抗CXCL10抗体(Anti-CXCL10, Human, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	E. coli-derived recombinant human CXCL10/IP-10
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CXCL10/IP-10/CRG-2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant mouse CRG‑2, recombinant human (rh) Lymphotactin, rhGRO alpha, and rhMIG is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human CXCL10/IP‑10/CRG‑2 (Catalog # 266-IP)
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	Human peripheral blood monocytes treated with Recombinant Human IFN‑ gamma (Catalog # 285‑IF), fixed with paraformaldehyde, and permeabilized with saponin
Human CXCL10/IP-10 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human CXCL10/IP‑10/CRG‑2 Antibody (Catalog #MAB266 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human CXCL10/IP‑10/CRG‑2 Biotinylated Antibody (Catalog #BAF266 )
ELISA Standard		Recombinant Human CXCL10/IP-10 Protein (Catalog #266-IP )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL10/IP-10/CRG-2	CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts, and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF-alpha, IL-12, and viruses. Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid (aa) sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 aa residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for CXCL10 and MIG has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

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References

Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
Wang, X. et al. (1996) J. Biol. Chem. 271:24286.
Entrez Gene IDs:	3627 (Human); 15945 (Mouse)
Alternate Names:	C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

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Related Research Areas

Show

Chemokines and Receptors
Chemokines and Receptors in Allergy and Asthma
Chemokines and Receptors in Angiogenesis
CXC Chemokines and Receptors
Infectious Diseases
Macrophage Activation Markers
Molecules Secreted by VSMC
Neuroinflammation
Regulatory T Cells (Tregs)
Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus Sci Rep, 2016;6(0):29909. Species: Human Sample Type: Serum
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Very Good		ELISA	Human	Anonymous	11/17/2017			Image Details	ELISA: Human CXCL10/IP‑10/CRG‑2 Biotinylated Antibody [BAF266].

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Summary

Application	ELISA
Sample Tested	Serum and Plasma
Species	Human
Other Experimental Details	Sandwich ELISA using MAB266 on solid phase and BAF266 as detection.

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Related Product & Information

Background

CXCL10/IP-10/CRG-2

background_content

Background: CXCL10/IP-10/CRG-2 CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts, and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF-alpha, IL-12, and viruses. Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid (aa) sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 aa residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for CXCL10 and MIG has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

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Citations

1. Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus
   Sci Rep, 2016;6(0):29909.
   Species: Human
   Sample Type: Serum
   


2. Human rhinovirus-induced ISG15 selectively modulates epithelial antiviral immunity.
   Authors: Zaheer R, Wiehler S, Hudy M, Traves S, Pelikan J, Leigh R, Proud D
   Mucosal Immunol, 2014;7(5):1127-38.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


3. Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
   Authors: Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J
   J Allergy Clin Immunol, 2014;133(4):1227-30.
   Species: Human
   Sample Type: Whole Cells
   Application: ELISA detection


4. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
   Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
   AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


5. Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha.
   Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA
   J. Immunol., 2007;179(7):4840-8.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


6. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
   Authors: Pfleger C, Schloot N, ter Veld F
   J. Immunol. Methods, 2007;329(1):214-8.
   Species: Human
   Sample Type: Serum
   Application: Luminex Development


7. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
   Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
   Clin. Chem., 2007;53(11):2010-2.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


8. Eotaxin-2 and colorectal cancer: a potential target for immune therapy.
   Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE
   Clin. Cancer Res., 2007;13(19):5719-28.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: ELISA Development


9. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
   Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
   AIDS, 2007;21(10):1263-72.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


10. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development

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