抗Angiopoietin-1抗体(Anti-Angiopoietin-1, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-1Ser20-Phe498Accession # Q5HYA0
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human and mouse Angiopoietin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Angiopoietin-2 and rhAngiopoietin-4 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human Angiopoietin‑1 (Catalog # 923-AN)
Immunohistochemistry	5-15 µg/mL	See below

Immunohistochemistry
	Angiopoietin‑1 in Human Prostate Cancer Tissue. Angiopoietin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	Angiopoietin‑1 in Human Prostate. Angiopoietin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	Angiopoietin‑1 in Mouse Embryo. Angiopoietin‑1 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelium in the loop of the midgut. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

Angiopoietin-1

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Background: Angiopoietin-1 Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are two closely related secreted ligands which bind with similar affinity to Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature (1). Ang-1 cDNA encodes a 498 amino acid (aa) residue precursor protein that contains a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-1 shares approximately 97% and 60% amino acid sequence identity with mouse Ang-1 and human Ang-2, respectively (1, 2). Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization (1, 3, 4). Ang-2 has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context (5).

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Citations

1. The Fibrin Cleavage Product B?15-42 Channels Endothelial and Tubular Regeneration in the Post-acute Course During Murine Renal Ischemia Reperfusion Injury
   Authors: D Fischer, C Seifen, P Baer, M Jung, C Mertens, B Scheller, K Zacharowsk, R Hofmann, TJ Maier, A Urbschat
   Front Pharmacol, 2018;9(0):369.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


2. Ang-2 but not Ang-1 expression in perivascular soft tissue tumors
   Authors: Swati Shrestha
   J Orthop, 2017;14(1):147-153.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


3. Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers.
   Authors: Baruah P, Lee M, Wilson P, Odutoye T, Williamson P, Hyde N, Kaski J, Dumitriu I
   Br J Cancer, 2015;113(4):653-9.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


4. Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis.
   Authors: Brunckhorst MK, Wang H, Lu R, Yu Q
   Cancer Res., 2010;70(18):7283-93.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy.
   Authors: Dallabrida SM, Ismail NS, Pravda EA, Parodi EM, Dickie R, Durand EM, Lai J, Cassiola F, Rogers RA, Rupnick MA
   FASEB J., 2008;22(8):3010-23.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


6. Angiogenesis markers (VEGF, soluble receptor of VEGF and angiopoietin-1) in very early arthritis and their association with inflammation and joint destruction.
   Authors: Clavel G, Bessis N, Lemeiter D, Fardellone P, Mejjad O, Menard JF, Pouplin S, Boumier P, Vittecoq O, Le Loet X, Boissier MC
   Clin. Immunol., 2007;124(2):158-64.
   Species: Human
   Sample Type: Serum
   Application: ELISA Development


7. Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo.
   Authors: Asikainen TM, Waleh NS, Schneider BK, Clyman RI, White CW
   Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(4):L588-95.
   Species: Primate - Baboon
   Sample Type: Tissue Homogenates
   Application: WB


8. Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
   Authors: Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW
   Proc. Natl. Acad. Sci. U.S.A., 2005;102(29):10212-7.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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