抗MIP-1β抗体(Anti-MIP-1β, Human, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) MIP‑1 alpha is observed and less than 0.1% cross-reactivity with recombinant viral (rv) MIP-I, rvMIP-II, rhMIP‑1δ, recombinant mouse (rm) MIP‑1 alpha, rmMIP‑1 beta, rmMIP-1 gamma, rhGRO beta, rhGRO gamma, rhMCP-1, and rhRANTES is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human CCL4/MIP‑1 beta (Catalog # 271-BME)
Immunocytochemistry	5-15 µg/mL	See below
Human CCL4/MIP-1 beta Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human CCL4/MIP‑1 beta Antibody (Catalog #MAB271 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human CCL4/MIP‑1 beta Biotinylated Antibody (Catalog #BAF271 )
ELISA Standard		Recombinant Human CCL4/MIP-1 beta Protein (Catalog #271-BME )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Immunocytochemistry	click image to view larger	CCL4/MIP‑1 beta in Human PBMCs.CCL4/MIP‑1 beta was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human CCL4/MIP‑1 beta Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF271) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL4/MIP-1 beta	CCL4, also known as macrophage inflammatory protein 1 beta (MIP-1 beta ) is a 7.8 kDa beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV. Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2b. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor. Both forms of CCL4 block HIV-1 infection of T cells by inducing the downregulation of CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.
Entrez Gene IDs:	6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine)
Alternate Names:	ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2

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Related Research Areas

CC Chemokines and Receptors

Chemokines and Receptors

Chemokines and Receptors in Allergy and Asthma

Chemokines and Receptors in Angiogenesis

Infectious Diseases

Inflammatory Mediators

Macrophage Activation Markers

Molecules Secreted by VSMC

Neuroinflammation

Regulatory T Cells (Tregs)

Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ Transplantation, 2009;87(12):1814-20. Species: Human Sample Type: Urine Application: Antibody Array Development

Immunocytochemistry

CCL4/MIP‑1 beta in Human PBMCs. CCL4/MIP‑1 beta was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human CCL4/MIP‑1 beta Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF271) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Background

CCL4/MIP-1 beta

background_content

Background: CCL4/MIP-1 beta CCL4, also known as macrophage inflammatory protein 1 beta (MIP-1 beta ) is a 7.8 kDa beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV. Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2b. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor. Both forms of CCL4 block HIV-1 infection of T cells by inducing the downregulation of CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.

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Citations

1. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
   Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
   Transplantation, 2009;87(12):1814-20.
   Species: Human
   Sample Type: Urine
   Application: Antibody Array Development


2. Relation of circulating concentrations of chemokine receptor CCR5 ligands to C-peptide, proinsulin and HbA1c and disease progression in type 1 diabetes.
   Authors: Pfleger C, Kaas A, Hansen L, Alizadeh B, Hougaard P, Holl R, Kolb H, Roep BO, Mortensen HB, Schloot NC
   Clin. Immunol., 2008;128(1):57-65.
   Species: Human
   Sample Type: Serum
   Application: ELISA Development


3. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
   Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
   AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


4. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
   Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
   AIDS, 2007;21(10):1263-72.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


5. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
   Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
   Blood, 2007;109(10):4272-9.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


6. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
   Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
   Clin. Immunol., 2006;120(2):220-31.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


7. Changes in soluble factor-mediated CD8+ cell-derived antiviral activity in cynomolgus macaques infected with simian immunodeficiency virus SIVmac251: relationship to biological markers of progression.
   Authors: Dioszeghy V, Benlhassan-Chahour K, Delache B, Dereuddre-Bosquet N, Aubenque C, Gras G, Le Grand R, Vaslin B
   J. Virol., 2006;80(1):236-45.
   Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


8. Fractalkine reduces N-methyl-d-aspartate-induced calcium flux and apoptosis in human neurons through extracellular signal-regulated kinase activation.
   Authors: Deiva K, Geeraerts T, Salim H, Leclerc P, Hery C, Hugel B, Freyssinet JM, Tardieu M
   Eur. J. Neurosci., 2004;20(12):3222-32.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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