抗RANTES抗体(Anti-RANTES, Human, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Primate
Label	Biotin
Immunogen	E. coli-derived recombinant human CCL5/RANTESSer24-Ser91Accession # P13501
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human and primate CCL5/RANTES in ELISAs and Western blots.  In sandwich immunoassays, approximately 12% cross‑reactivity with rmCCL5 is observed and less than 0.05% cross‑reactivity with rhIL-8, rhGRO alpha, rhMCP-1, rhMIP-1 alpha and rhMIP‑1 beta is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human CCL5/RANTES (Catalog # 278-RN)
Human/Primate CCL5/RANTES Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human/Primate CCL5/RANTES Antibody (Catalog #MAB678 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human/Primate CCL5/RANTES Biotinylated Antibody (Catalog #BAF278 )
ELISA Standard		Recombinant Human CCL5/RANTES Protein (Catalog #278-RN )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL5/RANTES	CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1 - 3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature human CCL5 shares 75% - 84% aa seqeuence identity with canine, cotton rat, feline, mouse, and rat CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11 - 13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14 - 16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).

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References

Schall, T.J. et al. (1990) Nature 347:669.
Bacon, K.B. et al. (1995) Science 269:1727.
Fischer, F.R. et al. (2001) J. Immunol. 167:1637.
Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477.
Schall, T.J. et al. (1988) J. Immunol. 141:1018.
Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83.
Levy, J.A. (2009) J. Immunol. 182:3945.
Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37.
DeVico, A.L. and Gallo, R.C. (2004) Nat. Rev. Microbiol. 2:401.
Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
Appay, V. et al. (1999) J. Biol. Chem. 274:27505.
Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. 100:1885.
von Hundelshausen, P. et al. (2005) Blood 105:924.
von Hundelshausen, P. et al. (2001) Circulation 103:1772.
Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897.
Baltus, T. et al. (2003) Blood 102:1985.
Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.
Entrez Gene IDs:	6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names:	CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES

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Related Research Areas

Show

Airway Inflammation and Remodeling
Alpha Granule Soluble Molecules
CC Chemokines and Receptors
Chemokines and Receptors
Chemokines and Receptors in Allergy and Asthma
Chemokines and Receptors in Angiogenesis
Eosinophils
Infectious Diseases
Macrophage Activation Markers
Molecules Secreted by VSMC
Neuroinflammation
Oligomerized, filamentous surface presentation of RANTES/CCL5 on vascular endothelial cells. Authors: Oynebraten I, Barois N, Bergeland T, Kuchler A, Bakke O, Haraldsen G Sci Rep, 2015;5(0):9261. Species: Human Sample Type: Whole Cells Application: ICC
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Very Good		ELISA	Human	Anonymous	11/22/2017			Image Details	ELISA: Human/Primate CCL5/RANTES Biotinylated Antibody [BAF278].

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Summary

Application	ELISA
Sample Tested	Serum and Plasma
Species	Human
Other Experimental Details	The RANTES ELISA we made used MAG678 as the capture and BAF278 as the detection. Dilutional linearity of samples wasn't great but otherwise the assay worked very well.

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Related Product & Information

Background

CCL5/RANTES

background_content

Background: CCL5/RANTES CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1 - 3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature human CCL5 shares 75% - 84% aa seqeuence identity with canine, cotton rat, feline, mouse, and rat CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11 - 13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14 - 16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).

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Citations

1. Oligomerized, filamentous surface presentation of RANTES/CCL5 on vascular endothelial cells.
   Authors: Oynebraten I, Barois N, Bergeland T, Kuchler A, Bakke O, Haraldsen G
   Sci Rep, 2015;5(0):9261.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Disrupting functional interactions between platelet chemokines inhibits atherosclerosis in hyperlipidemic mice.
   Authors: Koenen RR, von Hundelshausen P, Nesmelova IV, Zernecke A, Liehn EA, Sarabi A, Kramp BK, Piccinini AM, Paludan SR, Kowalska MA, Kungl AJ, Hackeng TM, Mayo KH, Weber C
   Nat. Med., 2009;15(1):97-103.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
   Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
   AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


4. Relation of circulating concentrations of chemokine receptor CCR5 ligands to C-peptide, proinsulin and HbA1c and disease progression in type 1 diabetes.
   Authors: Pfleger C, Kaas A, Hansen L, Alizadeh B, Hougaard P, Holl R, Kolb H, Roep BO, Mortensen HB, Schloot NC
   Clin. Immunol., 2008;128(1):57-65.
   Species: Human
   Sample Type: Serum
   Application: ELISA Development


5. Development and validation of sandwich ELISA microarrays with minimal assay interference.
   Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
   J. Proteome Res., 2008;7(6):2406-14.
   Species: Human
   Sample Type: Serum
   Application: ELISA Microarray Development


6. Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
   Authors: Osei-Kumah A, Smith R, Clifton VL
   Cytokine, 2008;43(2):187-93.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


7. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
   Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
   AIDS, 2007;21(10):1263-72.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


8. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
   Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
   Clin. Chem., 2007;53(11):2010-2.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


9. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
   Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
   Blood, 2007;109(10):4272-9.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


10. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007;53(11):1990-5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development

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