抗I-309抗体(Anti-I-309, Human, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27542
I-309に対する抗体(Anti-I-309, Human, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年05月01日 00時00分現在]
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[在庫・価格 ：2025年05月01日 00時00分現在]
Anti-I-309, Human, Goat-Poly, Biotin
文献数： 2
- 商品コード：BAF272
- メーカー：RSD
- 包装：50μg
- 価格：¥118,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human CCL1/I-309 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB272-500，スタンダードとして#272-I-010を用いる。 別名：C-C motif chemokine 1 Genbank No： 6346 Protein Accession No： P22362 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Goat |
| 交差性 | Human | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Biotin |
| Immunogen | E. coli-derived recombinant human CCL1/I‑309/TCA‑3 (R&D Systems, Catalog # 272-I)Lys24-Lys96Accession # P22362 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human CCL1/I‑309/TCA‑3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with rhMIP-1 beta, rvMIP-1, rrIFN-gamma, rmTCA-3 and rhTARC is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human CCL1/I‑309/TCA‑3 (Catalog # 272-I) |
| Human CCL1/I-309 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human CCL1/I‑309/TCA‑3 Antibody (Catalog #MAB272 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human CCL1/I‑309/TCA‑3 Biotinylated Antibody (Catalog #BAF272 ) |
| ELISA Standard | Recombinant Human CCL1/I-309 Protein (Catalog #272-I ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CCL1/I-309/TCA-3 | Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes. CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. The CCL1 sequence contains one potential N-linked glycosylation site and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15-16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily. |
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References
| Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci. USA 89:2950. | |
| Miller, M.D. et al. (1990) J. Immunol. 145:2737. | |
| Entrez Gene IDs: | 6346 (Human); 20290 (Mouse) |
| Alternate Names: | C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3 |
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Related Research Areas
| CC Chemokines and Receptors | ||||||||||
| Chemokines and Receptors | ||||||||||
| Chemokines and Receptors in Allergy and Asthma | ||||||||||
| Chemokines and Receptors in Angiogenesis | ||||||||||
| Macrophage Activation Markers | ||||||||||
| Regulatory T Cells (Tregs) | ||||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|---|
 Enlarge | Very Good | ELISA | Human | Anonymous | 11/22/2017 | Image Details |
 ELISA: Human CCL1/I‑309/TCA‑3 Biotinylated Antibody [BAF272]. | |||
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Summary
| Application | ELISA |
| Sample Tested | Serum and Plasma |
| Species | Human |
| Other Experimental Details | MAB272 was used as the capture and BAF272 as the detection in a sandwich immunoassay. The immunoassay standard was 272-I. It worked very well. Most healthy human samples were not detectable. |
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Related Product & Information
| Background | CCL1/I-309/TCA-3 |
|---|---|
| background_content | Background: CCL1/I-309/TCA-3 Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes. CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. The CCL1 sequence contains one potential N-linked glycosylation site and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15-16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily. |
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Citations
- CC chemokine ligand 1 is released into the airways of atopic asthmatics.
Authors: Montes-Vizuet R, Vega-Miranda A, Valencia-Maqueda E, Negrete-Garcia MC, Velasquez JR, Teran LM
Eur. Respir. J., 2006;28(1):59-67.
Species: Human
Sample Type: BALF
Application: ELISA Development - A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
J. Leukoc. Biol., 2001;70(4):617-23.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development
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