抗MCP-1抗体(Anti-MCP-1, Human, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27548
MCP-1に対する抗体(Anti-MCP-1, Human, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
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Anti-MCP-1, Human, Goat-Poly, Biotin <Anti-MCAF> |
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[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-MCP-1, Human, Goat-Poly, Biotin <Anti-MCAF>
文献数： 24
- 商品コード：BAF279
- メーカー：RSD
- 包装：50μg
- 価格：¥120,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human CCL2/JE/MCP-1 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB679-500，スタンダードとして#279-MC-010を用いる。 別名：C-C motif chemokine ligand 2 Genbank No： 6347 Protein Accession No： P13500 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Goat |
| 交差性 | Human | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Biotin |
| Immunogen | E. coli-derived recombinant human CCL2/JE/MCP‑1 (R&D Systems, Catalog # 279-MC)Gln24-Thr99Accession # P13500 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human CCL2/JE/MCP‑1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) MCP‑2, rhMCP-3, rhMCP-4, recombinant mouse (rm) CCL2/JE/MCP‑1, and rmMARC is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC) |
| Human CCL2/JE/MCP-1 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human CCL2/JE/MCP‑1 Antibody (Catalog #MAB679 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human CCL2/JE/MCP‑1 Biotinylated Antibody (Catalog #BAF279 ) |
| ELISA Standard | Recombinant Human CCL2/MCP-1 Protein (Catalog #279-MC ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CCL2/JE/MCP-1 | CCL2, also known as monocyte chemotactic and activating factor (MCAF), was initially purified independently by two groups based on its ability to chemoattract monocytes. Subsequent to its cloning and sequencing, it became evident that this protein is also identical to the product of the human JE gene. The JE gene, originally identified in mouse fibroblasts, is a platelet-derived growth factor (PDGF)-inducible gene. The human CCL2 cDNA encodes a 99 amino acid residue precursor protein with a 23 residue hydrophobic signal peptide that is cleaved to generate the 76 residue mature protein. Natural CCL2 is heterogeneous in size due to the addition of O-linked carbohydrates and sialic acid residues. In addition to fibroblasts; tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can also produce CCL2 either constitutively or upon stimulation by various stimuli. CCL2 is a member of the beta (C-C) subfamily of chemokines. The existence of MCP-2 and MCP-3 with 62% and 73% amino acid identity respectively, to CCL2 have been reported. Consistent with it being a member of the chemokine beta family, CCL2 has been shown to chemoattract monocytes. In addition, it will also activate monocytes to be cytostatic for some human tumor cell lines; to increase cytosolic free calcium; to generate and release monocyte superoxide anions and to release monocyte lysosomal enzymes in vitro. CCL2 was reported to be capable of regulating adhesion molecule expression and cytokine production in human monocytes as well as chemoattracting, activating, and inducing histamine release from basophils. The biological roles played by CCL2 in a number of inflammatory and non-inflammatory disease states characterized by the accumulation of leukocytes at the site of the lesion, including atherosclerosis, delayed hypersensitivity reactions, etc., are being determined. CCL2 can bind to the C-C chemokine receptor-1 that also binds MIP-1 alpha, RANTES and MIP-1 beta. A specific receptor for CCL2 has also been cloned from THP-1 and MonoMac 6 cells. |
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| Entrez Gene IDs: | 6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine) | |
| Alternate Names: | C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF | |
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Related Research Areas
| Alpha Granule Soluble Molecules | ||||||||||
| CC Chemokines and Receptors | ||||||||||
| Chemokines and Receptors | ||||||||||
| Chemokines and Receptors in Allergy and Asthma | ||||||||||
| Chemokines and Receptors in Angiogenesis | ||||||||||
| gamma delta T Cells | ||||||||||
| HIF Transcription Factors | ||||||||||
| Inflammatory Mediators | ||||||||||
| Macrophage Activation Markers | ||||||||||
| Molecules Secreted by VSMC | ||||||||||
| Neuroinflammation | ||||||||||
| Uptake of Amyloid beta by Macrophages | ||||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|---|
 Enlarge | Very Good | ELISA | Human | Anonymous | 11/20/2017 | Image Details |
 ELISA: Human CCL2/JE/MCP‑1 Biotinylated Antibody [BAF279]. | |||
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Summary
| Application | ELISA |
| Sample Tested | Serum and Plasma |
| Species | Human |
| Other Experimental Details | This antibody paired well with MAB279 to build an ELISA to measure MCP-1 in human serum samples. |
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Related Product & Information
| Background | CCL2/JE/MCP-1 |
|---|---|
| background_content | Background: CCL2/JE/MCP-1 CCL2, also known as monocyte chemotactic and activating factor (MCAF), was initially purified independently by two groups based on its ability to chemoattract monocytes. Subsequent to its cloning and sequencing, it became evident that this protein is also identical to the product of the human JE gene. The JE gene, originally identified in mouse fibroblasts, is a platelet-derived growth factor (PDGF)-inducible gene. The human CCL2 cDNA encodes a 99 amino acid residue precursor protein with a 23 residue hydrophobic signal peptide that is cleaved to generate the 76 residue mature protein. Natural CCL2 is heterogeneous in size due to the addition of O-linked carbohydrates and sialic acid residues. In addition to fibroblasts; tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can also produce CCL2 either constitutively or upon stimulation by various stimuli. CCL2 is a member of the beta (C-C) subfamily of chemokines. The existence of MCP-2 and MCP-3 with 62% and 73% amino acid identity respectively, to CCL2 have been reported. Consistent with it being a member of the chemokine beta family, CCL2 has been shown to chemoattract monocytes. In addition, it will also activate monocytes to be cytostatic for some human tumor cell lines; to increase cytosolic free calcium; to generate and release monocyte superoxide anions and to release monocyte lysosomal enzymes in vitro. CCL2 was reported to be capable of regulating adhesion molecule expression and cytokine production in human monocytes as well as chemoattracting, activating, and inducing histamine release from basophils. The biological roles played by CCL2 in a number of inflammatory and non-inflammatory disease states characterized by the accumulation of leukocytes at the site of the lesion, including atherosclerosis, delayed hypersensitivity reactions, etc., are being determined. CCL2 can bind to the C-C chemokine receptor-1 that also binds MIP-1 alpha, RANTES and MIP-1 beta. A specific receptor for CCL2 has also been cloned from THP-1 and MonoMac 6 cells. |
追加しました。
Citations
- Hyperglycemia-induced Renal P2X7 Receptor Activation Enhances Diabetes-related Injury
Authors: RI Menzies, JWR Booth, JJ Mullins, MA Bailey, FWK Tam, JT Norman, RJ Unwin
EBioMedicine, 2017;0(0):.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA detection - Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus
Sci Rep, 2016;6(0):29909.
Species: Human
Sample Type: Serum - Microparticles engineered to highly express peroxisome proliferator-activated receptor-gamma decreased inflammatory mediator production and increased adhesion of recipient monocytes.
Authors: Sahler J, Woeller C, Phipps R
PLoS ONE, 2014;9(11):e113189.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA detection - A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010;56(0):1320.
Species: Human
Sample Type: Cell Culture Supernates
Application: Luminex Development - Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009;87(12):1814-20.
Species: Human
Sample Type: Urine
Application: Antibody Array Development - Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects.
Authors: Struyf S, Proost P, Vandercappellen J
Eur. J. Immunol., 2009;39(3):843-57.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development - Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
J. Immunol., 2009;183(9):5799-806.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development - Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
Clin. Chem., 2007;53(11):2010-2.
Species: Human
Sample Type: Plasma
Application: ELISA Development - Ultrasensitive flow-based immunoassays using single-molecule counting.
Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clin. Chem., 2007;53(11):1990-5.
Species: Human
Sample Type: Plasma
Application: ELISA Development - Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Authors: Pfleger C, Schloot N, ter Veld F
J. Immunol. Methods, 2007;329(1):214-8.
Species: Human
Sample Type: Serum
Application: Luminex Development
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