抗IGF-Ⅰ Receptor抗体(Anti-IGF-Ⅰ Receptor, Human, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	recombinant human IGF-I R extracellular domainAccession # P08069
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IGF-I R in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) IGF‑I, rhIGF‑II, rhIGFBP-1, rhIGFBP-2, rhIGFBP-3, rhIGFBP-4, rhIGFBP-5, and rhIGFBP-6 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IGF-I R (Catalog # 391-GR)
Human IGF-I R Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human/Mouse IGF‑I R Antibody (Catalog #MAB391 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human IGF‑I R Biotinylated Antibody (Catalog #BAF391 )
ELISA Standard		Recombinant Human IGF-I R Protein, CF (Catalog #391-GR )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGF-I R	IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

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References

Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.
Long Name:	Insulin-like Growth Factor I Receptor
Entrez Gene IDs:	3480 (Human)
Alternate Names:	CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2

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Related Research Areas

Adipocytokines

Cancer Biomarkers

Endocrine Regulation of Lipid Metabolism

Glucose Homeostasis

IGF Family

Metabolic Peptide Hormones and Regulators

Peptide Receptors on VSMC

Platelet Cytokine and Growth Factor Receptors

Receptor Tyrosine Kinases (RTKs)

Receptor Tyrosine Kinases (RTKs) in the Akt Pathway

Receptors in the Jak/STAT Pathway

Estrogen receptor, progesterone receptor, interleukin-6 and interleukin-8 are variable in breast cancer and benign stem/progenitor cell populations. Authors: Schillace R, Skinner A, Pommier R, O'Neill S, Muller P, Naik A, Hansen J, Pommier S BMC Cancer, 2014;14(0):733. Species: Human Sample Type: Cell Lysates Application: Functional Assay

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Related Product & Information

Background

IGF-I R

background_content

Background: IGF-I R IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

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Citations

1. Estrogen receptor, progesterone receptor, interleukin-6 and interleukin-8 are variable in breast cancer and benign stem/progenitor cell populations.
   Authors: Schillace R, Skinner A, Pommier R, O'Neill S, Muller P, Naik A, Hansen J, Pommier S
   BMC Cancer, 2014;14(0):733.
   Species: Human
   Sample Type: Cell Lysates
   Application: Functional Assay


2. Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes.
   Authors: Back K, Arnqvist HJ
   Growth Horm. IGF Res., 2009;19(2):101-11.
   Species: Human
   Sample Type: Cell Lysates
   Application: ELISA Development

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