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抗TNF Receptor Ⅰ抗体(Anti-TNF Receptor Ⅰ, Human, Goat-Poly, Biotin antibody)

掲載日情報：2021/01/28 現在Webページ番号：27500

アールアンドディー システムズ /
R&D Systems, Inc.
[メーカー略称：RSD]

TNF Receptor Ⅰに対する抗体(Anti-TNF Receptor Ⅰ, Human, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。

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Anti-TNF Receptor I, Human, Goat-Poly, Biotin <Anti-TNFRI>
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 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 7
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説明文
マッチドペア：Human TNF RI/TNFRSF1A サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB625-500，スタンダードとして#636-R1-025を用いる。
別名：CD120a
Genbank No： 7132
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat クラス IgG 標識 Biotin
交差性 Human 適用 ELISA,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
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関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

[在庫・価格 ：2026年04月05日 00時00分現在]

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Anti-TNF Receptor I, Human, Goat-Poly, Biotin <Anti-TNFRI>

文献数： 7

説明文 マッチドペア：Human TNF RI/TNFRSF1A サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB625-500，スタンダードとして#636-R1-025を用いる。
別名：CD120a
Genbank No： 7132
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat
交差性 Human 適用 ELISA,Western Blot
標識 Biotin 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド
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Product Details

Species ReactivityHuman
LabelBiotin
ImmunogenE. coli-derived recombinant human sTNF RI
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects human TNF RI in ELISAs and Western blots. In sandwich immunoassays, less than 0.02% cross-reactivity with recombinant human (rh) TNF‑ alpha, recombinant mouse (rm) TNF‑ alpha, recombinant porcine TNF‑ alpha, recombinant rat TNF‑ alpha, rhTNF‑ beta, rmsTNF RI, rhsTNF RII, and rmsTNF RII is observed.


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Applications and Data

 Recommended
Concentration		Sample
Western Blot0.1 µg/mLRecombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1)
Human TNF RI/TNFRSF1A Sandwich ImmunoassayReagent
ELISA Capture (Matched Antibody Pair)2-8 µg/mL Human TNF RI/TNFRSF1A Antibody (Catalog #MAB625 )
ELISA Detection (Matched Antibody Pair)0.1-0.4 µg/mL Human TNF RI/TNFRSF1A Biotinylated Antibody (Catalog #BAF225 )
ELISA Standard  Recombinant Human sTNF RI/TNFRSF1A Protein (Catalog #636-R1 )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.Preparation and Storage
ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.Reconstitution Buffer Available
ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RI/TNFRSF1A

TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a type I transmembrane protein member of the TNF receptor superfamily member, designated TNFRSF1A (1, 2). Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-  alpha trimer-binding cysteine-rich domains (CRD) in their extracellular domains (ECD). However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1 - 3). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (4). TNF RI is present on the cell surface as a trimer of 55 kDa subunits (4, 5). TNF-alpha induces sequestering of TNF RI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (9, 10). Release of the 28 - 34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 6 - 8). Full-length TNF RI may also be released in exosome-like vesicles (11). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (6, 12, 13). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Human TNF RI is a 455 amino acid (aa) protein that contains a 21 aa signal sequence, a 190 aa ECD with a PLAD domain(5) that mediates constitutive trimer formation, followed by the four CRD, a 23 aa transmembrane domain, and a 221 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (15). The ECD of human TNF RI shows 80%, 80%, 73%, 69% and 70% aa identity with dog, cat, pig, rat and mouse TNF RI, respectively; it shows 23% aa identity with the ECD of TNF RII.

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References

Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
Peschon, J.J. et al. (1998) J. Immunol. 160:943.
Banner, D.W et al. (1993) Cell 73: 431.
Chan, F.K. et al. (2000) Science 288:2351.
Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
Jin, L. et al. (2000) J. Immunol. 165:5153.
Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
Legler, D.F. et al. (2003) Immunity 18:655.
Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
McDermott, M.F. et al. (1999) Cell 97:133.
Schneider-Brachert, W. et al. (2004) Immunity 21:415.
Schall, T.J. et al. (1990) Cell 61:361.
Long Name:Tumor Necrosis Factor Receptor I
Entrez Gene IDs:7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names:CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

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Related Research Areas

Show
Adipocytokines
Cell Structure and Signaling Molecules
Cytokine and Growth Factor Receptors on VSMC
Cytokines and Receptors in Allergy and Asthma
Cytokines and Receptors in Angiogenesis
Diabetes
Diabetic Peripheral Neuropathy
EMT Induction
Endothelial Cell Markers
Neuroinflammation
Obesity
Platelet Cytokine and Growth Factor Receptors
Regulation of Apoptosis by TNF Superfamily Members
T Cell Cytokine Signaling
TNF Superfamily Receptors
  1. Aminopeptidase P3, a new member of the TNF-TNFR2 signaling complex, induces phosphorylation of JNK1 and JNK2.
    Authors: Inoue M, Kamada H, Abe Y, Higashisaka K, Nagano K, Mukai Y, Yoshioka Y, Tsutsumi Y, Tsunoda S
    J Cell Sci, 2015;128(4):656-69.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
ImagesRatingsApplicationsSpeciesReviewed ByDateDetails
ELISA Human TNF RI/TNFRSF1A Biotinylated Antibody BAF225 Array BAF225
Enlarge		Excellent
 ELISA Human Anonymous 11/22/2017 Image Details ELISA Human TNF RI/TNFRSF1A Biotinylated Antibody BAF225
ELISA: Human TNF RI/TNFRSF1A Biotinylated Antibody [BAF225].

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Summary

Application ELISA
Sample Tested Serum and Plasma,Cell culture supernatant
Species Human
Other Experimental Details We used MAB625 as the solid phase antibody, 636-R1 as the immunoassay standard and BAF225 as the detection antibody in a sandwich ELISA. Everything worked very well.


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Related Product & Information

BackgroundTNF RI/TNFRSF1A
background_contentBackground:
TNF RI/TNFRSF1A
TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a type I transmembrane protein member of the TNF receptor superfamily member, designated TNFRSF1A (1, 2). Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-  alpha trimer-binding cysteine-rich domains (CRD) in their extracellular domains (ECD). However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1 - 3). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (4). TNF RI is present on the cell surface as a trimer of 55 kDa subunits (4, 5). TNF-alpha induces sequestering of TNF RI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (9, 10). Release of the 28 - 34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 6 - 8). Full-length TNF RI may also be released in exosome-like vesicles (11). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (6, 12, 13). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Human TNF RI is a 455 amino acid (aa) protein that contains a 21 aa signal sequence, a 190 aa ECD with a PLAD domain(5) that mediates constitutive trimer formation, followed by the four CRD, a 23 aa transmembrane domain, and a 221 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (15). The ECD of human TNF RI shows 80%, 80%, 73%, 69% and 70% aa identity with dog, cat, pig, rat and mouse TNF RI, respectively; it shows 23% aa identity with the ECD of TNF RII.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Aminopeptidase P3, a new member of the TNF-TNFR2 signaling complex, induces phosphorylation of JNK1 and JNK2.
    Authors: Inoue M, Kamada H, Abe Y, Higashisaka K, Nagano K, Mukai Y, Yoshioka Y, Tsutsumi Y, Tsunoda S
    J Cell Sci, 2015;128(4):656-69.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

  2. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Type: Urine
    Application: Antibody Array Development

  3. Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
    Authors: Emsley HC, Smith CJ, Gavin CM
    BMC Neurol, 2007;7(0):5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development

  4. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004;284(1):99-106.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Array Development

  5. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004;80(6):1521-5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development

  6. A pilot study of pentoxifylline for the treatment of primary sclerosing cholangitis.
    Authors: Bharucha AE, Jorgensen R, Lichtman SN, LaRusso NF, Lindor KD
    Am. J. Gastroenterol., 2000;95(9):2338-42.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development

  7. Innate immunity associated with inflammatory responses and cytokine production against common dietary proteins in patients with autism spectrum disorder.
    Authors: Jyonouchi H, Sun S, Itokazu N
    Neuropsychobiology, 0;46(2):76-84.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development



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