抗IL-8抗体(Anti-IL-8, Human, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	E. coli-derived recombinant human IL-8/CXCL8Ser28-Ser99Accession # P10145
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL-8/CXCL8 in ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinantporcine IL-8/CXCL8, recombinant rat (rr) CINC-2 alpha, and rrCINC-2 beta is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL-8/CXCL8 (Catalog # 208-IL)
Immunocytochemistry	5-15 µg/mL	See below
Human IL-8/CXCL8 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human IL-8/CXCL8 Antibody (Catalog #MAB208 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human IL-8/CXCL8 Biotinylated Antibody (Catalog #BAF208 )
ELISA Standard		Recombinant Human IL-8/CXCL8 Protein (Catalog #208-IL )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Immunocytochemistry	click image to view larger	IL-8/CXCL8 in Human PBMCs.IL-8/CXCL8 was detected in immersion fixed PMA-, ionomycin- and monensin-activated human peripheral blood mononuclear cells (PBMCs) using Human IL-8/CXCL8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF208) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-8/CXCL8	CXCL8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc. CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. CXCL8 was also reported to be angiogenic both in vivo and in vitro.
Long Name:	Interleukin 8
Entrez Gene IDs:	3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names:	3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

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Related Research Areas

Adipocytokines

Alpha Granule Soluble Molecules

Cancer Biomarkers

Chemokines and Receptors

Chemokines and Receptors in Allergy and Asthma

Chemokines and Receptors in Angiogenesis

CXC Chemokines and Receptors

Diabetes

HIF Transcription Factors

Infectious Diseases

Inflammatory Mediators

Macrophage Activation Markers

Megakaryocytes

Molecules Secreted by VSMC

Neuroinflammation

Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease Authors: J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard PLoS ONE, 2017;12(9):e0184039. Species: Human Sample Type: Cell Culture Supernates Application: ELISA detection

Immunocytochemistry

IL-8/CXCL8 in Human PBMCs. IL-8/CXCL8 was detected in immersion fixed PMA-, ionomycin- and monensin-activated human peripheral blood mononuclear cells (PBMCs) using Human IL-8/CXCL8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF208) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Background

IL-8/CXCL8

background_content

Background: IL-8/CXCL8 CXCL8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety ofpro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc. CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. CXCL8 was also reported to be angiogenic both in vivo and in vitro.

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Citations

1. Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease
   Authors: J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard
   PLoS ONE, 2017;12(9):e0184039.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


2. Neutrophil Membrane Cholesterol Content is a Key Factor in Cystic Fibrosis Lung Disease
   Authors: MM White, P Geraghty, E Hayes, S Cox, W Leitch, B Alfawaz, GM Lavelle, OJ McElvaney, R Flannery, J Keenan, P Meleady, M Henry, M Clynes, C Gunaratnam, NG McElvaney, EP Reeves
   EBioMedicine, 2017;23(0):173-184.
   Species: Human
   Sample Type: Plasma
   Application: ELISA detection


3. Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition.
   Authors: Voltolini C, Battersby S, Novembri R, Torricelli M, Severi F, Petraglia F, Norman J
   Endocrinology, 2015;156(2):670-9.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


4. Daily exposure to dust alters innate immunity.
   Authors: Sahlander K, Larsson K, Palmberg L
   PLoS ONE, 2012;7(2):e31646.
   Species: Human
   Sample Type: Serum
   Application: ELISA Development


5. Protection from RNA and DNA viruses by IL-32.
   Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
   J. Immunol., 2011;186(7):4110-8.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Electrochemiluminescence


6. Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
   Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
   J. Immunol., 2010;185(11):7047-56.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


7. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
   Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
   Transplantation, 2009;87(12):1814-20.
   Species: Human
   Sample Type: Urine
   Application: Antibody Array Development


8. Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
   Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
   Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(2):L388-400.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


9. Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
   Authors: Osei-Kumah A, Smith R, Clifton VL
   Cytokine, 2008;43(2):187-93.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


10. Endogenous IL-32 controls cytokine and HIV-1 production.
    Authors: Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
    J. Immunol., 2008;181(1):557-65.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Electrochemiluminescence

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