抗Flt-1抗体(Anti-Flt-1, Human, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human VEGF R1/Flt-1Ser27-His687Accession # NP_001153392
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human VEGF R1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse VEGF R1, recombinant human (rh) VEGF R2, and rhVEGF R3 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human VEGF R1/Flt‑1 Fc Chimera (Catalog # 321-FL)
Immunohistochemistry	5-15 µg/mL	See below

Immunohistochemistry

VEGF R1/Flt‑1 in Human Breast. VEGF R1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human breast array using Human VEGF R1/Flt‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF321) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Long Name	Vascular Endothelial Growth Factor Receptor 1
Background	VEGF R1/Flt-1
background_content	Background: VEGF R1/Flt-1 VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (Flt-1, KDR/Flk-1, Flt-4, tie-1, and tek/tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. Flt-1, KDR/Flk-1 and Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis. The Flt-1 gene has been localized to chromosome 13q12. Full length Flt-1 mRNA encodes a 1338 amino acid (aa) residue precursor with a 22 aa residue signal peptide. Mature Flt-1 is composed of a 736 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 558 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of Flt-1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned.

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Citations

1. Characteristics of the specific humoral response in patients with advanced solid tumors after active immunotherapy with a VEGF vaccine, at different antigen doses and using two distinct adjuvants
   Authors: J Sánchez Ra, Y Morera Día, M Bequet-Rom, F Hernández-, KH Selman-Hou, A de la Torr, ER Santiesteb, Y Martín Bau, CH Bermúdez B, J de la Torr, JV Gavilondo,, M Ayala Avil
   BMC Immunol., 2017;18(1):39.
   Species: Human
   Sample Type: Serum
   Application: ELISA detection


2. Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease
   Sci Rep, 2016;6(0):32382.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC Not Specified


3. PEDF derived from glial Muller cells: a possible regulator of retinal angiogenesis.
   Authors: Eichler W, Yafai Y, Keller T, Wiedemann P, Reichenbach A
   Exp. Cell Res., 2004;299(1):68-78.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
   Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
   Eur. J. Clin. Invest., 2003;33(10):883-90.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development

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