抗HCC-1抗体(Anti-HCC-1, Human, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27577
HCC-1に対する抗体(Anti-HCC-1, Human, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月18日 00時00分現在]
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Anti-HCC-1, Human, Goat-Poly, Biotin |
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[在庫・価格 ：2024年05月18日 00時00分現在]
Anti-HCC-1, Human, Goat-Poly, Biotin
文献数： 1
- 商品コード：BAF324
- メーカー：RSD
- 包装：50μg
- 価格：¥111,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Human CCL14a/HCC-1 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB3241，スタンダードとして#324-HC-010を用いる。 別名：CCL14a，HCC-1 Genbank No： 6358 Protein Accession No： Q16627 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Goat |
| 交差性 | Human | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Biotin |
| Immunogen | E. coli-derived recombinant human CCL14a/HCC-1 (R&D Systems, Catalog # 324-HC)Thr20-Asn93Accession # Q16627 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human CCL14a/HCC-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) CCL16, rhCCL8, rhCCL7, rhCCL4, and rhCCL3 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human CCL14a/HCC‑1 aa 20-93 (Catalog # 324-HC) |
| Human CCL14a/HCC-1 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human CCL14/HCC‑1/HCC‑3 Antibody (Catalog #MAB3241 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human CCL14a/HCC‑1 Biotinylated Antibody (Catalog #BAF324 ) |
| ELISA Standard | Recombinant Human CCL14a/HCC-1 Protein (Catalog #324-HC ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CCL14a/HCC-1 | HCC-1 (Hemofiltrate CC Chemokine-1) was originally isolated from the hemofiltrate of human patients with chronic renal failure (1). It belongs to the CC chemokine superfamily and has been designated CCL14a. HCC-1/CCL14a cDNA encodes a 93 amino acid (aa) residue precursor with a 19 aa signal peptide that is cleaved to form the 74 aa secreted protein (aa 20 - 93). By alternative splicing, a second longer isoform named HCC-3/CCL14b, which includes sequences from exon 3, also exists (2). HCC-1/CCL14a is expressed constitutively in various normal tissues including spleen, liver, muscle, gut and bone marrow. It circulates at nanomolar concentrations in human plasma. Different post-translationally modified HCC-1/CCL14a, including O-glycosylated and N-terminally truncated variants of HCC-1/CCL14a, have been identified (3, 4). Whereas the 74 aa peptide is a weak CCR1 agonist, the proteolytically processed, truncated HCC-1/CCL14a (aa 28 - 93) is a highly potent agonist of CCR1, CCR5 and to a lesser extent, CCR3. HCC-1/CCL14a (aa 28 - 93) promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1. Activation of the HCC-1/CCL14a precursor to active peptide is mediated by the urokinase type plasminogen activator or plasmin (5). |
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References
| Schulz-Knappe, P. et al. (1996) J. Exp. Med. 183:295. | |
| Forssmanns, U. et al. (2001) J. Leukocyte Biology 70:357. | |
| Richter, R. et al. (2000) Biochemistry 39:10799. | |
| Munch, J. et al. (2002) Antimicrob. Agents Chemother. 46:982. | |
| Vakili, J. et al. (2001) J. Immunol. 167:3406. | |
| Entrez Gene IDs: | 6358 (Human) |
| Alternate Names: | CCL14; CCL14a; HCC1; HCC-1 |
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Related Research Areas
| CC Chemokines and Receptors | ||
| Chemokines and Receptors | ||
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Related Product & Information
| Background | CCL14a/HCC-1 |
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| background_content | Background: CCL14a/HCC-1 HCC-1 (Hemofiltrate CC Chemokine-1) was originally isolated from the hemofiltrate of human patients with chronic renal failure (1). It belongs to the CC chemokine superfamily and has been designated CCL14a. HCC-1/CCL14a cDNA encodes a 93 amino acid (aa) residue precursor with a 19 aa signal peptide that is cleaved to form the 74 aa secreted protein (aa 20 - 93). By alternative splicing, a second longer isoform named HCC-3/CCL14b, which includes sequences from exon 3, also exists (2). HCC-1/CCL14a is expressed constitutively in various normal tissues including spleen, liver, muscle, gut and bone marrow. It circulates at nanomolar concentrations in human plasma. Different post-translationally modified HCC-1/CCL14a, including O-glycosylated and N-terminally truncated variants of HCC-1/CCL14a, have been identified (3, 4). Whereas the 74 aa peptide is a weak CCR1 agonist, the proteolytically processed, truncated HCC-1/CCL14a (aa 28 - 93) is a highly potent agonist of CCR1, CCR5 and to a lesser extent, CCR3. HCC-1/CCL14a (aa 28 - 93) promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1. Activation of the HCC-1/CCL14a precursor to active peptide is mediated by the urokinase type plasminogen activator or plasmin (5). |
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Citations
- Recognition versus adaptive up-regulation and degradation of CC chemokines by the chemokine decoy receptor D6 are determined by their N-terminal sequence.
Authors: Savino B, Borroni EM, Torres NM, Proost P, Struyf S, Mortier A, Mantovani A, Locati M, Bonecchi R
J. Biol. Chem., 2009;284(38):26207-15.
Species: Human
Sample Type: Cell Lysates
Application: WB
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