抗GDF-15抗体(Anti-GDF-15, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Chinese hamster ovary cell line CHO-derived recombinant human GDF-15Ala197-Ile308Accession # Q99988
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human GDF-15 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 65% cross-reactivity with recombinant mouse (rm) GDF-15 is observed, and less than 1% cross-reactivity with rmGDF‑1, rmGDF-3, and rmGDF-11 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5 µg/mL	See below

Western Blot
	Detection of Human GDF‑15 by Western Blot. Western blot shows lysate of HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human GDF‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF957) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GDF‑15 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western
	Detection of Human GDF‑15 by Simple WesternTM. Simple Western lane view shows lysate of HT1080 human fibrosarcoma cell line, loaded at 0.2 mg/mL. A specific band was detected for GDF‑15 at approximately 47 kDa (as indicated) using 5 µg/mL of Goat Anti-Human GDF‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF957) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Background

GDF-15

background_content

Background: GDF-15 Growth Differentiation Factor 15 (GDF-15), also called Macrophage inhibitory cytokine-1 (MIC-1), placental transforming growth factor-beta, prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-beta ) superfamily. GDF-15 is highly expressed in placenta and is expressed at lower levels in kidney, pancreas, prostate and colon. It is also widely expressed in brain. Similar to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of GDF-15 contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Furthermore, the carboxy-terminal domain contains two additional cysteine residues that form a fourth intrachain disulfide bond. Biologically active GDF-15 is a disulfide-linked homodimer of the carboxy-terminal 112 amino acid residues. Mature human GDF-15 shares 66.1% and 68.7% amino acid sequence similarity with rat and mouse GDF-15, respectively, which are remarkably low homologies between species in TGF-beta superfamily. GDF-15 has been shown to have various functions, including inhibition of production of tumor necrosis factor alpha (TNF-alpha ) from lipopolysaccharide-stimulated macrophages, induction of cartilage formation, early-stage endochonadal bone formation, and promotion of neuronal survival.

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Citations

1. Panel of three novel serum markers predicts liver stiffness and fibrosis stages in patients with chronic liver disease
   Authors: M Krawczyk, S Zimmermann, G Hess, R Holz, M Dauer, J Raedle, F Lammert, F Grünhage
   PLoS ONE, 2017;12(3):e0173506.
   Species: Human
   Sample Type: Serum
   Application: ELISA Development


2. An autocrine/paracrine circuit of growth differentiation factor (GDF) 15 has a role for maintenance of breast cancer stem-like cells
   Authors: A Sasahara, K Tominaga, T Nishimura, M Yano, E Kiyokawa, M Noguchi, M Noguchi, H Kanauchi, T Ogawa, H Minato, K Tada, Y Seto, A Tojo, N Gotoh
   Oncotarget, 2017;8(15):24869-24881.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Growth Differentiation Factor-15-Induced Contractile Activity and Extracellular Matrix Production in Human Trabecular Meshwork Cells
   Invest. Ophthalmol. Vis. Sci, 2016;57(15):6482-6495.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. Identification of macrophage inhibitory cytokine-1 in adipose tissue and its secretion as an adipokine by human adipocytes.
   Authors: Ding Q, Mracek T, Gonzalez-Muniesa P, Kos K, Wilding J, Trayhurn P, Bing C
   Endocrinology, 2009;150(4):1688-96.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Prognostic value of growth-differentiation factor-15 in patients with non-ST-elevation acute coronary syndrome.
   Authors: Wollert KC, Kempf T, Peter T, Olofsson S, James S, Johnston N, Lindahl B, Horn-Wichmann R, Brabant G, Simoons ML, Armstrong PW, Califf RM, Drexler H, Wallentin L
   Circulation, 2007;115(8):962-71.
   Species: Human
   Sample Type: Serum
   Application: ELISA Development

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