抗IL-17抗体(Anti-IL-17, Mouse, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Summary
• Summary
• Summary
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	E. coli-derived recombinant mouse IL‑17 (R&D Systems, Catalog # 421-ML)Thr22-Ala158Accession # Q62386
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IL-17 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human IL‑17 is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL‑17 (Catalog # 421-ML)
Mouse IL-17 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse IL‑17/IL‑17A Antibody (Catalog #MAB721 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse IL‑17 Biotinylated Antibody (Catalog #BAF421 )
ELISA Standard		Recombinant Mouse IL-17A Protein (Catalog #421-ML )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17/IL-17A	Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mouse IL-17 shows 57% and 87% sequence identity with herpes virus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8- T cells, the expression of mouse IL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.

目次に戻る

References

Kennedy, J. et al. (1996) J. Interferon Cytokine Res. 16:611.
Yao, Z. et al. (1995) J. Immunol. 155:5483.
Yao, Z. et al. (1995) Immunity 3:811.
Rouvier, E. et al. (1993) J. Immunol. 150:5445.
Long Name:	Interleukin 17
Entrez Gene IDs:	3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
Alternate Names:	CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

目次に戻る

Related Research Areas

Show

B Cell Chemotaxis, Migration, and Adhesion
Cytokines and Receptors in Tfh Cell Differentiation and Function
gamma delta T Cells
Helper T (Th) Cells
IL-17 Family
LTi Cells
Macrophage Activation Markers
Molecules Secreted by VSMC
Multiple Sclerosis and Myelin Related Molecules
Natural Killer T (NKT) Cells
NCR-ILC3
Neuroinflammation
T Cell Cytokine Signaling
Th17 Cells
The Major Histocompatibility Complex Class III Haplotype Ltab-Ncr3 Regulates Adjuvant-Induced but Not Antigen-Induced Autoimmunity Authors: AC Yau, J Tuncel, R Holmdahl Am. J. Pathol, 2017;0(0):. Species: Rat Sample Type: Tissue Homogenates Application: ELISA detection
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Excellent		ELISA	Mouse	Anonymous	11/27/2017			Image Details	ELISA: Mouse IL-17 Biotinylated Antibody [BAF421].

目次に戻る

Summary

Show

Application	ELISA
Sample Tested	Serum and Plasma
Species	Mouse
Other Experimental Details	This antibody was used as the detection in an ELISA with MAB421 as the capture.
	Excellent		Flow	oct	Anonymous	08/11/2017

目次に戻る

Summary

Show

Application	Flow Cytometry
Sample Tested	293T human embryonic kidney cell line
Species	oct
	Excellent		ELISA	Mouse	Anonymous	02/22/2016

目次に戻る

Summary

Application	ELISA
Sample Tested	Bone marrow-derived dendritic cells,Mouse spleenocytes,Liver tissue
Species	Mouse

目次に戻る

Related Product & Information

Background

IL-17/IL-17A

background_content

Background: IL-17/IL-17A Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mouse IL-17 shows 57% and 87% sequence identity with herpes virus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8- T cells, the expression of mouse IL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.

目次に戻る

Citations

1. The Major Histocompatibility Complex Class III Haplotype Ltab-Ncr3 Regulates Adjuvant-Induced but Not Antigen-Induced Autoimmunity
   Authors: AC Yau, J Tuncel, R Holmdahl
   Am. J. Pathol, 2017;0(0):.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: ELISA detection


2. Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis
   Authors: SH Lee, JY Kwon, SY Kim, K Jung, ML Cho
   Sci Rep, 2017;7(1):10133.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


3. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
   Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
   Cell. Physiol. Biochem., 2017;42(5):1961-1972.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA detection


4. Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis
   Authors: Jung-Yeon Lim
   Sci Rep, 2016;6(0):26851.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


5. MHC class II alleles associated with Th1 rather than Th17 type immunity drive the onset of early arthritis in a rat model of rheumatoid arthritis
   Eur. J. Immunol, 2016;0(0):.
   Species: Rat
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


6. Interferon gamma suppresses collagen-induced arthritis by regulation of Th17 through the induction of indoleamine-2,3-deoxygenase.
   Authors: Lee J, Lee J, Park M, Lim M, Park E, Kim E, Yang E, Lee S, Jhun J, Park S, Kim H, Cho M
   PLoS ONE, 2013;8(4):e60900.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


7. CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction.
   Authors: Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C
   Eur. J. Immunol., 2012;42(5):1250-60.
   Species: Mouse
   Sample Type: Pleural Lavage Fluid
   Application: ELISA Development


8. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


9. Hepatic interleukin-7 expression regulates T cell responses.
   Authors: Sawa Y, Arima Y, Ogura H, Kitabayashi C, Jiang JJ, Fukushima T, Kamimura D, Hirano T, Murakami M
   Immunity, 2009;30(3):447-57.
   Species: Mouse
   Sample Type: Serum
   Application: WB


10. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
    Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5954-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development

目次に戻る