抗Fractalkine抗体(Anti-Fractalkine, Mouse, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27698
Fractalkineに対する抗体(Anti-Fractalkine, Mouse, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月20日 19時55分現在]
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[在庫・価格 ：2024年05月20日 19時55分現在]
Anti-Fractalkine, Mouse, Goat-Poly, Biotin
文献数： 2
- 商品コード：BAF472
- メーカー：RSD
- 包装：50μg
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Mouse CX3CL1/Fractalkine サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB571-500，スタンダードとして#472-FF-025を用いる。 別名：ABCD-3 Genbank No： 6376 Protein Accession No： O35188 |
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| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Mouse | 免疫動物 | Goat |
| 交差性 | Mouse | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
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| Label | Biotin |
| Immunogen | E. coli-derived recombinant mouse CX3CL1/Fractalkine (R&D Systems, Catalog # 458-MF)Leu22-Lys105Accession # O35188 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse CX3CL1/Fractalkine in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross‑reactivity with recombinant human (rh) Fractalkine (chemokine domain) is observed, less than 0.3% cross‑reactivity with rhFractalkine (full length) is observed and less than 0.1% cross‑reactivity with recombinant rat Fractalkine (full length and chemokine domain) is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse CX3CL1/Fractalkine Full Length (Catalog # 458-MF) |
| Mouse CX3CL1/Fractalkine Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog #MAB571 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (Catalog #BAF472 ) |
| ELISA Standard | Recombinant Mouse CX3CL1/Fractalkine (Full Length) Protein (Catalog #472-FF ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CX3CL1/Fractalkine | CX3CL1, also named neurotactin, is a member of the delta chemokine subfamily that contains a novel C-X3-C motif. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Mouse CX3CL1 cDNA encodes a 395 amino acid (aa) residue precursor protein with a predicted 24 or an alternate 21 aa residue signal peptide, a 76 aa residue globular chemokine domain, a 236 aa residue stalk region rich in Gly, Pro, Ser and Thr and containing degenerate mucin-like repeats, a 21 aa residue transmembrane segment and a 38 aa residue cytoplasmic domain. The extracellular domain of mouse and human CX3CL1 can potentially be released as a soluble protein by proteolysis at the conserved dibasic motif proximal to the transmembrane region. With the exception of the stalk region, human and mouse CX3CL1 share a high degree of amino acid sequence homology (> 75% sequence identity). CX3CL1 is expressed in various tissues including heart, brain, lung, kidney, skeletal muscle, and testis. In rat brain, CX3CL1 expression was found to be localized principally to neurons. The expression of CX3CL1 was also reported to be up-regulated on activated endothelial cells. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes. The gene for mouse CX3CL1 has been mapped to chromosome 11. CX3CR1, previously named V28 or chemokine beta receptor-like 1, has been found to be a specific receptor for CX3CL1. In addition, US28, a 7TM receptor encoded by human cytomegalovirus that binds multiple CC chemokines, has also been shown to bind CX3CL1 with high-affinity. |
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References
| Kledal, T.N. et al. (1998) FEBS Lett. 441:209. | |
| Combadiere, C. et al. (1998) J. Biol. Chem. 273:23799. | |
| Harrison, J.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10896. | |
| Rossi, D.L. et al. (1998) Genomics 47:163. | |
| Entrez Gene IDs: | 6376 (Human); 20312 (Mouse); 89808 (Rat) |
| Alternate Names: | ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1 |
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Related Research Areas
| Cancer Stem Cell Markers | ||
| Chemokines and Receptors | ||
| Chemokines and Receptors in Allergy and Asthma | ||
| Chemokines and Receptors in Angiogenesis | ||
| CX3C Chemokines and Receptors | ||
| Macrophage Activation Markers | ||
| Molecules Secreted by VSMC | ||
| Neuroinflammation | ||
| Renal Markers | ||
| Special Glycoproteins | ||
| Uptake of Amyloid beta by Macrophages | ||
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Related Product & Information
| Background | CX3CL1/Fractalkine |
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| background_content | Background: CX3CL1/Fractalkine CX3CL1, also named neurotactin, is a member of the delta chemokine subfamily that contains a novel C-X3-C motif. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Mouse CX3CL1 cDNA encodes a 395 amino acid (aa) residue precursor protein with a predicted 24 or an alternate 21 aa residue signal peptide, a 76 aa residue globular chemokine domain, a 236 aa residue stalk region rich in Gly, Pro, Ser and Thr and containing degenerate mucin-like repeats, a 21 aa residue transmembrane segment and a 38 aa residue cytoplasmic domain. The extracellular domain of mouse and human CX3CL1 can potentially be released as a soluble protein by proteolysis at the conserved dibasic motif proximal to the transmembrane region. With the exception of the stalk region, human and mouse CX3CL1 share a high degree of amino acid sequence homology (> 75% sequence identity). CX3CL1 is expressed in various tissues including heart, brain, lung, kidney, skeletal muscle, and testis. In rat brain, CX3CL1 expression was found to be localized principally to neurons. The expression of CX3CL1 was also reported to be up-regulated on activated endothelial cells. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes. The gene for mouse CX3CL1 has been mapped to chromosome 11. CX3CR1, previously named V28 or chemokine beta receptor-like 1, has been found to be a specific receptor for CX3CL1. In addition, US28, a 7TM receptor encoded by human cytomegalovirus that binds multiple CC chemokines, has also been shown to bind CX3CL1 with high-affinity. |
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Citations
- Effect of fractalkine-Fc delivery in experimental lung metastasis using DNA/704 nanospheres.
Authors: Richard-Fiardo P, Cambien B, Pradelli E
Cancer Gene Ther., 2011;18(11):761-72.
Species: Mouse
Sample Type: Cell Lysates
Application: WB
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