抗TIMP-1抗体(Anti-TIMP-1, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse TIMP-1Cys25-Arg205Accession # P12032
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant rat TIMP-1 is observed, less than 5% cross-reactivity with recombinant human (rh) TIMP-1 is observed, and less than 1% cross-reactivity with rhTIMP-2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse TIMP‑1 Western Blot Standard (Catalog # WBC022)
Immunohistochemistry	5-15 µg/mL	See below
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Mouse TIMP‑1 (Catalog # 980‑MT), see our available Western blot detection antibodies
Neutralization	Measured by its ability to neutralize Recombinant Mouse TIMP-1 (0.07 µg/mL, Catalog # 980-MT) inhibition of Recombinant Mouse/Rat MMP‑2 (0.2 µg/mL, Catalog # 924-MP) cleavage of the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (10 µM, Catalog # ES001). The Neutralization Dose (ND50) is typically 1.3 µg/mL.

Immunohistochemistry
	TIMP-1 in Mouse Ovary. TIMP-1 was detected in perfusion fixed frozen sections of mouse ovary using Goat Anti-Mouse TIMP-1 Antigen Affinity-purified Polyclonal Antibody (red; Catalog # AF980) at 15 µg/mL overnight at 4 °C.

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Related Product & Information

Background

TIMP-1

background_content

Background: TIMP-1 Tissue inhibitors of metalloproteinases or TIMPs are a family of homologous proteins that regulate the activity of matrix metalloproteinases (MMPs) (1, 2). There are four known members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4 that have been found to exhibit multiple functions, including inhibition of active MMPs, pro‑MMP activation, cell growth promotion, matrix binding, inhibition of angiogenesis and the induction of apoptosis. Structurally, TIMPs have two domains, an N‑terminal domain and a C-terminal domain. Each domain consists of three disulfide-bonded loops. TIMP-1 is a glycoprotein produced by a wide range of cell types. Through its N-terminal domain, TIMP-1 inhibits active MMPs by forming a non-covalent binary complex with the MMP active site. The C-terminal domain of TIMP‑1 interacts with the C-terminal domain of pro‑MMP‑9, which may play a role in regulating pro‑MMP‑9 activation.

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Citations

1. Deletion of ADAM-9 in HGF/CDK4 mice impairs melanoma development and metastasis
   Authors: N Giebeler, A Schönefu beta, J Landsberg, T Tüting, C Mauch, P Zigrino
   Oncogene, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice
   Authors: MH Fan, Q Zhu, HH Li, HJ Ra, S Majumdar, DL Gulick, JA Jerome, DH Madsen, M Christofid, DW Speicher, WW Bachovchin, C Feghali-Bo, E Puré
   J. Biol. Chem., 2016;291(15):8070-89.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


3. TAp73 is essential for germ cell adhesion and maturation in testis.
   Authors: Holembowski L, Kramer D, Riedel D, Sordella R, Nemajerova A, Dobbelstein M, Moll U
   J Cell Biol, 2014;204(7):1173-90.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. The MMP-9/TIMP-1 axis controls the status of differentiation and function of myelin-forming Schwann cells in nerve regeneration.
   Authors: Kim Y, Remacle AG, Chernov AV
   PLoS ONE, 2012;7(3):e33664.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


5. Differential vesicular distribution and trafficking of MMP-2, MMP-9, and their inhibitors in astrocytes.
   Authors: Sbai O, Ould-Yahoui A, Ferhat L, Gueye Y, Bernard A, Charrat E, Mehanna A, Risso JJ, Chauvin JP, Fenouillet E, Rivera S, Khrestchatisky M
   Glia, 2010;58(3):344-66.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


6. Amelioration of coxsackievirus B3-mediated myocarditis by inhibition of tissue inhibitors of matrix metalloproteinase-1.
   Authors: Crocker SJ, Frausto RF, Whitmire JK, Benning N, Milner R, Whitton JL
   Am. J. Pathol., 2007;171(6):1762-73.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


7. Upregulation of MMP-9/TIMP-1 enzymatic system in eosinophilic meningitis caused by Angiostrongylus cantonensis.
   Authors: Chen KM, Lee HH, Chou HL, Liu JY, Tsai BC, Lai SC
   Int J Exp Pathol, 2005;86(2):81-9.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


8. Tissue inhibitor of metalloproteinase-1 deficiency amplifies acute lung injury in bleomycin-exposed mice.
   Authors: Kim KH, Burkhart K, Chen P, Frevert CW, Randolph-Habecker J, Hackman RC, Soloway PD, Madtes DK
   Am. J. Respir. Cell Mol. Biol., 2005;33(3):271-9.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


9. Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities.
   Authors: Thevenard J, Verzeaux L, Devy J, Etique N, Jeanne A, Schneider C, Hachet C, Ferracci G, David M, Martiny L, Charpentier E, Khrestchatisky M, Rivera S, Dedieu S, Emonard H
   PLoS ONE, 0;9(7):e103839.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB

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