抗Cathepsin B抗体(Anti-Cathepsin B, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin BHis18-Phe339Accession # P10605
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse Cathepsin B in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Cathepsin B is observed and less than 5% cross-reactivity with recombinant mouse Cathepsin H is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse Cathepsin B (Catalog # 965-CY)
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize Recombinant Mouse Cathepsin B (0.1 µg/mL, Catalog # 965-CY) cleavage of the fluorogenic peptide substrate Z-LR-AMC (10 µM, Catalog # ES008). The Neutralization Dose (ND50) is typically 0.33 µg/mL.

Immunohistochemistry

Cathepsin B in Mouse Thymus. Cathepsin B was detected in perfusion fixed frozen sections of mouse thymus using 5 µg/mL Goat Anti-Mouse Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF965) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

Cathepsin B

background_content

Background: Cathepsin B Cathepsin B is the first described member of the family of lysosomal cysteine proteases (1). Cathepsin B possesses both endopeptidase and exopeptidase activities, in the latter case acting as a peptidyl-dipeptidase. It is known to process a number of proteins, including pro and active caspases, prorenin and secretory leucoprotease inhibitor (SLPI) (2‑4). Therefore, Cathepsin B may play a role in activation and inactivation of caspases, activation of renin and inactivation of SLPI, the key steps in apoptosis, angiotensin production, and progression of emphysema, respectively. Because of its increased levels and redistribution in human and animal tumors, Cathepsin B may also have a role in invasion and metastasis (5). In addition to the lysosome, Cathepsin B can be secreted or associated with plasma membrane, cytoplasm, and nucleus. It is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme (5).

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Citations

1. Disruption of Small GTPase Rab7 Exacerbates the Severity of Acute Pancreatitis in Experimental Mouse Models
   Authors: K Takahashi, H Mashima, K Miura, D Maeda, A Goto, T Goto, GH Sun-Wada, Y Wada, H Ohnishi
   Sci Rep, 2017;7(1):2817.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


2. Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells.
   Authors: Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T
   Mol Cancer, 2015;14(0):39.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


3. Deficiency for the cysteine protease cathepsin L impairs Myc-induced tumorigenesis in a mouse model of pancreatic neuroendocrine cancer.
   Authors: Brindle N, Joyce J, Rostker F, Lawlor E, Swigart-Brown L, Evan G, Hanahan D, Shchors K
   PLoS ONE, 2015;10(4):e0120348.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-OCT embedded


4. Eucommia ulmoides Oliver extract, aucubin, and geniposide enhance lysosomal activity to regulate ER stress and hepatic lipid accumulation.
   Authors: Lee, Hwa-Youn, Lee, Geum-Hwa, Lee, Mi-Rin, Kim, Hye-Kyun, Kim, Nan-youn, Kim, Seung-Hy, Lee, Yong-Chu, Kim, Hyung-Ry, Chae, Han-Jung
   PLoS ONE, 2013;8(12):e81349.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC


5. Inflammasome activation by altered proteostasis.
   Authors: Shin, Jin Na, Fattah, Elmoataz, Bhattacharya, Abhisek, Ko, Soyoung, Eissa, N Tony
   J Biol Chem, 2013;288(50):35886-95.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


6. Macrophages and cathepsin proteases blunt chemotherapeutic response in breast cancer.
   Authors: Shree T, Olson OC, Elie BT
   Genes Dev., 2011;25(23):2465-79.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


7. Endocytosis provides a major alternative pathway for lysosomal biogenesis in kidney proximal tubular cells.
   Authors: Nielsen R, Courtoy PJ, Jacobsen C, Dom G, Lima WR, Jadot M, Willnow TE, Devuyst O, Christensen EI
   Proc. Natl. Acad. Sci. U.S.A., 2007;104(13):5407-12.
   Species: Mouse
   Sample Type: Serum
   Application: WB

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