抗Eotaxin抗体(Anti-Eotaxin, Human, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27574
Eotaxinに対する抗体(Anti-Eotaxin, Human, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
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Anti-Eotaxin, Human, Goat-Poly, Biotin |
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[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-Eotaxin, Human, Goat-Poly, Biotin
文献数： 6
- 商品コード：BAF320
- メーカー：RSD
- 包装：50μg
- 価格：¥119,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human CCL11/Eotaxin サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB320-500，スタンダードとして#320-EO-020を用いる。 別名：C-C motif chemokine 11 Genbank No： 6356 Protein Accession No： Q6I9T4 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Goat |
| 交差性 | Human | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Biotin |
| Immunogen | E. coli-derived recombinant human CCL11/Eotaxin (R&D Systems, Catalog # 320-EO)Gly24-Pro97Accession # Q6I9T4 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human CCL11/Eotaxin in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant mouse (rm) CCL11, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rhMIP-1 alpha, and rhRANTES is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) |
| Human CCL11/Eotaxin Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human CCL11/Eotaxin Antibody (Catalog #MAB320 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human CCL11/Eotaxin Biotinylated Antibody (Catalog #BAF320 ) |
| ELISA Standard | Recombinant Human CCL11/Eotaxin Protein (Catalog #320-EO ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CCL11/Eotaxin | CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2 and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1 - 3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4). |
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References
| Kitamura, M. et al. (1996) J. Biol. Chem 271:7725. | |
| Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449. | |
| Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604. | |
| Choe, H. et al. (1996) Cell 85:1135. | |
| Entrez Gene IDs: | 6356 (Human); 20292 (Mouse) |
| Alternate Names: | C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin) |
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Related Research Areas
| Adult Neurogenesis | ||||||||||
| Airway Inflammation and Remodeling | ||||||||||
| CC Chemokines and Receptors | ||||||||||
| Chemokines and Receptors | ||||||||||
| Chemokines and Receptors in Allergy and Asthma | ||||||||||
| Chemokines and Receptors in Angiogenesis | ||||||||||
| Eosinophils | ||||||||||
| Macrophage Activation Markers | ||||||||||
| Molecules Secreted by VSMC | ||||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|---|
 Enlarge | Excellent | ELISA | Human | Anonymous | 11/16/2017 | Image Details |
 ELISA: Human CCL11/Eotaxin Biotinylated Antibody [BAF320]. | |||
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Summary
| Application | ELISA |
| Sample Tested | Serum and Plasma |
| Species | Human |
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Related Product & Information
| Background | CCL11/Eotaxin |
|---|---|
| background_content | Background: CCL11/Eotaxin CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2 and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1 - 3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4). |
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Citations
- Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
Authors: Osei-Kumah A, Smith R, Clifton VL
Cytokine, 2008;43(2):187-93.
Species: Human
Sample Type: Plasma
Application: ELISA Development - Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clin. Immunol., 2006;120(2):220-31.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development - Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission.
Authors: Hajnicka V, Vancova I, Kocakova P, Slovak M, Gasperik J, Slavikova M, Hails RS, Labuda M, Nuttall PA
Parasitology, 2005;130(0):333-42.
Species: Human
Sample Type: Saliva
Application: ELISA Development - Chemoattractant factors in breast milk from allergic and nonallergic mothers.
Authors: Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP
Pediatr. Res., 2000;47(5):592-7.
Species: Human
Sample Type: Serum
Application: ELISA Development
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