抗MIG抗体(Anti-MIG, Mouse, Goat-Poly, Biotin antibody)

• 2025/12/01

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  [RSD]厳選 抗体＆リコンビナントタンパク質 30％OFFキャンペーン[～2026/02/27] NEW

  期間：2025/12/01 ～2026/02/27

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  R&D Systems, Inc.（RSD）

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	E. coli-derived recombinant mouse CXCL9/MIG (R&D Systems, Catalog # 492-MM)Accession # P18340
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CXCL9/MIG in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross‑reactivity with rhCXCL9 and 0.005% cross‑reactivity with rmMIP‑2, rrCINC‑1, rhGRO alpha, rhGRO beta and rhGRO gamma is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CXCL9/MIG (Catalog # 492-MM)
Mouse CXCL9/MIG Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	0.2-0.8 µg/mL	Mouse CXCL9/MIG Antibody (Catalog #AF-492-NA )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CXCL9/MIG Biotinylated Antibody (Catalog #BAF492 )
ELISA Standard		Recombinant Mouse CXCL9/MIG Protein (Catalog #492-MM )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL9/MIG	CXCL9, also known as MIG, is a member of the alpha  subfamily of chemokines that lacks the ELR domain, and was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse CXCL9 cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The mouse CXCL9 cDNA encodes a 126 amino acid residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield a 105 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

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References

Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
Liao, F. et al. (1995) J. Exp. Med. 182:1301.
Vanguri, P. (1995) J. Neuroimmunol. 56:35.
Entrez Gene IDs:	4283 (Human); 17329 (Mouse); 246759 (Rat)
Alternate Names:	chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; HuMIG; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma

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Related Research Areas

Cancer Biomarkers

Chemokines and Receptors

Chemokines and Receptors in Angiogenesis

CXC Chemokines and Receptors

Inflammatory Mediators

Macrophage Activation Markers

Molecules Secreted by VSMC

Regulatory T Cells (Tregs)

IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3 Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie Cell. Physiol. Biochem., 2017;42(5):1961-1972. Species: Mouse Sample Type: Serum Application: ELISA detection

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Related Product & Information

Background

CXCL9/MIG

background_content

Background: CXCL9/MIG CXCL9, also known as MIG, is a member of the alpha  subfamily of chemokines that lacks the ELR domain, and was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse CXCL9 cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The mouse CXCL9 cDNA encodes a 126 amino acid residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield a 105 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

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Citations

1. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
   Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
   Cell. Physiol. Biochem., 2017;42(5):1961-1972.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA detection


2. Radiation-inducible immunotherapy for cancer: senescent tumor cells as a cancer vaccine.
   Authors: Meng Y, Efimova EV, Hamzeh KW
   Mol. Ther., 2012;20(5):1046-55.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


3. Antagonist of interferon-inducible protein 10/CXCL10 ameliorates the progression of autoimmune sialadenitis in MRL/lpr mice.
   Authors: Hasegawa H, Inoue A, Kohno M, Muraoka M, Miyazaki T, Terada M, Nakayama T, Yoshie O, Nose M, Yasukawa M
   Arthritis Rheum., 2006;54(4):1174-83.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
   Authors: Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A
   Blood, 2005;107(5):2112-22.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment.
   Authors: Janatpour MJ, Hudak S, Sathe M, McEvoy LM
   J. Exp. Med., 2001;194(9):1375-84.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC

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