抗MIP-1α抗体(Anti-MIP-1α, Mouse, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	E. coli-derived recombinant mouse CCL3/MIP-1 alpha (R&D Systems, Catalog # 450-MA)Ala24-Ala92Accession # Q5QNW0
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse MIP-1 alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with rhCCL3, rmCCL9/10 and rmCCL4 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CCL3/MIP‑1 alpha (Catalog # 450-MA)
Mouse CCL3/MIP-1 alpha Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	0.2-0.8 µg/mL	Mouse CCL3/MIP‑1 alpha Antibody (Catalog #AF-450-NA )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CCL3/MIP‑1 alpha Biotinylated Antibody (Catalog #BAF450 )
ELISA Standard		Recombinant Mouse CCL3/MIP-1 alpha Protein (Catalog #450-MA )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL3/MIP-1 alpha	The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP-1 alpha shares approximately 77% and 70% amino acid identity with human MIP-1 alpha and mouse MIP-1 beta, respectively. MIP-1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP-1 proteins are members of the beta (C-C) subfamily of chemokines. Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha  has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, MIP-1 beta was reported to be much less active. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.

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References

Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
Entrez Gene IDs:	6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine)
Alternate Names:	C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; Mip1a; MIP-1alpha; MIP1-alpha; Scya3

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Related Research Areas

Show

Alpha Granule Soluble Molecules
CC Chemokines and Receptors
Chemokines and Receptors
Chemokines and Receptors in Allergy and Asthma
Chemokines and Receptors in Angiogenesis
Group 1 ILCs
Infectious Diseases
Inflammatory Mediators
Macrophage Activation Markers
Molecules Secreted by VSMC
Multiple Myeloma
Neuroinflammation
Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Very Good		ELISA	Mouse	Anonymous	11/27/2017			Image Details	ELISA: Mouse CCL3/MIP-1 alpha Biotinylated Antibody [BAF450].

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Summary

Application	ELISA
Sample Tested	Serum and Plasma
Species	Mouse
Other Experimental Details	Together with antibody AF450 this antibody was used to create an ELISA to measure MIP-1 alpha in mouse samples.

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Related Product & Information

Background

CCL3/MIP-1 alpha

background_content

Background: CCL3/MIP-1 alpha The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP-1 alpha shares approximately 77% and 70% amino acid identity with human MIP-1 alpha and mouse MIP-1 beta, respectively. MIP-1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP-1 proteins are members of the beta (C-C) subfamily of chemokines. Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha  has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, MIP-1 beta was reported to be much less active. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.

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Citations

1. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


2. Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
   Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
   Am. J. Pathol., 2011;179(6):2730-9.
   Species: Mouse
   Sample Type: BALF
   Application: ELISA Development


3. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
   Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
   J. Immunol., 2009;183(9):5799-806.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


4. Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS).
   Authors: Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
   J. Biol. Chem., 2009;0(0):.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


5. Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
   Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
   Eur. J. Immunol., 2006;36(8):2025-34.
   Species: Mouse
   Sample Type: Tissue Secretion
   Application: ELISA Development


6. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
   Authors: Yang L, Mosmann T
   Eur. J. Immunol., 2004;34(6):1617-26.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


7. Selective macrophage suppression during sepsis.
   Authors: Ellaban E, Bolgos G, Remick D
   Cell. Immunol., 2004;231(1):103-11.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


8. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
   Authors: Mosmann T
   Eur. J. Immunol., 2004;34(6):1617-26.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


9. A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
   Authors: Reading PC, Symons JA, Smith GL
   J. Immunol., 2003;170(3):1435-42.
   Species: Human
   Sample Type: BALF
   Application: ELISA Development


10. Immunostimulatory DNA-based vaccines elicit multifaceted immune responses against HIV at systemic and mucosal sites.
    Authors: Horner AA, Datta SK, Takabayashi K, Belyakov IM, Cinman N, Nguyen MD, Van Uden JH, Berzofsky JA
    J. Immunol., 2001;167(3):1584-91.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ELISpot Development

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