抗GRO抗体(Anti-GRO, Mouse, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	E. coli-derived recombinant mouse CXCL1/KCArg20-Lys96Accession # P12850
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CXCL1/GRO alpha /KC/CINC-1 in ELISAs and Western blots. In sandwich immunoassays, less than 40% cross-reactivity with recombinant rat (rr) CINC-1 is observed and less than 0.05% cross-reactivity with rrCINC-2 alpha, rrCINC-2 beta, recombinant mouse (rm) CRG-2, rmMIG, and rmMIP-2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20-96 (Catalog # 453-KC)
Mouse CXCL1/KC Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody (Catalog #MAB453 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CXCL1/GRO alpha /KC/CINC‑1 Biotinylated Antibody (Catalog #BAF453 )
ELISA Standard		Recombinant Mouse CXCL1/KC Protein (Catalog #453-KC )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL1/GRO alpha/KC/CINC-1	KC, a member of the alpha (CXC) chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet-derived growth factor. KC cDNA encodes a 96 amino acid (aa) residue precursor protein with a predicted secretory signal peptide that is removed to yield the mature protein. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. In addition to mouse fibroblasts, KC is expressed in macrophages and endothelial cells. Mouse KC is a potent neutrophil attractant and activator. The functional receptor for KC has been identified as CXCR2. Based on the pattern of KC expression in a number of inflammatory disease models, KC appears to have an important role in inflammation. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. Many chemokines are substrates for selective proteolysis at the amino-terminus by various proteases including dipeptidyl peptidase IV or matrix metalloproteases, resulting in truncated chemokine isoforms with different (both enhanced or reduced) bioactivities. The naturally occurring 68 aa N-terminal truncated isoform of mouse KC is reported to be a more potent synergistic growth stimulants for CFU-GM. As a chemoattractant for hCXCR2 transfected mouse Baf/3 cells, the truncated form of mouse KC (aa 29-96) is also approximately 5-fold more active than 77 aa residue form of KC (aa 20-96, R&D Systems, Catalog # 453-KC) (1-4).

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References

King, A.G. et al. (2000) J. Immunol. 164:3774.
Oquendo, P. et al. (1989) J. Biol. Chem. 264:4133.
Huo, Y. et al. (2001) J. Clin. Invest. 108:1307.
Xia, M. et al. (2002) J. Neuroimmunol. 122:55.
Entrez Gene IDs:	2919 (Human); 14825 (Mouse); 81503 (Rat)
Alternate Names:	CINC1; CINC-1; CXCL1; GRO alpha; KC; MGSA-alpha

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Related Research Areas

Show

Alpha Granule Soluble Molecules
Chemokines and Receptors in Allergy and Asthma
Chemokines and Receptors in Angiogenesis
CXC Chemokines and Receptors
Inflammatory Mediators
Macrophage Activation Markers
Nlrp12 Mediates Adverse Neutrophil Recruitment during Influenza Virus Infection Authors: EE Hornick, B Banoth, AM Miller, ZR Zacharias, N Jain, ME Wilson, KN Gibson-Cor, KL Legge, GA Bishop, FS Sutterwala, SL Cassel J. Immunol., 2017;0(0):. Species: Mouse Sample Type: Whole Cells Application: Flow
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Excellent		ELISA	Mouse	Anonymous	01/05/2018			Image Details	ELISA: Mouse CXCL1/GRO alpha /KC/CINC-1 Biotinylated Antibody [BAF453].

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Summary

Application	ELISA
Sample Tested	Serum and Plasma
Species	Mouse
Other Experimental Details	This antibody was used as a matched pair with MAB453 in an ELISA measuring KC in mouse samples.

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Related Product & Information

Background

CXCL1/GRO alpha/KC/CINC-1

background_content

Background: CXCL1/GRO alpha/KC/CINC-1 KC, a member of the alpha (CXC) chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet-derived growth factor. KC cDNA encodes a 96 amino acid (aa) residue precursor protein with a predicted secretory signal peptide that is removed to yield the mature protein. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. In addition to mouse fibroblasts, KC is expressed in macrophages and endothelial cells. Mouse KC is a potent neutrophil attractant and activator. The functional receptor for KC has been identified as CXCR2. Based on the pattern of KC expression in a number of inflammatory disease models, KC appears to have an important role in inflammation. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. Many chemokines are substrates for selective proteolysis at the amino-terminus by various proteases including dipeptidyl peptidase IV or matrix metalloproteases, resulting in truncated chemokine isoforms with different (both enhanced or reduced) bioactivities. The naturally occurring 68 aa N-terminal truncated isoform of mouse KC is reported to be a more potent synergistic growth stimulants for CFU-GM. As a chemoattractant for hCXCR2 transfected mouse Baf/3 cells, the truncated form of mouse KC (aa 29-96) is also approximately 5-fold more active than 77 aa residue form of KC (aa 20-96, R&D Systems, Catalog # 453-KC) (1-4).

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Citations

1. Nlrp12 Mediates Adverse Neutrophil Recruitment during Influenza Virus Infection
   Authors: EE Hornick, B Banoth, AM Miller, ZR Zacharias, N Jain, ME Wilson, KN Gibson-Cor, KL Legge, GA Bishop, FS Sutterwala, SL Cassel
   J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
   Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
   Cell. Physiol. Biochem., 2017;42(5):1961-1972.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA detection


3. Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice
   Authors: Clara Jana-Lui Busch
   Hepatology, 2016;0(0):.
   Species: Mouse
   Sample Type: Peritoneal Lavage Fluid
   Application: ELISA detection


4. MicroRNA-146a provides feedback regulation of lyme arthritis but not carditis during infection with Borrelia burgdorferi.
   Authors: Lochhead R, Ma Y, Zachary J, Baltimore D, Zhao J, Weis J, O'Connell R, Weis J
   PLoS Pathog, 2014;10(6):e1004212.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA Development


5. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
   Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
   J Immunol, 2013;190(10):5178-86.
   Species: Mouse
   Sample Type: BALF
   Application: ELISA detection


6. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


7. Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
   Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
   Am. J. Pathol., 2011;179(6):2730-9.
   Species: Mouse
   Sample Type: BALF
   Application: ELISA Development


8. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
   Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
   Microbes Infect., 2009;11(4):524-30.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


9. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
   Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
   Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5954-9.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Development


10. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008;125(2):272-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development

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