抗MCP-1抗体(Anti-MCP-1, Mouse, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	E. coli-derived recombinant mouse CCL2/JE/MCP-1 (R&D Systems, Catalog # 479-JE)Gln24-Arg96Accession # Q5SVU3
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CCL2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.01% cross‑reactivity with rhMCP‑1, rhMCP‑2, rhMCP‑3, rmMARC, rhMCP‑4, rmMCP‑5, rmMDC, rmMIP‑1a, rmMIP‑1b, rmMIP‑1g, rmMIP‑2, rmMIP‑3b, rmRANTES, rmTARC and rmTECK is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CCL2/JE/MCP‑1 (Catalog # 479-JE)
Mouse CCL2/JE/MCP-1 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	0.2-0.8 µg/mL	Mouse CCL2/JE/MCP‑1 Antibody (Catalog #AF-479-NA )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CCL2/JE/MCP‑1 Biotinylated Antibody (Catalog #BAF479 )
ELISA Standard		Recombinant Mouse CCL2/JE/MCP-1 Protein (Catalog #479-JE )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL2/JE/MCP-1	Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

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References

Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
Gu, L. et al. (1999) Chem. Immunol. 72:7.
Luini, W. et al. (1994) Cytokine 6:28.
Entrez Gene IDs:	6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Alternate Names:	C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

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Related Research Areas

Alpha Granule Soluble Molecules

CC Chemokines and Receptors

Chemokines and Receptors

Chemokines and Receptors in Allergy and Asthma

Chemokines and Receptors in Angiogenesis

gamma delta T Cells

HIF Transcription Factors

Inflammatory Mediators

Macrophage Activation Markers

Molecules Secreted by VSMC

Neuroinflammation

Uptake of Amyloid beta by Macrophages

Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development

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Related Product & Information

Background

CCL2/JE/MCP-1

background_content

Background: CCL2/JE/MCP-1 Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

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Citations

1. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


2. Radiation-inducible immunotherapy for cancer: senescent tumor cells as a cancer vaccine.
   Authors: Meng Y, Efimova EV, Hamzeh KW
   Mol. Ther., 2012;20(5):1046-55.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


3. Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells.
   Authors: Puddu P, Latorre D, Carollo M, Catizone A, Ricci G, Valenti P, Gessani S
   PLoS ONE, 2011;6(7):e22504.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


4. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
   Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
   J. Immunol., 2009;183(9):5799-806.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


5. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
   Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
   Immunology, 2008;125(2):272-9.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Development


6. Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
   Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
   J. Immunol., 2006;177(1):612-20.
   Species: Mouse
   Sample Type: BALF
   Application: ELISA Development


7. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
   Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
   J. Immunol., 2006;177(3):1967-74.
   Species: Mouse
   Sample Type: Plasma
   Application: ELISA Development


8. Sequential ELISA to profile multiple cytokines from small volumes.
   Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
   J. Immunol. Methods, 2005;302(1):172-81.
   Species: Mouse
   Sample Type: Plasma
   Application: ELISA Development


9. A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
   Authors: Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A
   Blood, 2005;107(5):2112-22.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


10. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
    Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
    Blood, 2004;104(5):1404-10.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development

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