抗TIMP-2抗体(Anti-TIMP-2, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	Chinese hamster ovary cell line CHO-derived recombinant human TIMP‑2Cys27-Pro220Accession # P16035
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human and mouse TIMP‑2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human TIMP-4 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize Recombinant Human TIMP-2 (0.143 µg/mL, Catalog # 971-TM) inhibition of Recombinant Human MMP‑2 (0.2 µg/mL, Catalog # 902-MPN or 902-MP) cleavage of the fluorogenic peptide substrate Mca‑PLGL‑Dpa‑AR‑NH2 (10 µM, Catalog # ES001). The Neutralization Dose (ND50) is typically 2.6 µg/mL.

Western Blot
	Detection of Human TIMP‑2 by Western Blot. Western blot shows Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse TIMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP‑2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	TIMP‑2 in Human Ovarian Cancer Tissue. TIMP‑2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human/Mouse TIMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization
	Neutralization of TIMP‑2 Activity by Human TIMP‑2 Antibody. Recombinant Human MMP‑2 (0.2 µg/mL, Catalog #902‑MPN or 902‑MP) activity is measured in the presence of Recombinant Human/Mouse TIMP-2 (0.143 µg/mL, Catalog # 971‑TM) that has been preincubated with increasing concentrations of Goat Anti-Human TIMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971). The ND50 is typically 2.6 µg/mL.

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Related Product & Information

Long Name	Tissue Inhibitors of Metalloproteinases 2
Entrez Gene IDs	7077 (Human); 21858 (Mouse); 29543 (Rat)
Background	TIMP-2
background_content	Background: TIMP-2 Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.

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Citations

1. Human umbilical cord plasma proteins revitalize hippocampal function in aged mice
   Authors: JM Castellano, KI Mosher, RJ Abbey, AA McBride, ML James, D Berdnik, JC Shen, B Zou, XS Xie, M Tingle, IV Hinkson, MS Angst, T Wyss-Coray
   Nature, 2017;544(7651):488-492.
   Species: Human
   Sample Type: Plasma
   Application: IP


2. Inhibition of airway epithelial-to-mesenchymal transition and fibrosis by kaempferol in endotoxin-induced epithelial cells and ovalbumin-sensitized mice.
   Authors: Gong J, Cho I, Shin D, Han S, Park S, Kang Y
   Lab Invest, 2014;94(3):297-308.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Mitral tissue inhibitor of metalloproteinase 2 is associated with mitral valve surgery outcome.
   Authors: Lin T, Yang S, Chiu C, Su H, Voon W, Chai C, Lai W, Sheu S
   PLoS ONE, 2014;9(1):e86287.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


4. Fibroblast-conditioned media promote human sarcoma cell invasion.
   Authors: Bittner JG, Wilson M, Shah MB, Albo D, Feig BW, Wang TN
   Surgery, 2009;145(1):42-7.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
   Authors: Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE
   J. Cell Biol., 2006;175(1):179-91.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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