macrophage Activation Markers | ||
| Molecules Secreted by VSMC | ||
| Regulatory T Cells (Tregs) | ||
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Related Product & Information
| Background | CXCL9/MIG |
|---|---|
| background_content | Background: CXCL9/MIG CXCL9, also known as MIG, is a member of the alpha subfamily of chemokines that lacks the ELR domain, and was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse CXCL9 cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The mouse CXCL9 cDNA encodes a 126 amino acid residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield a 105 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes. |
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Citations
- IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
Cell. Physiol. Biochem., 2017;42(5):1961-1972.
Species: Mouse
Sample Type: Serum
Application: ELISA detection - Radiation-inducible immunotherapy for cancer: senescent tumor cells as a cancer vaccine.
Authors: Meng Y, Efimova EV, Hamzeh KW
Mol. Ther., 2012;20(5):1046-55.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded - Antagonist of interferon-inducible protein 10/CXCL10 ameliorates the progression of autoimmune sialadenitis in MRL/lpr mice.
Authors: Hasegawa H, Inoue A, Kohno M, Muraoka M, Miyazaki T, Terada M, Nakayama T, Yoshie O, Nose M, Yasukawa M
Arthritis Rheum., 2006;54(4):1174-83.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded - A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
Authors: Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A
Blood, 2005;107(5):2112-22.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC - Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment.
Authors: Janatpour MJ, Hudak S, Sathe M, McEvoy LM
J. Exp. Med., 2001;194(9):1375-84.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC
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