抗HGF抗体(Anti-HGF, Human, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27561
HGFに対する抗体(Anti-HGF, Human, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年10月25日 13時35分現在]
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Anti-HGF, Human, Goat-Poly, Biotin |
|
10 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2025年10月25日 13時35分現在]
Anti-HGF, Human, Goat-Poly, Biotin
文献数： 10
- 商品コード：BAF294
- メーカー：RSD
- 包装：100μg
- 価格：¥93,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human HGF サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB694-500，スタンダードとして#294-HG-005を用いる。 別名：deafness Genbank No： 3082 Protein Accession No： P14210 |
||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Goat |
| 交差性 | Human | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
|
||
| 製品記事 | |||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
||
追加しました。
Product Details
| Species Reactivity | Human |
|---|---|
| Label | Biotin |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGF (R&D Systems, Catalog # 294-HG)Accession # P14210 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human HGF in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human (rh) HGF R, recombinant mouse HGF R, and rhMSP is observed. |
追加しました。
Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human HGF (Catalog # 294-HG) |
| Human HGF Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human HGF Antibody (Catalog #MAB694 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human HGF Biotinylated Antibody (Catalog #BAF294 ) |
| ELISA Standard | Recombinant Human HGF Protein (Catalog #294-HG ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: HGF | HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15). |
|
追加しました。
References
| Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40. | |
| Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21. | |
| Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3. | |
| Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501. | |
| Nakamura, T. et al. (1989) Nature 342:440. | |
| Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131. | |
| Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039. | |
| Corso, S. et al. (2005) Trends Mol. Med. 11:284. | |
| Maeshima, A. et al. (2000) Kid. Int. 58:1511. | |
| Montesano, R. et al. (1991) Cell 67:901. | |
| Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261. | |
| Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531. | |
| Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472. | |
| Mineo, R. et al. (1994) Endocrinology 145:4355. | |
| Urbanek, K. et al. (2005) Circ. Res. 97:663. | |
| Long Name: | Hepatocyte Growth Factor |
| Entrez Gene IDs: | 3082 (Human); 15234 (Mouse); 403441 (Canine) |
| Alternate Names: | deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A |
追加しました。
Related Research Areas
| Adhesion Molecules in Angiogenesis | ||||||||||
| Alpha Granule Soluble Molecules | ||||||||||
| Cytokines and Receptors in Angiogenesis | ||||||||||
| EMT Induction | ||||||||||
| Gametes and Fertilization | ||||||||||
| Hepatic Endoderm | ||||||||||
| Integrins and Associated Molecules | ||||||||||
| Pancreatic Endoderm | ||||||||||
| Preeclampsia | ||||||||||
| Semaphorins, Plexin Receptors, and Related Molecules | ||||||||||
| ||||||||||
| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|---|
 Enlarge | Excellent | ELISA | Human | Anonymous | 11/16/2017 | Image Details |
 ELISA: Human HGF Biotinylated Antibody [BAF294]. | |||
追加しました。
Summary
| Application | ELISA |
| Sample Tested | Serum and Plasma |
| Species | Human |
| Other Experimental Details | A sandwich ELISA was made with MAB694 as the capture and BAF294 as the detection using 294-HG as the immunoassay calibrator. |
追加しました。
Related Product & Information
| Background | HGF |
|---|---|
| background_content | Background: HGF HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15). |
追加しました。
Citations
- Novel antibody reagents for characterization of drug- and tumor microenvironment-induced changes in epithelial-mesenchymal transition and cancer stem cells
Authors: T Navas, TD Pfister, S Colantonio, A Aziz, L Dieckman, RG Saul, J Kaczmarczy, S Borgel, SY Alcoser, MG Hollingshe, YH Lee, DP Bottaro, T Hiltke, G Whiteley, N Takebe, RJ Kinders, RE Parchment, JE Tomaszewsk, JH Doroshow
PLoS ONE, 2018;13(6):e0199361.
Species: Mouse
Sample Type: Plasma
Application: ELISA detection - A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010;56(0):1320.
Species: Human
Sample Type: Cell Culture Supernates
Application: Luminex Development - A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation.
Authors: Tatsumi R, Wuollet AL, Tabata K, Nishimura S, Tabata S, Mizunoya W, Ikeuchi Y, Allen RE
Am. J. Physiol., Cell Physiol., 2009;296(4):C922-9.
Species: Rat
Sample Type: Cell Culture Supernates
Application: ELISA Development - Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008;7(6):2406-14.
Species: Human
Sample Type: Serum
Application: ELISA Microarray Development
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。