抗BMP Receptor 2抗体(Anti-BMP Receptor 2, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human BMPR-IIAla26-Ile151Accession # Q13873
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human BMPR-II in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) BMPR‑IA, recombinant mouse (rm) BMPR-IA, rhBMPR-IB, and rmBMPR-IB is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human BMPR-II Fc Chimera (Catalog # 811-BR)
Flow Cytometry	2.5 µg/106 cells	See below
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry
	BMPR-II in Human Prostate. BMPR-II was detected in formalin fixed paraffin-embedded sections of human prostate using Goat Anti-Human BMPR-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF811) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Flow Cytometry
	Detection of BMPR‑II in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Goat Anti-Human BMPR‑II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF811, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

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Related Product & Information

Background

BMPR-II

background_content

Background: BMPR-II Like other members of the TGF-beta superfamily, cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the binding to heteromeric complex of type I and type II serine-threonine kinase receptors. Both receptor types are required for the signal transduction. BMP receptor II (BMPR-II) is one of the five mammalian type II receptors (including TGF-beta R-II, ActR-II, ActR-IIB, BMPR-II, and MISR-II) for the TGF-beta superfamily ligands. The type II receptors for TGF-beta and activin bind ligands with high affinity by itself. In contrast, BMPR-II binds BMP-2, BMP-4, and BMP-7 weakly in the absence of type I receptor, and the binding can be facilitated by the presence of the type I receptor. BMPR-II mRNA is widely expressed in fetal and adult tissues. Human and mouse BMPR-II are highly conserved sharing 97% sequence identity (1-3).

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Citations

1. Endocytosis contributes to BMP2-induced Smad signalling and neuronal growth
   Authors: SV Hegarty, AM Sullivan, GW O'Keeffe
   Neurosci. Lett, 2017;643(0):32-37.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Bone morphogenetic protein-9 activates Smad and ERK pathways and supports human osteoclast function and survival in vitro.
   Authors: Fong D, Bisson M, Laberge G, McManus S, Grenier G, Faucheux N, Roux S
   Cell Signal, 2013;25(4):717-28.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Epigenetic repression of bone morphogenetic protein receptor II expression in scleroderma.
   Authors: Wang Y, Kahaleh B
   J Cell Mol Med, 2013;17(10):1291-9.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. BMP4 is a peripherally-derived factor for motor neurons and attenuates glutamate-induced excitotoxicity in vitro.
   Authors: Chou H, Lai D, Huang C, McLennan I, Wang H, Wang P
   PLoS ONE, 2013;8(3):e58441.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Bone morphogenetic protein receptor IB as a marker for enrichment of osteogenic precursor-like cells in human dermis.
   Authors: He J, Dong J, Wang T
   Arch. Dermatol. Res., 2011;303(8):581-90.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. High-density lipoproteins affect endothelial BMP-signaling by modulating expression of the activin-like kinase receptor 1 and 2.
   Authors: Yao Y, Shao ES, Jumabay M, Shahbazian A, Ji S, Bostrom KI
   Arterioscler. Thromb. Vasc. Biol., 2008;28(12):2266-74.
   Species: Human
   Sample Type: Whole Cells
   Application: WB


7. Cell origin in experimental repair of cricoid cartilage defects treated with recombinant human bone morphogenetic protein-2.
   Authors: Tcacencu I, Carlsoo B, Stierna P
   Wound Repair Regen, 2005;13(3):341-9.
   Species: Rabbit
   Sample Type: Whole Tissue
   Application: IHC

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