抗MCP-2抗体(Anti-MCP-2, Human, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Research Areas
• Summary
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	E. coli-derived recombinant human CCL8/MCP-2 (R&D Systems, Catalog # 281-CP)Gln24-Pro99Accession # P80075
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CCL8/MCP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) HCC‑1, recombinant mouse (rm) MIP-1 alpha, rhMCP-1, and rhMCP-3 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP)
Human CCL8/MCP-2 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human CCL8/MCP‑2 Antibody (Catalog #MAB281 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human CCL8/MCP‑2 Biotinylated Antibody (Catalog #BAF281 )
ELISA Standard		Recombinant Human CCL8/MCP-2 Protein (Catalog #281-CP )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL8/MCP-2	MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2. Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
Entrez Gene IDs:	6355 (Human); 20307 (Mouse)
Alternate Names:	C-C motif chemokine 8; CCL8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; member 8 (monocyte chemotactic protein 2); monocyte chemoattractant protein 2; SCYA10; small-inducible cytokine A8

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Related Research Areas

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CC Chemokines and Receptors
Chemokines and Receptors
Chemokines and Receptors in Allergy and Asthma
Infectious Diseases
Inflammatory Mediators
Macrophage Activation Markers
Molecules Secreted by VSMC
A Toll-like receptor-1 variant and its characteristic cellular phenotype is associated with severe malaria in Papua New Guinean children. Authors: Manning L, Cutts J, Stanisic D, Laman M, Carmagnac A, Allen S, O'Donnell A, Karunajeewa H, Rosanas-Urgell A, Siba P, Davis T, Michon P, Schofield L, Rockett K, Kwiatkowski D, Mueller I Genes Immun, 2016;17(1):52-9. Species: Human Sample Type: Cell Culture Supernates Application: ELISA detection
Images	Ratings	Applications	Species	Reviewed By	Date	Details
	Excellent		ELISA	Human	Anonymous	12/07/2017

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Summary

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Application	ELISA
Sample Tested	Serum
Species	Human
Other Experimental Details	Good detection in disease samples
Enlarge	Very Good		ELISA	Human	Anonymous	11/20/2017			Image Details	ELISA: Human CCL8/MCP-2 Biotinylated Antibody [BAF281].

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Summary

Application	ELISA
Sample Tested	Serum and Plasma
Species	Human
Other Experimental Details	This antibody was used with MAB281 to build an ELISA to measure MCP-2 in human serum samples.

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Related Product & Information

Background

CCL8/MCP-2

background_content

Background: CCL8/MCP-2 MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2. Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

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Citations

1. A Toll-like receptor-1 variant and its characteristic cellular phenotype is associated with severe malaria in Papua New Guinean children.
   Authors: Manning L, Cutts J, Stanisic D, Laman M, Carmagnac A, Allen S, O'Donnell A, Karunajeewa H, Rosanas-Urgell A, Siba P, Davis T, Michon P, Schofield L, Rockett K, Kwiatkowski D, Mueller I
   Genes Immun, 2016;17(1):52-9.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


2. Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
   Authors: Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J
   J Allergy Clin Immunol, 2014;133(4):1227-30.
   Species: Human
   Sample Type: Whole Cells
   Application: ELISA detection


3. Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects.
   Authors: Struyf S, Proost P, Vandercappellen J
   Eur. J. Immunol., 2009;39(3):843-57.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


4. Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production.
   Authors: Tay SS, McCormack A, Rose ML
   Transplantation, 2004;78(7):987-94.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development

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