抗Akt1/2/3抗体(Anti-Akt1/2/3, PDK2 Site, Phosphorylated, Human/Mouse/Rat, Rabbit-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	Phosphopeptide containing human, mouse, and rat Akt1 S473 site
Source	Polyclonal Rabbit IgG
Purification	Antigen and protein A Affinity-purified
Specificity	Detects human, mouse and rat Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	See below

Western Blot
	Detection of Mouse and Human Phospho-Akt (S473) Pan Specific by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	Akt in Human Breast Cancer Tissue. Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Intracellular Staining by Flow Cytometry
	Detection of Phospho-Akt (S473) in IGF-1-treated MCF‑7 cells by Flow Cytometry. Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF887) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Simple Western
	Detection of Human Phospho-Akt (S473) by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	v-Akt Murine Thymoma Viral Oncogene Homolog
Background	Akt
background_content	Background: Akt Akt1, Akt2, and Akt3 make up a family of serine/threonine kinases, often activated downstream of growth factor receptors and PI 3-Kinase. Each Akt family member contains an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain. They have putative roles in cell proliferation and survival, and Akt perturbations are thought to play an important role in tumorigenesis.

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Citations

1. Modeling Multiple Myeloma-Bone Marrow interactions and response to drugs in a 3D surrogate microenvironment
   Authors: D Belloni, S Heltai, M Ponzoni, A Villa, B Vergani, L Pecciarini, M Marcatti, S Girlanda, G Tonon, F Ciceri, F Caligaris-, M Ferrarini, E Ferrero
   Haematologica, 2018;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Subthalamic Nucleus Deep Brain Stimulation Employs trkB Signaling for Neuroprotection and Functional Restoration
   Authors: DL Fischer, CJ Kemp, A Cole-Strau, NK Polinski, KL Paumier, JW Lipton, K Steece-Col, TJ Collier, DJ Buhlinger, CE Sortwell
   J. Neurosci., 2017;37(28):6786-6796.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Frozen


3. Different dynamics of NLRP3 inflammasome-mediated IL?1? production in GM-CSF- and M-CSF-differentiated human macrophages
   Authors: MM Budai, J T?zsér, S Benk?
   J. Leukoc. Biol, 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Ocular Delivery of PACAP1-27 Protects the Retina From Ischemic Damage in Rodents
   Invest. Ophthalmol. Vis. Sci, 2016;57(15):6683-6691.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


5. Dual inhibition of CDK4/Rb and PI3K/AKT/mTOR pathways by ON123300 induces synthetic lethality in mantle cell lymphomas.
   Authors: Divakar S, Ramana Reddy M, Cosenza S, Baker S, Perumal D, Antonelli A, Brody J, Akula B, Parekh S, Premkumar Reddy E
   Leukemia, 2016;30(1):86-93.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Interferon-gamma suppresses intestinal epithelial aquaporin-1 expression via Janus kinase and STAT3 activation.
   Authors: Dicay M, Hirota C, Ronaghan N, Peplowski M, Zaheer R, Carati C, MacNaughton W
   PLoS ONE, 2015;10(3):e0118713.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


7. Water-filtered infrared a irradiation in combination with visible light inhibits acute chlamydial infection.
   Authors: Marti H, Koschwanez M, Pesch T, Blenn C, Borel N
   PLoS ONE, 2014;9(7):e102239.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Neuronal pentraxin 2 supports clear cell renal cell carcinoma by activating the AMPA-selective glutamate receptor-4.
   Authors: von Roemeling C, Radisky D, Marlow L, Cooper S, Grebe S, Anastasiadis P, Tun H, Copland J
   Cancer Res, 2014;74(17):4796-810.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. The role of nuclear beta-catenin accumulation in the Twist2-induced ovarian cancer EMT.
   Authors: Mao Y, Xu J, Li Z, Zhang N, Yin H, Liu Z
   PLoS ONE, 2013;8(11):e78200.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


10. An elaborate regulation of Mammalian target of rapamycin activity is required for somatic cell reprogramming induced by defined transcription factors.
    Authors: He, Jing, Kang, Lan, Wu, Tong, Zhang, Jiqin, Wang, Haitao, Gao, Haibo, Zhang, Yu, Huang, Bo, Liu, Wenqiang, Kou, Zhaohui, Zhang, Hongbing, Gao, Shaorong
    Stem Cells Dev, 2012;21(14):2630-41.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB

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